Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,092,330 G→A 6.2% A412T (GCC→ACC)  hisD → bifunctional histidinal dehydrogenase/ histidinol dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,092,3300GA 6.2% 201.5 / 2.6 64A412T (GCC→ACC) hisDbifunctional histidinal dehydrogenase/ histidinol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (29/31);  new base A (2/2);  total (31/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.71e-01

CCGGCACCAACCACGTTCTACCGACTTACGGTTACACCGCCACCTGTTCCAGCCTCGGGCTGGCAGATTTCCAGAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGG  >  NC_000913/2092184‑2092477
                                                                                                                                                  |                                                                                                                                                   
ccGGCACCAACCACGTTCTACCGACTTACGGTTACACCGCCACCTGTTCCAGCCTCGGGCTGGCAGATTTCCAGAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGgccgc                                                                                                                                                    >  7:163018/1‑148 (MQ=255)
   gCACCAACCACGTTCTACCGACTTACGGTTACACCGCCACCTGTTCCAGCCTCGGGCTGGCAGATTTCCAGAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGgccgccgc                                                                                                                                                 <  5:162975/148‑1 (MQ=255)
      ccaaccaCGTTCTACCGACTTACGGTTACACCGCCACCTGTTCCAGCCTCGGGCTGGCAGATTTCCAGAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGa                                                                                                                                              <  8:141886/148‑1 (MQ=255)
       caaccaCGTTCTACCGACTTACGGTTACACCGCCACCTGTTCCAGCCTCGGGCTGGCAGATTTCCAGAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAg                                                                                                                                             >  3:119983/1‑148 (MQ=255)
          ccaCGTTCTACCGACTTACGGTTACACCGCCACCTGTTCCAGCCTCGGGCTGGCAGATTTCCAGAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAgcgc                                                                                                                                          <  5:294586/148‑1 (MQ=255)
           caCGTTCTACCGACTTACGGTTACCCCGCCACCTGTTCCAGCCTCGGGCTGGCAGATTTCCAGAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGcc                                                                                                                                         >  5:90157/1‑148 (MQ=255)
                    ccGACTTACGGTTACACCGCCACCTGTTCCAGCCTCGGGCTGGCAGATTTCCAGAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCg                                                                                                                                   <  5:149967/145‑1 (MQ=255)
                      gACTTACGGTTACACCGCCACCTGTTCCAGCCTCGGGCTGGCAGATTTCCAGAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGccgccgccg                                                                                                                                               <  3:224480/131‑1 (MQ=255)
                      gACTTACGGTTACACCGCCACCTGTTCCAGCCTCGGGCTGGCAGATTTCCAGAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGccgccgccg                                                                                                                                               >  4:224480/1‑131 (MQ=255)
                       aCTTACGGTTACACCGCCACCTGTTCCAGCCTCGGGCTGGCAGATTTCCAGAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCcaca                                                                                                                             <  4:188856/148‑1 (MQ=255)
                            cGGTTACACCGCCACCTGTTCCAGCCTCGGGCTGGCAGATTTCCAGAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACaaaaa                                                                                                                         <  1:239920/147‑1 (MQ=255)
                                tACACCGCCACCTGTTCCAGCCTCGGGCTGGCAGATTTCCAGAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATg                                                                                                                       >  6:28776/1‑145 (MQ=255)
                                tACACCGCCACCTGTTCCAGCCTCGGGCTGGCAGATTTCCAGAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATg                                                                                                                       <  5:28776/145‑1 (MQ=255)
                                   acCGCCACCTGTTCCAGCCTCGGGCTGGCAGATTTCCAGAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGtt                                                                                                                  >  7:248103/1‑147 (MQ=255)
                                         aCCTGTTCCAGCCTCGGGCTGGCAGATTTCCAGAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGc                                                                                                           >  2:354731/1‑148 (MQ=255)
                                          ccTGTTCCAGCCTCGGGCTGGCAGATTTCCAGAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCg                                                                                                          <  1:354731/148‑1 (MQ=255)
                                           cTGTTCCAGCCTCGGGCTGGCAGATTTCCAGAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGTAACACTGGCCGCCGCCGAGCGCCTGACAGCCCACAAAAATGCCGTTACTTTGCgt                                                                                                         >  8:315969/1‑148 (MQ=255)
                                                   gCCTCGGGCTGGCAGATTTCCAGAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGc                                                                                                                      <  3:10722/127‑1 (MQ=255)
                                                   gCCTCGGGCTGGCAGATTTCCAGAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGc                                                                                                                      >  4:10722/1‑127 (MQ=255)
                                                   gCCTCGGGCTGGCAGATTTCCAGAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGc                                                                                                 <  7:188914/148‑1 (MQ=255)
                                                      tCGGGCTGGCAGATTTCCAGAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTa                                                                                                     <  7:327236/141‑1 (MQ=255)
                                                       cGGGCTGGCGGAGTTCCAGAAGCGCATGACCGGACGGGATCTGTCGAAAGAGGGGTTGTCCGCGCTGGCTTCCACCTTAGACCCACTGGCCGCCGCCGAGCGCCTGCCCGCCGACAAAAATGCCGGTACGTTGCGTGGTac                                                                                                    >  8:327236/1‑140 (MQ=255)
                                                       cGGGCTGGCAGATTTCCAGAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTa                                                                                                     <  5:200941/140‑1 (MQ=255)
                                                       cGGGCTGGCAGATTTCCAGAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTa                                                                                                     >  6:200941/1‑140 (MQ=255)
                                                        gggCTGGCAGATTTCCAGTAGCGAATGACCGTACAGGAACTGTCGAAAGATGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCtt                                                                                             <  6:186104/147‑1 (MQ=255)
                                                         ggCTGGCAGATTTCCAGAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTaa                                                                                           <  7:72986/148‑1 (MQ=255)
                                                              gCAGATTTCCAGAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGagc                                                                                      <  8:248103/148‑1 (MQ=255)
                                                                aGATTTCCAGAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGagcaa                                                                                    <  8:434869/148‑1 (MQ=255)
                                                                   tttCCAGAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGagcaagca                                                                                 <  3:224464/148‑1 (MQ=255)
                                                                   tttCCAGAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGagcaagca                                                                                 <  6:90157/148‑1 (MQ=255)
                                                                   tttCCAGAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGagcaagc                                                                                  <  8:163018/147‑1 (MQ=255)
                                                                         gAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCgtg                                                                                                        <  7:375469/119‑1 (MQ=255)
                                                                         gAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCgtg                                                                                                        >  8:375469/1‑119 (MQ=255)
                                                                              gcATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTg                                                                      <  8:190263/148‑1 (MQ=255)
                                                                               cATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCACCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAAc                                                                                                   >  3:255512/1‑118 (MQ=255)
                                                                               cATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCACCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAAc                                                                                                   >  3:249850/1‑118 (MQ=255)
                                                                               cATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCACCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAAc                                                                                                   <  4:255512/118‑1 (MQ=255)
                                                                               cATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCACCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAAc                                                                                                   <  4:249850/118‑1 (MQ=255)
                                                                                 tGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACt                                                                   <  4:83978/148‑1 (MQ=255)
                                                                                  gACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTa                                                                                            >  5:238416/1‑122 (MQ=255)
                                                                                  gACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTa                                                                                            <  6:238416/122‑1 (MQ=255)
                                                                                       tACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTAcc                                                             >  2:99941/1‑148 (MQ=255)
                                                                                        aCAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTAcc                                                             >  1:434498/1‑147 (MQ=255)
                                                                                        aCAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGGGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCg                                                            >  6:281104/1‑148 (MQ=255)
                                                                                           ggAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGATACTTTGCGTGGTAACGCCCGTAAGGAGCAAGCATGAGCACAGTGACTa                                                                  >  5:212813/1‑139 (MQ=255)
                                                                                              aCTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTg                                                                      >  6:219799/1‑132 (MQ=255)
                                                                                              aCTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTg                                                                      <  5:219799/132‑1 (MQ=255)
                                                                                              aCTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAg                                                      <  3:13297/148‑1 (MQ=255)
                                                                                                 gTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAgcgc                                                   >  1:315210/1‑148 (MQ=255)
                                                                                                      aaGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCg                                                                        <  2:50711/122‑1 (MQ=255)
                                                                                                      aaGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCg                                                                        >  1:50711/1‑122 (MQ=255)
                                                                                                      aaGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGa                                               >  1:338569/1‑147 (MQ=255)
                                                                                                            ggTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTc                                        <  2:434498/148‑1 (MQ=255)
                                                                                                              ttCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCg                                      >  1:40906/1‑148 (MQ=255)
                                                                                                               tctcCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCg                                      >  8:110542/1‑147 (MQ=255)
                                                                                                               tctcCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCg                                      >  1:197306/1‑147 (MQ=255)
                                                                                                                   cgcgCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCaa                                   <  7:315969/146‑1 (MQ=255)
                                                                                                                     cgcTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTg                               <  4:119983/148‑1 (MQ=255)
                                                                                                                         ggCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGc                           >  1:15689/1‑148 (MQ=255)
                                                                                                                             tCAACCATAGAAACCCTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTa                       >  2:330218/1‑148 (MQ=255)
                                                                                                                             tCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAAc                                  >  3:107161/1‑137 (MQ=255)
                                                                                                                             tCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAAc                                  <  4:107161/137‑1 (MQ=255)
                                                                                                                                   aTAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGCCTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTc                 >  5:35791/1‑148 (MQ=255)
                                                                                                                                     aGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCgg               >  4:41076/1‑148 (MQ=255)
                                                                                                                                      gAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCgg               <  3:298305/147‑1 (MQ=255)
                                                                                                                                      gAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGgc              >  6:309521/1‑148 (MQ=255)
                                                                                                                                          cacTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGcgt          <  2:338569/148‑1 (MQ=255)
                                                                                                                                              ggCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCgtcgtc      >  8:417269/1‑148 (MQ=255)
                                                                                                                                               gccgccgccGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCgtcgtc      <  7:110542/147‑1 (MQ=255)
                                                                                                                                                 cgccgccgAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGaa                                              >  6:223515/1‑105 (MQ=255)
                                                                                                                                                 cgccgccgAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGaa                                              <  5:223515/105‑1 (MQ=255)
                                                                                                                                                  gccgccgAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCCAGCATGAGCCCCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTggg  >  3:12135/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CCGGCACCAACCACGTTCTACCGACTTACGGTTACACCGCCACCTGTTCCAGCCTCGGGCTGGCAGATTTCCAGAAGCGCATGACCGTACAGGAACTGTCGAAAGAGGGGTTCTCCGCGCTGGCTTCAACCATAGAAACACTGGCCGCCGCCGAGCGCCTGACCGCCCACAAAAATGCCGTTACTTTGCGTGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGG  >  NC_000913/2092184‑2092477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑