Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,762,221 G→T 5.1% I440I (ATC→ATA yfjK ← radiation resistance protein; DEAD/H helicase‑like protein; CP4‑57 putative defective prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,762,2210GT 5.1% 459.6 / 8.5 131I440I (ATC→ATAyfjKradiation resistance protein; DEAD/H helicase‑like protein; CP4‑57 putative defective prophage
Reads supporting (aligned to +/- strand):  ref base G (59/65);  new base T (5/2);  total (64/67)
Fisher's exact test for biased strand distribution p-value = 2.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-01

Only 100 of 131 total aligned reads displayed.

CATCAAAGCGATCCTGTGAAAACTCGGACAAGTGGTCTGGTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTGACTCCTTCAATGATCGTGG  >  NC_000913/2762076‑2762366
                                                                                                                                                 |                                                                                                                                                 
 atcaAAGCGATCCTGTGAAAACTCGGACAAGTGGTCTGGTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGAtt                                                                                                                                                <  3:270766/148‑1 (MQ=255)
 atcaAAGCGATCCTGTGAAAACTCGGACAAGTGGTCTGGTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGAt                                                                                                                                                 <  8:245348/147‑1 (MQ=255)
   caAAGCGATCCTGTGAAAACTCGGACAAGTGGTCTGGTTGAACGCTGGCTAACAGTTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGAttt                                                                                                                                               >  2:77498/1‑147 (MQ=255)
   caAAGCGATCCTGTGAAAACTCGGACAAGTGGTCTGGTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGAttt                                                                                                                                               <  1:77498/147‑1 (MQ=255)
           tCCTGTGAAAACTCGGACAAGTGGTCTGGTTGAACTCTGGCTAACAGGTTGATTGTGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCcgac                                                                                                                                        <  1:16675/146‑1 (MQ=255)
             cTGTGAAAACTCGGACAAGTGGTCTGGTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGaa                                                                                                                                     <  7:273322/147‑1 (MQ=255)
                tgAAAACTCGGACAAGTGGTCTGGTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGa                                                                                                                                 <  4:379319/148‑1 (MQ=255)
                  aaaaCTCGGACAAGTGGTCTGGTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAAc                                                                                                                               >  4:297471/1‑148 (MQ=255)
                   aaaCTCGGACAAGTGGTCTGGTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACg                                                                                                                              >  1:50316/1‑148 (MQ=255)
                      cTCGGACAAGTGGTCTGGTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGccc                                                                                                                           <  2:25303/148‑1 (MQ=255)
                      cTCGGACAAGTGGTCTGGTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGcc                                                                                                                            >  5:178443/1‑147 (MQ=255)
                         ggACAAGTGGTCTGGTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCa                                                                                                                          <  4:208065/146‑1 (MQ=255)
                          gACAAGTGGTCTGGTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGAtat                                                                                                                                               >  4:262884/1‑122 (MQ=255)
                            cAAGTGGTCTGGTTTAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATgcgc                                                                                                                     <  6:123153/148‑1 (MQ=255)
                            cAAGTGGTCTGGTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATgcgc                                                                                                                     <  3:297471/148‑1 (MQ=255)
                                    cTGGTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGcgcg                                                                                                             <  7:275148/148‑1 (MQ=255)
                                      ggTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTg                                                                                                                                  <  7:434065/125‑1 (MQ=255)
                                      ggTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTg                                                                                                                                  >  8:434065/1‑125 (MQ=255)
                                      ggTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGa                                                                                                                                 >  8:209886/1‑126 (MQ=255)
                                      ggTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGa                                                                                                                                 <  7:209886/126‑1 (MQ=255)
                                        ttGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCt                                                                                                         >  8:197727/1‑148 (MQ=255)
                                         tGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCcg                                                                                                                                          >  8:445569/1‑114 (MQ=255)
                                         tGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCcg                                                                                                                                          <  7:445569/114‑1 (MQ=255)
                                          gAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCttt                                                                                                       >  6:409155/1‑148 (MQ=255)
                                           aaCGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCttt                                                                                                       >  5:101312/1‑147 (MQ=255)
                                                   cTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTccc                                                                                              <  3:288276/148‑1 (MQ=255)
                                                   cTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTcc                                                                                               >  8:140046/1‑147 (MQ=255)
                                                       cAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATgg                                                                                          <  5:409155/148‑1 (MQ=255)
                                                         ggTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGt                                                                                        <  8:32774/148‑1 (MQ=255)
                                                           ttGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGtt                                                                                                 <  5:197610/137‑1 (MQ=255)
                                                           ttGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGtt                                                                                                 >  6:197610/1‑137 (MQ=255)
                                                                tGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTg                                                                                 >  7:230904/1‑148 (MQ=255)
                                                                 ggggTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTg                                                                                 <  6:101312/147‑1 (MQ=255)
                                                                   ggTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCa                                                                              <  6:178443/148‑1 (MQ=255)
                                                                     tATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAAt                                                                            >  1:176326/1‑148 (MQ=255)
                                                                      aTCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGa                                                                                                                                 <  1:118598/94‑1 (MQ=255)
                                                                      aTCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTccc                                                                                              <  8:132273/129‑1 (MQ=255)
                                                                      aTCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTccc                                                                                              >  7:132273/1‑129 (MQ=255)
                                                                      aTCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTc                                                                               >  6:320457/1‑144 (MQ=255)
                                                                      aTCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTc                                                                               <  5:320457/144‑1 (MQ=255)
                                                                        cAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATgcgc                                                                                                                     >  7:331207/1‑104 (MQ=255)
                                                                        cAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATgcgc                                                                                                                     <  8:331207/104‑1 (MQ=255)
                                                                        cAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGCCCTTTGATGTTCCCATGGGTGAATTTGTCAAtgct                                                                         >  6:11247/1‑148 (MQ=255)
                                                                          aTGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAAtgctgc                                                                       >  2:36656/1‑148 (MQ=255)
                                                                           tGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGGCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCg                                                                      <  7:140046/148‑1 (MQ=255)
                                                                                tGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTg                                                                                                      >  2:24979/1‑111 (MQ=255)
                                                                                 gAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTc                                                                 >  2:443682/1‑147 (MQ=255)
                                                                                 gAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTc                                                                 >  8:101890/1‑147 (MQ=255)
                                                                                 gAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTc                                                                 >  8:202253/1‑147 (MQ=255)
                                                                                       cGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATcgcg                                                          >  1:327915/1‑148 (MQ=255)
                                                                                            ggAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGt                                                     <  2:50316/148‑1 (MQ=255)
                                                                                                 atCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTccc                                                                                              <  6:132026/102‑1 (MQ=255)
                                                                                                 atCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCg                                                                      >  7:281621/1‑126 (MQ=255)
                                                                                                 atCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCg                                                   >  8:346859/1‑145 (MQ=255)
                                                                                                 atCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCg                                                   >  5:165565/1‑145 (MQ=255)
                                                                                                 atCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCg                                                   >  1:277684/1‑145 (MQ=255)
                                                                                                  tCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAg                                                <  3:370052/147‑1 (MQ=255)
                                                                                                   ccACATCTTGATTGAGCTTGTCGTCCGGGATTTCCTCGAGGCAAACGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAAt                                                                            <  3:34353/118‑1 (MQ=255)
                                                                                                    cACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGt                                                  >  4:218933/1‑143 (MQ=255)
                                                                                                    cACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGt                                                  <  3:218933/143‑1 (MQ=255)
                                                                                                    cACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGAt                                              >  7:250811/1‑147 (MQ=255)
                                                                                                    aaCCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAATATTTTGCCGACGAAGTGAACCCCCATTCTCCCGGAGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAAtg                                                                           >  4:34353/2‑118 (MQ=255)
                                                                                                     aCCTCTTGATTGAGATTGTCATCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATca                                            <  5:240283/148‑1 (MQ=255)
                                                                                                          ttgattgaGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACaa                                         <  8:250811/146‑1 (MQ=255)
                                                                                                               tgaGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAAtgctg                                                                        >  3:127464/1‑110 (MQ=255)
                                                                                                               tgaGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAAtgctg                                                                        <  4:127464/110‑1 (MQ=255)
                                                                                                               tgaGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCt                                   >  6:206607/1‑147 (MQ=255)
                                                                                                                gagaTTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCt                                   >  5:16126/1‑146 (MQ=255)
                                                                                                                gagaTTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCt                                   >  7:353940/1‑146 (MQ=255)
                                                                                                                 agaTTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTa                                 <  2:176326/147‑1 (MQ=255)
                                                                                                                  gaTTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGa                                                                                                                                 >  5:382260/1‑50 (MQ=255)
                                                                                                                  gaTTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGa                                                                                                                                 <  6:382260/50‑1 (MQ=255)
                                                                                                                      gTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGAt                            <  7:346859/147‑1 (MQ=255)
                                                                                                                       tCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAg                          <  1:36656/148‑1 (MQ=255)
                                                                                                                        cTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAgt                         >  1:231869/1‑148 (MQ=255)
                                                                                                                          tCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTcc                                                                <  8:116196/107‑1 (MQ=255)
                                                                                                                          tCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTcc                                                                >  7:116196/1‑107 (MQ=255)
                                                                                                                          tCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTc                                    <  8:434407/135‑1 (MQ=255)
                                                                                                                          tCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTc                                    >  7:434407/1‑135 (MQ=255)
                                                                                                                          tCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAgtgt                       >  1:71014/1‑148 (MQ=255)
                                                                                                                           ccGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAgtg                        <  7:202253/146‑1 (MQ=255)
                                                                                                                           ccGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAgtg                        <  3:283984/146‑1 (MQ=255)
                                                                                                                           ccGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGtt                      >  1:428159/1‑148 (MQ=255)
                                                                                                                            cGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTg                     <  5:206607/148‑1 (MQ=255)
                                                                                                                            cGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTg                     <  8:230904/148‑1 (MQ=255)
                                                                                                                            cGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTg                     <  5:11247/148‑1 (MQ=255)
                                                                                                                                  ttCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTGACTCCt               >  3:36357/1‑148 (MQ=255)
                                                                                                                                    cctccAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTGACTCCtt              <  2:71014/147‑1 (MQ=255)
                                                                                                                                     ctccAGGCAAAAGATTTTTCCTACGAAGTTAACGCCCATGCTCCCTGCGCGACCTTTGATGTTCCCATGTGTGAATTTGTCAATGCTTCGATTTCCATCGCGGTTTTCGTAGATCACAACGTTCTTAGCGATAGTGTTGACTCCTTCa            <  3:96289/148‑1 (MQ=255)
                                                                                                                                     ctccAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTGACTCCTTCa            <  4:36357/148‑1 (MQ=255)
                                                                                                                                      tccAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTc                                                    >  1:352088/1‑107 (MQ=255)
                                                                                                                                         cggCAAAATCTTTTGCCGACGAAGTGACCGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGGAGAGCACAACGTTCTTAGCGATAGTGTGGACTCCTTCAATGa        >  1:97590/2‑148 (MQ=255)
                                                                                                                                         cggCAAAATCTTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTGACTCCTTCAATGa        >  6:107359/2‑148 (MQ=255)
                                                                                                                                         cggCAAAATCTTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTGACTCCTTCAATGa        >  6:101287/2‑148 (MQ=255)
                                                                                                                                            cAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTGACTCCTTCAATGATCg     <  2:327915/148‑1 (MQ=255)
                                                                                                                                             aaaaGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTGACTCCTTCAATGATCGt    >  3:2580/1‑148 (MQ=255)
                                                                                                                                               aaGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTGGTAGATGACAACGTTCTTAGCGATAGTCTTGACTCCTTCAATGCTCGTg   >  3:228601/1‑147 (MQ=255)
                                                                                                                                               aaGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTg                     >  5:120546/1‑129 (MQ=255)
                                                                                                                                               aaGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTGACTCCTTCAATGATCGTgg  <  5:117500/148‑1 (MQ=255)
                                                                                                                                               aaGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGAACTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTg                     <  6:120546/129‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CATCAAAGCGATCCTGTGAAAACTCGGACAAGTGGTCTGGTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTGACTCCTTCAATGATCGTGG  >  NC_000913/2762076‑2762366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: