Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,269,970 A→G 8.6% intergenic (+368/+246) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,269,9700AG 8.6% 149.0 / 5.8 58intergenic (+368/+246)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base A (23/30);  new base G (2/3);  total (25/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.75e-01

AATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTG  >  NC_000913/3269823‑3270113
                                                                                                                                                    |                                                                                                                                               
aaTAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGaa                                                                                                                                                 <  7:194173/148‑1 (MQ=255)
 aTAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAg                                                                                                                                                >  7:215038/1‑148 (MQ=255)
      gcgcACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGt                                                                                                                                           <  4:136030/148‑1 (MQ=255)
            tCACCAATCTGAAACCCGCCGGAACGGTTTATTACGGACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGttt                                                                                                                                         <  5:3570/144‑1 (MQ=255)
             caacaaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGTCAGTCATTCATCTTTCTGCCCCGCCCAAAGTAAAAACCCGCCGAAGCGGGgttg                                                                                                                                        >  6:3570/1‑143 (MQ=255)
             caacaaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGAAAAAACCCGCCGAAGCGGGTTTTTaag                                                                                                                                    >  5:251539/1‑146 (MQ=255)
              aacaaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGTAAAAACCCGCTTCGGCGGGTTTTTAct                                                                                                                                    <  2:163753/147‑2 (MQ=17)
               acaaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAGACCTCTTTGGGCCCCTCCAAAAGTAAAAACCCGCCGAAGGGGGGTTTTAcct                                                                                                                                   >  7:9012/1‑145 (MQ=17)
                 aaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGGGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTaaa                                                                                                                                <  1:181489/148‑1 (MQ=255)
                       aaaCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAAc                                                                                                                              <  4:109440/144‑1 (MQ=255)
                       aaaCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACa                                                                                                                             <  5:232892/145‑1 (MQ=255)
                       aaaCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACa                                                                                                                             <  6:415670/145‑1 (MQ=255)
                       aaaCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAgg                                                                                                                           <  8:199276/147‑1 (MQ=255)
                        aaCCCGCCGGAACGGTTTTTTACGTAAATAAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGTTATAGTGTCGTGGACAGTCATTCATCTTTCTTCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTg                                                                                                                         <  6:251539/148‑1 (MQ=11)
                          cccGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGaa                                                                                                                       <  2:193411/148‑1 (MQ=255)
                          cccGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGaa                                                                                                                       <  8:215038/148‑1 (MQ=255)
                              ccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTTTTGTGTAGTGGACATGATATCTGCTTTCTGCCCGGCAAAAAGATTAAACCCTCAGAAGCTGGTTTTTACTTAATACGGGTGaaccgg                                                                                                                   >  3:108363/1‑144 (MQ=12)
                               cgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCCAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGa                                                                                                                  <  4:384490/148‑1 (MQ=255)
                                  aaCGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAgg                                                                                                                           >  2:325866/1‑136 (MQ=255)
                                  aaCGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAgg                                                                                                                           <  1:325866/136‑1 (MQ=255)
                                    cGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGAt                                                                                                             <  2:344125/148‑1 (MQ=255)
                                            taggTACATCAGGTAACAATTACCGATAAGCCGCATGCATTTTTGTCAAGTGTCGTCGACAGGCCTTCATATTTAGGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTACAACAGGTGAAACTGACCGATAAGCCGc                                                                                                      <  3:266759/144‑1 (MQ=14)
                                              cGTACATCATGTATTAATGTCCGATAAGTTGTTTTCTTTTTTGTATAGTTTCGTGGACAGTGTTTATTATTACTGCCCCTCCAAAATTAAAAACCCGCCGAACCGGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCGGctt                                                                                                    <  6:410618/147‑1 (MQ=2)
                                              CGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAc                                                                                                                 <  2:241898‑M1/134‑1 (MQ=255)
                                              CGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAc                                                                                                                 >  1:241898‑M1/1‑134 (MQ=255)
                                                 aCATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGaaa                                                                                                                      >  2:239667/1‑126 (MQ=255)
                                                 aCATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGaaa                                                                                                                      <  1:239667/126‑1 (MQ=255)
                                                  cATCAGCTAAAACTGACCGATTAGCCGCCTTCCTTTGGCTATTGTGTCGTGGACAGTCTTTTTTCTATCTGCCCCTCCAAAAGTACAAACCCGCCGCATCGGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctttcttt                                                                                               <  4:206241/148‑1 (MQ=14)
                                                             aCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGa                                                                                                              <  6:123602/122‑1 (MQ=35)
                                                             aCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGa                                                                                                              >  5:123602/1‑122 (MQ=35)
                                                                                    ttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTaaa                                                                                                                                <  6:175410/81‑1 (MQ=33)
                                                                                    ttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTaaa                                                                                                                                >  5:175410/1‑81 (MQ=33)
                                                                                        gTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctttcttt                                                                                               >  1:312323/1‑110 (MQ=35)
                                                                                         tataGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAc                                                                            <  1:237703/128‑1 (MQ=38)
                                                                                          ataGTGTCGTGGACAGTCATTCATCTATCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAAAGATAAGACGCTTTCTTTTGGGTATAGTGTCGTGGACa                                                                           >  2:237703/1‑128 (MQ=255)
                                                                                                             ttcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctttcttt                                                                                               >  5:193252/1‑89 (MQ=38)
                                                                                                             ttcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctttcttt                                                                                               <  6:193252/89‑1 (MQ=38)
                                                                                                             ttcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg                                                                                            >  2:200154/1‑92 (MQ=37)
                                                                                                             ttcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTc                                                                                  >  5:169278/1‑102 (MQ=17)
                                                                                                             ttcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTc                                                                                  <  6:169278/102‑1 (MQ=17)
                                                                                                               tcatCTTTCTGTCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGaa                                                                                                                       >  1:177179/1‑64 (MQ=255)
                                                                                                               tcatCTTTCTGTCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGaa                                                                                                                       <  2:177179/64‑1 (MQ=255)
                                                                                                               tcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtcatt                                                                     <  1:440494/114‑1 (MQ=39)
                                                                                                               tcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtcatt                                                                     >  2:440494/1‑114 (MQ=39)
                                                                                                               tcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgcc                                   >  8:81051/1‑148 (MQ=37)
                                                                                                                  tcagtttcacctgttttacgtaaAAACCCGCTTCGGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtcattca                                                                   <  4:216746‑M2/90‑1 (MQ=255)
                                                                                                                  tcagtttcacctgttttacgtaaAAACCCGCTTCGGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtcattca                                                                   >  3:216746‑M2/24‑113 (MQ=255)
                                                                                                                         cacctgttttacgtaaAAACCCGCTTCGGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtc                                                                        <  7:334297‑M2/85‑1 (MQ=255)
                                                                                                                         cacctgttttacgtaaAAACCCGCTTCGGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtc                                                                        >  8:334297‑M2/17‑101 (MQ=255)
                                                                                                                              tCCAAAAGAAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCCCTAACATTTGGGTATAGTGTCGTGGACAGTCAAACATCTTTCAGCCCCTCCAAAAGCaaaa                                          <  5:108811/126‑1 (MQ=11)
                                                                                                                               ccAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTc                                                             <  7:243667/106‑1 (MQ=40)
                                                                                                                               ccAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTc                                                             <  2:163647/106‑1 (MQ=40)
                                                                                                                               ccAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTc                                                             >  8:243667/1‑106 (MQ=40)
                                                                                                                               ccAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTc                                                             >  1:163647/1‑106 (MQ=40)
                                                                                                                                 aaaaGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctttc                                                                                                  >  4:264703/1‑67 (MQ=40)
                                                                                                                                         aaaCCCGCCGAAGCGGGTTTTTATGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCtt                                                               <  6:389760/94‑1 (MQ=37)
                                                                                                                                         aaaCCCGCCGAAGCGGGTTTTTATGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCtt                                                               >  5:389760/1‑94 (MQ=37)
                                                                                                                                         aaaCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGaa                                                                                                                       >  5:138618/1‑38 (MQ=40)
                                                                                                                                                ccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTg  <  7:81051/148‑1 (MQ=37)
                                                                                                                                                    |                                                                                                                                               
AATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTG  >  NC_000913/3269823‑3270113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: