Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,507,472 T→C 5.2% K77R (AAA→AGA)  yhfU ← DUF2620 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,507,4720TC 5.2% 316.6 / 2.5 101K77R (AAA→AGA) yhfUDUF2620 family protein
Reads supporting (aligned to +/- strand):  ref base T (47/47);  new base C (2/3);  total (49/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

ATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGTGAATGAAA  >  NC_000913/3507325‑3507618
                                                                                                                                                   |                                                                                                                                                  
attcAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATAATTTTGGAGATATGCTCCTCTTTCGCATTAATGCCTGGtt                                                                                                                                                    >  4:196689/4‑148 (MQ=255)
   cAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCGTGGTTTgg                                                                                                                                                 <  6:251207‑M2/148‑3 (MQ=255)
   cAGATACATAGTCGTGCGTTTTAATTCAGATGGTTAATCAGCATCGGAAACGCGTGTTCGACGTGCTCAACGCAAAGCCCAAACGCCACTTTTCCTTCAGAGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTATgg                                                                                                                                                 <  8:443655‑M2/148‑3 (MQ=255)
       tCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAAt                                                                                                                                             >  4:361930/1‑148 (MQ=255)
         cATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATg                                                                                                                                            <  1:123402/147‑1 (MQ=255)
                   ccTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACttt                                                                                                                                  <  6:288483/147‑1 (MQ=255)
                    cTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTg                                                                                                                                <  2:63922/148‑1 (MQ=255)
                    cTTTTATTTCAGATGGTTAATAAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTg                                                                                                                                >  4:138710/1‑148 (MQ=255)
                      tttATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATTATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGt                                                                                                                               >  5:170478/1‑147 (MQ=255)
                      tttATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCAAACCAggcattaaagcgaaagacgag                                                                                                                              <  1:447826‑M2/148‑22 (MQ=255)
                          tttCAGATTGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAg                                                                                                                          <  1:271266/148‑1 (MQ=255)
                            tCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGc                                                                                                                         <  2:247570/147‑1 (MQ=255)
                              aGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCt                                                                                                                       <  4:116417/147‑1 (MQ=255)
                              aGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTa                                                                                                                      >  7:2694/1‑148 (MQ=255)
                              aGATGCTTAATCAGCATCGGAATCGCGTGATCGACGTGCTCAACGGAAAGCCCAAACGCCACTTTTCCATCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAAAGCCTGGTATGGCAATGGTGCAACTTTTGTTATAGCCt                                                                                                                       <  3:196689/147‑1 (MQ=255)
                                aTGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATc                                                                                                                    <  5:251207/148‑1 (MQ=255)
                                   gTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCAcc                                                                                                                 >  7:411334/1‑148 (MQ=255)
                                      aaTCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACcgc                                                                                                               <  3:361930/147‑1 (MQ=255)
                                       aTCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACcgcg                                                                                                              <  3:368043/147‑1 (MQ=255)
                                        tCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCACTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGAt                                                                                                            <  7:69895/148‑1 (MQ=255)
                                          aGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATTATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATg                                                                                                           <  6:170478/147‑1 (MQ=255)
                                          aGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATg                                                                                                           <  8:2694/147‑1 (MQ=255)
                                             aTCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCaa                                                                                                       >  8:158208/1‑148 (MQ=255)
                                              tCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAAt                                                                                                      <  3:175697/148‑1 (MQ=255)
                                               cGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAAt                                                                                                      >  5:52093/1‑147 (MQ=255)
                                               cGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAAt                                                                                                      <  6:52093/147‑1 (MQ=255)
                                                  aaTCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACtt                                                                                                                                   >  7:174071/1‑115 (MQ=255)
                                                  aaTCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACtt                                                                                                                                   <  8:174071/115‑1 (MQ=255)
                                                     cgcgTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCAAACCAggcattaaagcgaaagacgagcatatcgccaaaatga                                                                                                              >  2:289734‑M2/1‑96 (MQ=255)
                                                     cgcgTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCAAACCAggcattaaagcgaaagacgagcatatcgccaaaatga                                                                                                              <  1:289734‑M2/133‑38 (MQ=255)
                                                      gcgTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAAt                                                                                               <  7:158208/147‑1 (MQ=255)
                                                      gcgTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCAAACCAggcattaaagagaaagacgagcatatcgccaaaatgatcgctgaaggaaaagt                                                                                              >  2:447826‑M2/1‑95 (MQ=255)
                                                                cGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGcc                                                                                                                        <  2:187676/112‑1 (MQ=255)
                                                                cGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGcc                                                                                                                        >  1:187676/1‑112 (MQ=255)
                                                                  tGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTg                                                                                                                                >  4:253783/1‑102 (MQ=255)
                                                                  tGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTg                                                                                                                                <  3:253783/102‑1 (MQ=255)
                                                                     tCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACcgcg                                                                                                              >  1:114738/1‑117 (MQ=255)
                                                                     tCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACcgcg                                                                                                              <  2:114738/117‑1 (MQ=255)
                                                                        aCGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTAt                                                                            >  1:103146/1‑148 (MQ=255)
                                                                        aCGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGAACCGGTAt                                                                            >  1:212681/1‑148 (MQ=255)
                                                                             aaGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCTTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTAAAGCCTATCAACGCGATGCCACTCGACAATGCCGAGCCCGCACCGGAATTacac                                                                       >  5:265488/1‑148 (MQ=255)
                                                                             aaGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACtttt                                                                                                                                 >  8:333343/1‑90 (MQ=255)
                                                                             aaGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTacac                                                                       >  5:242000/1‑148 (MQ=255)
                                                                                   aaaCGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGcc                                                                  >  2:86644/1‑147 (MQ=255)
                                                                                    aaCGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGa                                                                >  5:6516/1‑148 (MQ=255)
                                                                                    aaCGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGa                                                                >  3:92844/1‑148 (MQ=255)
                                                                                    aaCGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGa                                                                >  3:98507/1‑148 (MQ=255)
                                                                                    aaCGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGa                                                                >  7:233100/1‑148 (MQ=255)
                                                                                     aCGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGAt                                                               <  1:86644/148‑1 (MQ=255)
                                                                                      cGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACAGCGATGGCAATCGAAAATGCCGCGACCTCACCGGTATTACACGCGCCGATg                                                              >  1:342284/1‑148 (MQ=255)
                                                                                       gcaaCTTTTCCTTCAGAGATCATTTTGGCGATATGCTCGTCTGTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGt                                                             <  6:6516/145‑1 (MQ=255)
                                                                                        ccACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGta                                                            <  8:412452/148‑1 (MQ=255)
                                                                                        ccACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGta                                                            <  4:98507/148‑1 (MQ=255)
                                                                                        ccACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGt                                                             >  2:433447/1‑147 (MQ=255)
                                                                                          aCTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGtaa                                                           >  6:134878/1‑147 (MQ=255)
                                                                                             tttCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGc                                                                                             <  1:306417/110‑1 (MQ=255)
                                                                                             tttCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGc                                                                                             >  2:306417/1‑110 (MQ=255)
                                                                                             tttCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGta                                                            <  5:340321/143‑1 (MQ=255)
                                                                                             tttCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGta                                                            >  6:340321/1‑143 (MQ=255)
                                                                                             tttCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTaataat                                                       >  1:205237/1‑148 (MQ=255)
                                                                                              ttCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTAATAATc                                                      >  4:28025/1‑148 (MQ=255)
                                                                                                ccTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTAATAATcc                                                     <  8:411334/147‑1 (MQ=255)
                                                                                                 cTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGta                                                            <  1:321369/139‑1 (MQ=255)
                                                                                                 cTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGta                                                            >  2:321369/1‑139 (MQ=255)
                                                                                                  ttCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCg                                                                 <  8:256547/133‑1 (MQ=255)
                                                                                                  ttCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCg                                                                 >  7:256547/1‑133 (MQ=255)
                                                                                                     aGCGAGCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTCTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTAATAATCCAGTTGc                                               <  7:443655/148‑1 (MQ=255)
                                                                                                         aTCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGcc                                                                  >  6:25211/1‑125 (MQ=255)
                                                                                                         aTCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGcc                                                                  <  5:25211/125‑1 (MQ=255)
                                                                                                             ttttGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTAATAATCCAGTTGCCCGGAttt                                       <  2:103146/148‑1 (MQ=255)
                                                                                                              tttGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGAt                                                               >  8:163442/1‑123 (MQ=255)
                                                                                                              tttGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGAt                                                               <  7:163442/123‑1 (MQ=255)
                                                                                                              tttGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTAATAATCCAGTTGCCCGGAttt                                       >  7:61478/1‑147 (MQ=255)
                                                                                                                  gCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCt                                  >  4:114166/1‑148 (MQ=255)
                                                                                                                    gATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTc                                <  7:22002/148‑1 (MQ=255)
                                                                                                                    gATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTc                                <  8:233100/148‑1 (MQ=255)
                                                                                                                      tatGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCAt                              <  3:138710/148‑1 (MQ=255)
                                                                                                                           tCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTATTAATCCAGTTGCCCGGATTTCa                                     <  8:451029/136‑1 (MQ=255)
                                                                                                                           tCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTACTAATCCAGTTGCCCGGATTTCa                                     >  7:451029/1‑136 (MQ=255)
                                                                                                                           tCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGAACCGGTATTACAAGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCg                         >  3:337275/1‑148 (MQ=255)
                                                                                                                            cGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGc                        >  8:55075/1‑148 (MQ=255)
                                                                                                                             gTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTac                                                                         >  2:322987/1‑98 (MQ=255)
                                                                                                                             gTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTac                                                                         <  1:322987/98‑1 (MQ=255)
                                                                                                                                 ttCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCa                   >  3:59959/1‑148 (MQ=255)
                                                                                                                                  tCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTcc                    <  7:55075/146‑1 (MQ=255)
                                                                                                                                  tCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTcc                    <  2:205237/146‑1 (MQ=255)
                                                                                                                                    gCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTcc                    >  8:441936/1‑144 (MQ=255)
                                                                                                                                    gCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTcc                    <  7:441936/144‑1 (MQ=255)
                                                                                                                                    gCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGt                <  1:433447/148‑1 (MQ=255)
                                                                                                                                       ttAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATGGCCTATCACCGCGATGGCaa                                                                                                       >  7:355493/1‑58 (MQ=255)
                                                                                                                                       ttAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATGGCCTATCACCGCGATGGCaa                                                                                                       <  8:355493/58‑1 (MQ=255)
                                                                                                                                          aTGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGGCGCTTCCATGTCGTtg           <  3:114166/147‑1 (MQ=255)
                                                                                                                                             ccTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCgg                                                                               <  1:37384/76‑1 (MQ=255)
                                                                                                                                              cTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGtgaat      >  8:437545/1‑148 (MQ=255)
                                                                                                                                                ggTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGtgaatg     >  4:302537/1‑147 (MQ=255)
                                                                                                                                                ggTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGtgaatg     >  2:394531/1‑147 (MQ=255)
                                                                                                                                                ggTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGtga        >  7:55754/1‑144 (MQ=255)
                                                                                                                                                ggTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGtga        <  8:55754/144‑1 (MQ=255)
                                                                                                                                                  tttGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGTGAATGaaa  <  3:28025/148‑1 (MQ=255)
                                                                                                                                                  tttGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGTGAATGaaa  <  5:134878/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGTGAATGAAA  >  NC_000913/3507325‑3507618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: