Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,654,551 T→G 5.1% intergenic (+24/‑132) slp → / → dctR outer membrane lipoprotein/Putative LuxR family repressor for dicarboxylate transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,654,5510TG 5.1% 377.8 / 11.9 117intergenic (+24/‑132)slp/dctRouter membrane lipoprotein/Putative LuxR family repressor for dicarboxylate transport
Reads supporting (aligned to +/- strand):  ref base T (56/54);  new base G (2/4);  total (59/58)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.86e-01

Only 100 of 123 total aligned reads displayed.

GTTAATACCACCTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTTATAATTA  >  NC_000913/3654408‑3654698
                                                                                                                                               |                                                                                                                                                   
gTTAATACCACCTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGGTGCTTtct                                                                                                                                                  >  1:133152/1‑147 (MQ=255)
    aTACCACCTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCa                                                                                                                                             >  3:275945/1‑148 (MQ=255)
          ccTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTGAGAAAGCATCTGca                                                                                                                                       <  8:245200/148‑1 (MQ=255)
              taACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGcatc                                                                                                                                     <  3:785/146‑1 (MQ=255)
                aCCTGTGGGATTACGGCTTTGGTGCATTCTGGCCGTAACCGGGCTGGGGTTCGACTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGc                                                                                                                                 <  7:385399/148‑1 (MQ=255)
                aCCGGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGc                                                                                                                                 <  6:130987/148‑1 (MQ=255)
                  cTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCAt                                                                                                                               <  7:270136/148‑1 (MQ=255)
                   cgactcctggtgcggacattaactttataatcagtttgccaaaggtaattaacaggacagggcttataataataatagttagcttactgtgtattaatttgatgtaatgcatGATGCAGATGCTTTCTCAGCATc                                                                                                                                           >  4:318589‑M1/113‑135 (MQ=255)
                   cgactcctggtgcggacattaactttataatcagtttgccaaaggtaattaacaggacagggcttataataataatagttagcttactgtgtattaatttgatgtaatgcatGATGCAGATGCTTTCTCAGCATc                                                                                                                                           <  3:318589‑M1/23‑1 (MQ=255)
                    gtgGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTa                                                                                                                             >  8:437931/1‑148 (MQ=255)
                     tgGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATTCATTa                                                                                                                             <  2:49372/147‑1 (MQ=255)
                          ttACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCaa                                                                                                                       <  4:78910/148‑1 (MQ=255)
                              ggCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTa                                                                                                                   <  4:39380/148‑1 (MQ=255)
                              ggCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTa                                                                                                                   <  7:250060/148‑1 (MQ=255)
                               gCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTACATCATGCATTACATCAAATTaa                                                                                                                  <  2:253566/148‑1 (MQ=255)
                                   tGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATa                                                                                                                <  7:437931/146‑1 (MQ=255)
                                       gCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAg                                                                                                           >  5:345235/1‑147 (MQ=255)
                                           tCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAAtt                                                                                                                    <  8:432024/134‑1 (MQ=255)
                                           tCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAAtt                                                                                                                    >  7:432024/1‑134 (MQ=255)
                                           tCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGc                                                                                                      >  6:414826/1‑148 (MQ=255)
                                            cTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGc                                                                                                      >  2:178241/1‑147 (MQ=255)
                                              ggCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGGAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTaa                                                                                                   >  2:311100/1‑148 (MQ=255)
                                                  ggAACCCGGCTGGCGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATCCATTAAAACAAATAAATAAACAGTACGCAAACtat                                                                                               >  6:267957/1‑148 (MQ=255)
                                                    aaCCGGGCTGGGGTGCGCCTTACTACACCTATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACtatta                                                                                             <  2:328666/148‑1 (MQ=255)
                                                              gggTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATtata                                                                                     <  3:165860/146‑1 (MQ=255)
                                                                  gcgcCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATtata                                                                                     >  7:323226/1‑142 (MQ=255)
                                                                  gcgcCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATtata                                                                                     <  8:323226/142‑1 (MQ=255)
                                                                       ttACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTaa                                                                                                        <  4:181115/118‑1 (MQ=255)
                                                                       ttACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTaa                                                                                                        >  3:181115/1‑118 (MQ=255)
                                                                        tactacACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTa                                                                                                    <  1:109361/121‑1 (MQ=255)
                                                                        tactacACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACtattattatt                                                                                        >  2:208924/1‑133 (MQ=255)
                                                                        tactacACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCcctgtcctg                                                                         >  3:143941/1‑148 (MQ=255)
                                                                         actacACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCcctgtcctg                                                                         <  1:311100/147‑1 (MQ=255)
                                                                          ctacACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGtt                                                                       <  4:275945/148‑1 (MQ=255)
                                                                            acacCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACtattattatt                                                                                        <  1:370808/129‑1 (MQ=255)
                                                                            acacCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTaa                                                                     >  1:63401/1‑148 (MQ=255)
                                                                             cacCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCcctgt                                                                             <  3:240879/139‑1 (MQ=255)
                                                                             cacCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCcctgt                                                                             >  4:240879/1‑139 (MQ=255)
                                                                              acCAATGCTGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACtat                                                                                               <  7:449759/120‑1 (MQ=255)
                                                                              acCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACtat                                                                                               >  8:449759/1‑120 (MQ=255)
                                                                              acCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAAt                                                                    >  2:300097/1‑147 (MQ=255)
                                                                                 aaTGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACtattatt                                                                                           <  1:216321/121‑1 (MQ=255)
                                                                                 aaTGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTAAGTCAGCATCTGcatc                                                                                                                                     >  4:372865/1‑79 (MQ=255)
                                                                                    gCGGTGAGTCAGGTTACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACAGCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCttt                                                             >  4:74589/1‑148 (MQ=255)
                                                                                     cGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCttt                                                             <  1:300097/147‑1 (MQ=255)
                                                                                       gTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTgg                                                           >  3:125016/1‑147 (MQ=255)
                                                                                       ataataatagttagcttactgtgtattaatttgatgtaatgcatGATGCAGATGCTGAGAAAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAAt                                                                    <  8:441367‑M1/94‑1 (MQ=255)
                                                                                          aGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGGTGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCaaa                                                       <  2:133152/148‑1 (MQ=255)
                                                                                             cAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATac                                                                                                               >  6:56340/1‑89 (MQ=255)
                                                                                               ggTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGAt                                                  <  4:143941/148‑1 (MQ=255)
                                                                                                 tAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACtattat                                                                                            <  6:412657/104‑1 (MQ=255)
                                                                                                 tAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACtattat                                                                                            >  5:412657/1‑104 (MQ=255)
                                                                                                  aaCACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCAt                                                                                                                                            <  8:195065/55‑1 (MQ=255)
                                                                                                  aaCACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCAt                                                                                                                                            >  7:195065/1‑55 (MQ=255)
                                                                                                   acacCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGAATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATtata                                              <  5:414826/148‑1 (MQ=255)
                                                                                                    cacCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTg                                                    >  7:259317/1‑141 (MQ=255)
                                                                                                    cacCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATtata                                              >  7:133309/1‑147 (MQ=255)
                                                                                                    cacCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCGGcatca                                                                                                                                    >  6:294886/1‑61 (MQ=255)
                                                                                                     acCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATCCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAAAACACAGTAAGATAACAATTAATATTATAAGCACTGTCCTGTTAATTACCTTTGTCAAACTGataata                                              >  8:367421/1‑146 (MQ=255)
                                                                                                      ccTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTgg                                                           <  6:428110/132‑1 (MQ=255)
                                                                                                      ccTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTgg                                                           >  5:421690/1‑132 (MQ=255)
                                                                                                      ccTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTgg                                                           >  5:428110/1‑132 (MQ=255)
                                                                                                      ccTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAg                                           >  4:188798/1‑148 (MQ=255)
                                                                                                        tGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGt                                          >  4:168711/1‑147 (MQ=255)
                                                                                                         gAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGtt                                         >  5:137450/1‑147 (MQ=255)
                                                                                                          aGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTaa                                       <  4:117518/148‑1 (MQ=255)
                                                                                                          aGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTaa                                       <  6:137450/148‑1 (MQ=255)
                                                                                                              ggTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTc                                   >  7:204506/1‑148 (MQ=255)
                                                                                                               gTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTc                                   <  1:178241/147‑1 (MQ=255)
                                                                                                               attaatttgatgtaatgcatGATGCAGATGCTGAGAAAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAg                                                                                   >  7:408497‑M1/21‑99 (MQ=255)
                                                                                                               attaatttgatgtaatgcatGATGCAGATGCTGAGAAAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAg                                                                                   <  8:408497‑M1/79‑1 (MQ=255)
                                                                                                                tCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACATTTGGCAAACTGATTATAAAGTTAATGTCCg                                 >  1:261747/1‑148 (MQ=255)
                                                                                                                   aaTAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTaa                                       >  7:49479/1‑139 (MQ=255)
                                                                                                                     tAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCa                            <  8:204506/148‑1 (MQ=255)
                                                                                                                          cgacagAAAGATGCTGATGCTTTCTGAGCATCTTCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTTTTATTATAAGCCCTGTCGTGTTAATTACGTTTGTCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGt                       <  5:267957/143‑1 (MQ=255)
                                                                                                                          ccaccGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACAAGCAGt                       >  5:259156/1‑148 (MQ=255)
                                                                                                                           caccGAAAGATGCAGATGATTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTc                      >  6:95284/1‑148 (MQ=255)
                                                                                                                            accGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGa                                                   >  6:175148/1‑118 (MQ=255)
                                                                                                                            accGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATaaa                                            <  2:378563/125‑1 (MQ=255)
                                                                                                                            accGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATaaa                                            >  1:378563/1‑125 (MQ=255)
                                                                                                                              cGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGt                   >  8:397174/1‑148 (MQ=255)
                                                                                                                               gAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGtt                  <  2:63401/148‑1 (MQ=255)
                                                                                                                               gAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGt                   >  1:436583/1‑147 (MQ=255)
                                                                                                                                 aaGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTaa                                       <  5:104793/125‑1 (MQ=255)
                                                                                                                                 aaGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTaa                                       >  6:104793/1‑125 (MQ=255)
                                                                                                                                 aaGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTAt                <  4:125016/148‑1 (MQ=255)
                                                                                                                                    ATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGtt             <  1:152159‑M1/148‑1 (MQ=255)
                                                                                                                                    ATGCAGATGCTGAGAAAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGtt             <  1:11920‑M1/148‑1 (MQ=255)
                                                                                                                                     TGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGttt            >  2:152159‑M1/1‑148 (MQ=255)
                                                                                                                                       CAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCt          <  7:416083‑M1/148‑1 (MQ=255)
                                                                                                                                         GATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTta        <  7:425026‑M1/148‑1 (MQ=255)
                                                                                                                                         GATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAAAGTCCGCACCAGGAGTCGGTTATGTTTCTta        <  3:288171‑M1/148‑1 (MQ=255)
                                                                                                                                          ATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTta        <  7:335455‑M1/147‑1 (MQ=255)
                                                                                                                                           TGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTtat       <  3:66670‑M1/147‑1 (MQ=255)
                                                                                                                                               ttCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCt                                                               >  4:294492/1‑87 (MQ=255)
                                                                                                                                               ttCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCt                                                               <  3:294492/87‑1 (MQ=255)
                                                                                                                                               ttCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTa                 >  6:365968/1‑133 (MQ=255)
                                                                                                                                               ttCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTa                 <  5:365968/133‑1 (MQ=255)
                                                                                                                                               ttCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTTATAATTa  >  1:250563/1‑148 (MQ=255)
                                                                                                                                               ttCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTTATAATTa  >  3:191701/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
GTTAATACCACCTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTTATAATTA  >  NC_000913/3654408‑3654698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: