Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,287,672	C→T	intergenic (‑50/+110)	tyrT ← / ← purU	tRNA‑Tyr/formyltetrahydrofolate hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,287,672	0	C	T	100.0%	129.2 / NA	37	intergenic (‑50/+110)	tyrT/purU	tRNA‑Tyr/formyltetrahydrofolate hydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/21);  total (16/21)

TGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAA  >  NC_000913/1287526‑1287810                                                                                                                                                   |                                                                                                                                           tgtgGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGtat                                                                                                                                          >  5:107363/1‑149 (MQ=255)   tggtgAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGtatc                                                                                                                                         <  1:909/148‑1 (MQ=255)        aaTTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATc                                                                                                                                    <  8:61147/148‑1 (MQ=255)               gtggtggGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCCCCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGAcg                                                                                                                            >  6:97089/1‑149 (MQ=255)               gtggtggGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGAcg                                                                                                                            >  6:140852/1‑149 (MQ=255)               gtggtggGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGAcg                                                                                                                            >  6:143642/1‑149 (MQ=255)               gtggtggGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGAcg                                                                                                                            >  6:99968/1‑149 (MQ=255)                tggtggGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGAcgc                                                                                                                           <  6:107363/149‑1 (MQ=255)                  gtggGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACgcgc                                                                                                                         >  7:106865/1‑149 (MQ=255)                    gggggAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGcgccg                                                                                                                       >  3:16035/1‑149 (MQ=255)                       ggAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTg                                                                                                                    >  1:73940/1‑149 (MQ=255)                             aTTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGtatcat                                                                                                                                       <  6:28179/124‑1 (MQ=255)                             aTTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGtatcat                                                                                                                                       >  5:28179/1‑124 (MQ=255)                             aTTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACgcgc                                                                                                                         >  3:68822/1‑138 (MQ=255)                             aTTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACgcgc                                                                                                                         <  4:68822/138‑1 (MQ=255)                             aTTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAgt                                                                                                              <  2:111398/149‑1 (MQ=255)                                 gAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTa                                                                                                          <  2:73940/149‑1 (MQ=255)                                    ccTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACTGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGAc                                                                                                                             <  8:16875/127‑1 (MQ=255)                                    ccTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGAc                                                                                                                             >  7:16875/1‑127 (MQ=255)                                     cTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGtatc                                                                                                                                         <  3:48624/114‑1 (MQ=255)                                     cTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGtatc                                                                                                                                         >  4:48624/1‑114 (MQ=255)                                                 tGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGaa                                                                                          <  2:108659/149‑1 (MQ=255)                                                   aCGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAAc                                                                                         <  4:16035/148‑1 (MQ=255)                                                     ggCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTgg                                                                                      <  5:99968/149‑1 (MQ=255)                                                     ggCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTgg                                                                                      <  5:97089/149‑1 (MQ=255)                                                       cAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGtt                                                                                    <  1:16646/149‑1 (MQ=255)                                                                         ccTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAgt                                                                                                              >  4:17773/1‑105 (MQ=255)                                                                         ccTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAgt                                                                                                              <  3:17773/105‑1 (MQ=255)                                                                             tGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACg                                                               <  8:106865/148‑1 (MQ=255)                                                                              ggCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACgg                                                              <  5:143642/148‑1 (MQ=255)                                                                              ggCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACgg                                                              <  5:140852/148‑1 (MQ=255)                                                                                                   cGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAAc                                                  >  5:148420/1‑139 (MQ=255)                                                                                                   cGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAAc                                                  <  6:148420/139‑1 (MQ=255)                                                                                                       gTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCa                                                                        >  8:63480/1‑113 (MQ=255)                                                                                                       gTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCa                                                                        <  7:63480/113‑1 (MQ=255)                                                                                                                       gCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTAtttt                                        <  1:52102/129‑1 (MQ=255)                                                                                                                       gCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTAtttt                                        >  2:52102/1‑129 (MQ=255)                                                                                                                         cTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGa                               <  2:43366/136‑1 (MQ=255)                                                                                                                         cTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGa                               >  1:43366/1‑136 (MQ=255)                                                                                                                             tCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTc              >  3:101770/1‑149 (MQ=255)                                                                                                                                cTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGAt           >  1:11851/1‑149 (MQ=255)                                                                                                                                         aaGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTaaa  <  2:11851/149‑1 (MQ=255)                                                                                                                                         aaGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTaaa  <  4:101770/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                           TGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAA  >  NC_000913/1287526‑1287810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑