Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 891,882 C→G 5.8% H233Q (CAC→CAG)  nfsA → nitroreductase A, NADPH‑dependent, FMN‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913891,8820CG 5.8% 282.2 / 6.1 83H233Q (CAC→CAG) nfsAnitroreductase A, NADPH‑dependent, FMN‑dependent
Reads supporting (aligned to +/- strand):  ref base C (43/35);  new base G (2/3);  total (45/38)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATAAAGGCGCACTGGCGCAGTATGACGAGCAACTGGCGGAATATTACCTCACCCGTGGCAGCAATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCC  >  NC_000913/891736‑892029
                                                                                                                                                  |                                                                                                                                                   
gATAAAGGCGCACTGGCGCAGTATGACGAGCAACTGGCGGAATATTACCTCACCCGTGGCAGCAATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGcaca                                                                                                                                                    >  3:9761/1‑148 (MQ=255)
 aTAAAGGCGCACTGGCGCAGTATGACGAGCAACTGGCGGAATATTACCTCACCCGTGGCAGCAATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACaaa                                                                                                                                                  <  8:49215/149‑1 (MQ=255)
     aGGCGCACTGGCGCAGTATGACGAGCAACTGGCGGAATATTACCTCACCCGTGGCAGCAATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGACCATTTATTCTGGAGTATTTGCACAAACAgg                                                                                                                                              >  2:88460/1‑149 (MQ=255)
         gcACTGGCGCAGTATGACGAGCAACTGGCGGAATATTACCTCACCCGTGGCAGCAATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTg                                                                                                                                          <  4:9761/149‑1 (MQ=255)
             tGGCGCAGTATGACGAGCAACTGGCGGAATATTACCTCACCCGTGGCAGCAATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACa                                                                                                                                                <  2:67829/139‑1 (MQ=255)
             tGGCGCAGTATGACGAGCAACTGGCGGAATATTACCTCACCCGTGGCAGCAATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACa                                                                                                                                                >  1:67829/1‑139 (MQ=255)
             tGGCGCAGTATGACGAGCAACTGGCGGAATATTACCTCACCCGTGGCAGCAATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCg                                                                                                                                      >  5:404550/1‑149 (MQ=255)
                   aGTATGACGAGCAACTGGCGGAATATTACCTCACCCGTGGCAGCAATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACgcgc                                                                                                                                <  5:295549/149‑1 (MQ=255)
                    gTATGACGAGCAACTGGCGGAATATTACCTCACCCGTGGCAGCAATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCt                                                                                                                               <  6:404550/149‑1 (MQ=255)
                     tATGACGAGCAACTGGCGGAATATTACCTCACCCGTGGCAGCAATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCt                                                                                                                               >  4:168315/1‑148 (MQ=255)
                     tATGACGAGCAACTGGCGGAATATTACCTCACCCGTGGCAGCAATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCt                                                                                                                               <  3:168315/148‑1 (MQ=255)
                      aTGACGAGCAACTGGCGGAATATTACCTCACCCGTGGCAGCAATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTa                                                                                                                              >  8:363294/1‑148 (MQ=255)
                       tGACGAGCAACTGGAGGAATATTACCTCACCCGTGGCAGCAATAATCGCCGGGATACCTGGAGCGATCATATCCGACGAACAATCATTAAAGAAAGCCGCCCATTTATTATGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTaa                                                                                                                             >  5:320829/1‑148 (MQ=255)
                        gACGAGCAACTGGCGGAATATTACCTCACCCGTGGCAGCAATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTaaaa                                                                                                                           >  5:263427/1‑149 (MQ=255)
                               aaCTGGCGGAATATTACCTCACCCGTGGCAGCAATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCAc                                                                                                                    >  2:326514/1‑149 (MQ=255)
                                    gCGGAATATTACCTCACCCGTGGCAGCAATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCg                                                                                                                <  2:209332/148‑1 (MQ=255)
                                      ggAATATTACCTCACCCGTGGCAGCAATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATg                                                                                                             <  6:185708/149‑1 (MQ=255)
                                      ggAATATTACCTCACCCGTGGCAGCAATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATg                                                                                                             >  8:364777/1‑149 (MQ=255)
                                              aCCTCACCCGTGGCAGCAATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATa                                                                                                      <  1:326514/148‑1 (MQ=255)
                                                cTCACCCGTGGCAGCAATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATAcgc                                                                                                   >  3:135872/1‑149 (MQ=255)
                                                   aCCCGTGGCAGCAATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCgg                                                                                                 <  7:345854/148‑1 (MQ=255)
                                                      cGTGGCAGCAATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCtt                                                                                             <  4:135872/149‑1 (MQ=255)
                                                          gcagcaATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAAc                                                                                                                          >  2:16098/1‑116 (MQ=255)
                                                          gcagcaATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAAc                                                                                                                          <  1:16098/116‑1 (MQ=255)
                                                          gcagcaATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGa                                                                                         <  3:16070/149‑1 (MQ=255)
                                                          gcagcaATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGAGTATTTGCACAAACACGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGa                                                                                         <  3:87091/149‑1 (MQ=255)
                                                             gcaATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCAGTTATTCTGGATTATTTGCCCAAACAGGGTTGGGCGACGCGCTAAAACCGCCCCGTCGATGTATGATACGCGGGCTTTTgcc                                                                                        >  1:101860/1‑145 (MQ=255)
                                                              caATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACAGCCACGTCGATGTATGATACGCGGGCTTTTGACCAg                                                                                     >  1:335862/1‑149 (MQ=255)
                                                               aataatCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGa                                                                                         <  8:291214/144‑1 (MQ=255)
                                                               aataatCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGa                                                                                         >  7:291214/1‑144 (MQ=255)
                                                               aataatCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAgg                                                                                    >  3:347350/1‑149 (MQ=255)
                                                                 taatCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGc                                                                                                                       >  3:298464/1‑112 (MQ=255)
                                                                 taatCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGc                                                                                                                       <  4:298464/112‑1 (MQ=255)
                                                                 taatCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTc                                                                                  >  6:24889/1‑149 (MQ=255)
                                                                 taatCGCCGGGATACCTGGAGCGATCATATCCGCAGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTc                                                                                  >  2:78816/1‑149 (MQ=255)
                                                                  aatCGCCGGGATACCTGGAGCGATCATATCCGCCGACCAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTa                                                                                                           >  1:323467/1‑123 (MQ=255)
                                                                  aatCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTa                                                                                                           <  2:323467/123‑1 (MQ=255)
                                                                      gCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGa                                                                               >  3:339113/1‑147 (MQ=255)
                                                                        cGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACa                                                                             >  7:3354/1‑147 (MQ=255)
                                                                                cTGGAGCGATCATATCCGCCTAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGAt                                                                                                       <  2:51993/113‑1 (MQ=255)
                                                                                cTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGAt                                                                                                       >  1:51993/1‑113 (MQ=255)
                                                                                cTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACagagag                                                                        <  2:291312/144‑1 (MQ=255)
                                                                                cTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACagagag                                                                        >  1:291312/1‑144 (MQ=255)
                                                                                cTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCAGCCCATTTATTCTGGATTATTAGCACAAACAGGGTTGGGCGACGCGCTAACACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTg                                                                     >  1:88440/1‑147 (MQ=255)
                                                                                 tGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGa                                                                                         <  1:224579/126‑1 (MQ=255)
                                                                                 tGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGa                                                                                         >  2:224579/1‑126 (MQ=255)
                                                                                   gAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAgg                                                                 <  6:263427/148‑1 (MQ=255)
                                                                                   gAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAgg                                                                 <  5:24889/148‑1 (MQ=255)
                                                                                      cGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTg                                                              >  8:37881/1‑148 (MQ=255)
                                                                                      cGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGa                                                             <  7:363294/149‑1 (MQ=255)
                                                                                       taTCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGa                                                             >  6:319559/2‑148 (MQ=255)
                                                                                        aTCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGa                                                                                                               <  7:241102/97‑1 (MQ=255)
                                                                                        aTCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGa                                                                                                               >  8:241102/1‑97 (MQ=255)
                                                                                         tCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCAGAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGaa                                                            <  7:240240/147‑1 (MQ=255)
                                                                                         tCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCAGAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGaa                                                            <  7:233984/147‑1 (MQ=255)
                                                                                         tCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCAGAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGaa                                                            >  8:240240/1‑147 (MQ=255)
                                                                                         tCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCAGAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGaa                                                            >  8:233984/1‑147 (MQ=255)
                                                                                         tCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGaaaa                                                          <  7:37881/149‑1 (MQ=255)
                                                                                           atatCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTCTTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAAtt                                                        <  4:339113/149‑1 (MQ=255)
                                                                                                 gccgAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGa                                                                               <  2:302892/120‑1 (MQ=255)
                                                                                                 gccgAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCata                                                  <  4:347350/149‑1 (MQ=255)
                                                                                                 gccgAACAATCATTAAAGAAAGCAGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGa                                                                               >  1:302892/1‑120 (MQ=255)
                                                                                                  ccgAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCatat                                                 >  6:101804/1‑149 (MQ=255)
                                                                                                  ccgAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCatat                                                 >  6:159058/1‑149 (MQ=255)
                                                                                                        aaTCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTccc                                           >  1:46884/1‑149 (MQ=255)
                                                                                                                   aaagCCGCACATTTATTCTCGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTAAGATACGCGGGCATTTGACCAGGTCTGACAGAGAGGTCCAGGGTGAAAATTGCCATATTGTCCAGGGATGGAAct                                >  8:116854/1‑148 (MQ=255)
                                                                                                                     agCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGGTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGct                              <  7:371493/149‑1 (MQ=255)
                                                                                                                           gcATTTATTCTGGATTATTTGCAGAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTc                        <  7:116854/148‑1 (MQ=255)
                                                                                                                           ccATTTATTCTGGATTATTTGCACAAACAGGTTTGTGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGGGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTc                        <  5:6187/149‑1 (MQ=255)
                                                                                                                           ccATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTc                        >  1:345174/1‑149 (MQ=255)
                                                                                                                             aTTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCgt                      >  4:350531/1‑149 (MQ=255)
                                                                                                                             aTTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCgt                      >  5:59054/1‑149 (MQ=255)
                                                                                                                             aTTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCgt                      >  2:156653/1‑149 (MQ=255)
                                                                                                                               ttATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCgtg                     >  5:313774/1‑148 (MQ=255)
                                                                                                                                  ttCTGGATTATTTGCACAAACAGGGGTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTaa                  <  1:88460/148‑1 (MQ=255)
                                                                                                                                    cTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGc                <  7:364777/148‑1 (MQ=255)
                                                                                                                                     tGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCgg              >  1:103634/1‑149 (MQ=255)
                                                                                                                                       gATTTTTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTGGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCt            >  2:384192/1‑149 (MQ=255)
                                                                                                                                       gATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGAt                                      <  8:182678/123‑1 (MQ=255)
                                                                                                                                       gATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGAt                                      >  7:182678/1‑123 (MQ=255)
                                                                                                                                         ttattTGCACAAACAGGGTTGGGCGACGCGCTAAAACAGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTTTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGc          >  5:291142/1‑149 (MQ=255)
                                                                                                                                           attTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCg         <  2:103634/148‑1 (MQ=255)
                                                                                                                                            tttGCACAAACAGGGTTGGGCGACGCGCTAATACCGCCACGTCGATGTTTGATACGCTGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGAGGGAACGCTCTATTCGGGTAAGCGGCttcac        <  6:320829/148‑5 (MQ=255)
                                                                                                                                             ttGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTg       >  3:232570/1‑148 (MQ=255)
                                                                                                                                                 acaAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGTCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGcc  >  2:160968/1‑149 (MQ=255)
                                                                                                                                                 acaAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGcc  >  2:34319/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GATAAAGGCGCACTGGCGCAGTATGACGAGCAACTGGCGGAATATTACCTCACCCGTGGCAGCAATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCC  >  NC_000913/891736‑892029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑