Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,345,632 T→C 6.3% T371A (ACG→GCG)  gmr ← cyclic‑di‑GMP phosphodiesterase; csgD regulator; modulator of RNase II stability

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,345,6320TC 6.3% 188.0 / 3.6 63T371A (ACG→GCG) gmrcyclic‑di‑GMP phosphodiesterase; csgD regulator; modulator of RNase II stability
Reads supporting (aligned to +/- strand):  ref base T (25/34);  new base C (2/2);  total (27/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.20e-01

TCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGTATAAACTTCAATTAAACCAATGCGAAAGGGGAGCCGTAAGCGGGTCAAAATTCGTGATGCCATTGCTTCCAGCGCGCTTTGTGAGGTGTTGGA  >  NC_000913/1345484‑1345776
                                                                                                                                                    |                                                                                                                                                
tCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGt                                                                                                                                                  >  5:290360/1‑149 (MQ=255)
         aGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGa                                                                                                                                         <  5:25871/149‑1 (MQ=255)
         aGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGa                                                                                                                                         <  1:419575/149‑1 (MQ=255)
            gCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAAcc                                                                                                                                      <  2:372680/149‑1 (MQ=255)
                  cGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCTTTGATTCATTTCTGGTGTAAAACCGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTc                                                                                                                                <  1:416520/149‑1 (MQ=255)
                    cAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGTGTAAAAACGCAACATGGTCCTCGTCCGCCTTCCTGCGCTGTGGACATTGCTGTGTCGGCGGGACAAATAATAGCCGTGCTGTCTGAACCATGTTCgg                                                                                                                              <  7:295515/149‑1 (MQ=255)
                           gTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGgagaga                                                                                                                        <  6:375012/148‑1 (MQ=255)
                              tCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAACTTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTg                                                                                                                     <  3:139379/148‑1 (MQ=255)
                                cagccagAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCaa                                                                                                                  >  5:328895/1‑149 (MQ=255)
                                 agccagAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCgg                                                                                                                              <  2:230107/136‑1 (MQ=255)
                                 agccagAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCgg                                                                                                                              >  1:230107/1‑136 (MQ=255)
                                     agagATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATAcc                                                                                                              >  6:342569/1‑148 (MQ=255)
                                      gagaTATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTa                                                                                                            <  4:159355/149‑1 (MQ=255)
                                           atTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGaa                                                                                                       >  4:283111/1‑149 (MQ=255)
                                           atTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGCGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGa                                                                                                        >  7:113018/1‑148 (MQ=255)
                                           atTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGACCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGaa                                                                                                       >  2:199323/1‑149 (MQ=255)
                                            ttCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGCGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAAc                                                                                                      <  8:113018/149‑1 (MQ=255)
                                             tCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGaa                                                                                                       >  6:68516/1‑147 (MQ=255)
                                               aaaTACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGc                                                                                                   >  3:228902/1‑149 (MQ=255)
                                                aaTACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACAATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCt                                                                                                  >  4:70219/1‑149 (MQ=255)
                                                 aTACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTg                                                                                                 <  6:226817/149‑1 (MQ=255)
                                                 aTACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTg                                                                                                 <  5:342569/149‑1 (MQ=255)
                                                  tACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTgg                                                                                                <  6:328895/149‑1 (MQ=255)
                                                    cccGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACACTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGta                                                                                              <  5:351700/149‑1 (MQ=255)
                                                       gTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGga                                                                                                                            <  2:393185/116‑1 (MQ=255)
                                                       gTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGga                                                                                                                            >  1:393185/1‑116 (MQ=255)
                                                            ttCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGTATAAACtt                                                                                       >  6:243227/1‑148 (MQ=255)
                                                            ttCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGCGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTg                                                                                                 >  5:61960/1‑138 (MQ=255)
                                                            ttCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGCGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTg                                                                                                 <  6:61960/138‑1 (MQ=255)
                                                             tCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTg                                                                                                                                                 >  4:330601/1‑89 (MQ=255)
                                                             tCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTg                                                                                                                                                 <  3:330601/89‑1 (MQ=255)
                                                               aTTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGt                                                                                               <  6:136747/137‑1 (MQ=255)
                                                               aTTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGt                                                                                               >  5:136747/1‑137 (MQ=255)
                                                                tttCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGtt                                                                                                                                 <  1:173487/102‑1 (MQ=255)
                                                                tttCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGAGTGACGAATAATAGCCGTGCTGTCTGAACCATGtt                                                                                                                                 >  2:173487/1‑102 (MQ=255)
                                                                      gggTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGTATAAACTTCAATTaaa                                                                               <  3:392324/146‑1 (MQ=255)
                                                                      gggTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGTATAAACTTCAATTAAAc                                                                              <  4:371078/147‑1 (MQ=255)
                                                                      gggTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGTATAAACTTCAATTAAAc                                                                              <  6:290360/147‑1 (MQ=255)
                                                                      gggTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGTATAAACTTCAATTAAAc                                                                              <  3:283111/147‑1 (MQ=255)
                                                                      gggTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCGGTGTCGGCGTGACGAATAATAGCCGTGCTCTCTGAACCATGTTCGGGAGAGAGTGCAAAACCTACTGAACAGCTGGTATAAACTTCAATTAAAcc                                                                             >  2:380608/1‑148 (MQ=255)
                                                                       ggTAAAAACCCAAAATTGTCATCTTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGCCGTGACGAAAAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGTATAAACTTCAATTAAACCaa                                                                           >  4:391656/1‑149 (MQ=255)
                                                                           aaaaCGCAAAATTGTCATCGTCCGCCATACTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGTATAAACTTCAATTAAACCAATGc                                                                        >  6:393400/1‑148 (MQ=255)
                                                                                  aaaaTTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGTATAAACTTCaa                                                                                    <  6:80111/129‑1 (MQ=255)
                                                                                  aaaaTTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGTATAAACTTCaa                                                                                    >  5:80111/1‑129 (MQ=255)
                                                                                     aTTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGTATAAACTTCAATTAAACCAATGCGAAAGGGGAGc                                                             >  1:408406/1‑149 (MQ=255)
                                                                                            tCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGTATAAACTTCTATAAAACCAATGCGAAAGGGGAGCCGTAAGc                                                      >  5:141498/1‑149 (MQ=255)
                                                                                              gTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGTATAAACTTCAATTAAACCAATGCGAAAGGGGAGCCGTAAGCgg                                                    >  2:242312/1‑149 (MQ=255)
                                                                                                           cGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGTATAAACTTCAATTAAACCAATGCGAAAGGGGAGCCGTAAGCGGGTCAAAATTCGt                                        <  5:243227/148‑1 (MQ=255)
                                                                                                             cTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGTATAAACTTCAATTAAACCAATGCGAAAGGGGAGCCGTAAGCGGGTCAAAATTCGTGAt                                     <  5:393400/149‑1 (MQ=255)
                                                                                                                tgtACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGTATAAACTTCAATTAAACCAATGCGAAAGGGGAGCCGTAAGCGGGTCAAAATTCGTGATGc                                   >  8:353287/1‑148 (MQ=255)
                                                                                                                    cATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGTATAAACTTCAATTAAACCAATGCGAAAGGGGAGCCGTAAGCGGGTCAAAATTCGTGATGCCATTg                              >  4:58531/1‑149 (MQ=255)
                                                                                                                     aTTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGTATAAACTTCAATTAAACCAATGCGAAAGGGGAGCCGTAAGCgg                                                    >  2:237537/1‑126 (MQ=255)
                                                                                                                     aTTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGTATAAACTTCAATTAAACCAATGCGAAAGGGGAGCCGTAAGCgg                                                    <  1:237537/126‑1 (MQ=255)
                                                                                                                      ttGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGTATAAACTTCAATTAAACCAATGCGAAAGGGGAGCCGTAAGCGGGTCAAAATTCGTGATGCCATTGCt                            >  8:315783/1‑149 (MQ=255)
                                                                                                                         cTGTGTCGGCGTGACGAATAATAGCCGTGCAGTCTGTACCATGTTCGGGAGAGAGGGCAATACCTACTGAACAGCTGGTATAAACTTCAATTAAACCAATGCGAAAGGGGAGCCGTAAGCGGGGCAAAATTCGTGATGCCATTGCTTc                          <  2:100344/148‑1 (MQ=255)
                                                                                                                            tgtCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGTATAAACTTCAATTAAACCAATGCGAAAGGGGAGCCGTAAGCGGGTCAAAATTCGTGATGCCATTGCTTCCAgc                      <  3:376617/149‑1 (MQ=255)
                                                                                                                                   gTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGTATAAACTTCAATTAAACCAATGCGAAAGGGGAGCCGTAAGCGGGTCAAAATTCGTGATGCCATTGCTTCCAGCGCGCttt               <  1:85785/149‑1 (MQ=255)
                                                                                                                                        gAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGTATAAACTTCAATTAAACCAATGCGAAAGGGGAGCCGTAAGCGGGTCAAAATTCGTGATGCCATTGCTTCCAGCGCGCTTTGTGAg          <  1:146876/149‑1 (MQ=255)
                                                                                                                                              taGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGTATAAACTTCAATTAAACCAATGCGAAAGGGGAGCCGTAAGCGGGTCAAAATTCGTGATGCCATTGCTTCCAGCGCGCTTTGTGAGGTGTTg    <  3:58531/149‑1 (MQ=255)
                                                                                                                                              taGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGTATAAACTTCAATTAAACCAATGCGAAAGGGGAGCCGTAAGCGGGTCAAAATTCGTGATGCCATTGCTTCCAGCGCGCTTTGTGAGGTGTTg    <  5:68516/149‑1 (MQ=255)
                                                                                                                                               aGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGTATAAACTTCAATTAAACCAATGCGAAAGGGGAGCCGTAAGCGGGTCAAAATTCGTGATGCCATTGCTTCCAGCGCGCTTTGTGAGGTGTTg    >  4:388881/1‑148 (MQ=255)
                                                                                                                                                gCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGTATAAACTTCAATTAAACCAATGCGAAAGGGGAGCCGTAAGCGGGTCAAAATTCGTGATGCCATTGCTTCCAGCGCGCTTTGTGAGGTGTTGGa  <  1:242312/149‑1 (MQ=255)
                                                                                                                                                gCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGTATAAACTTCAATTAAACCAATGCGAAAGGGGAGCCGTAAGCGGGTCAAAATTCGTGATGCCATTGCTTCCAGCGCGCTTTGTGAGGTGTTGGa  <  7:284828/149‑1 (MQ=255)
                                                                                                                                                gCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGTATAAACTTAAATTAAACCAATGCGAAAGGGGAGCCGTAAGCGGGTCAAAATTCGTGATGCCATTGCTTCCAGCGCGCTTTGTGAGGTGTTgg   <  1:199323/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
TCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGCCGTGCTGTCTGAACCATGTTCGGGAGAGAGTGCAATACCTACTGAACAGCTGGTATAAACTTCAATTAAACCAATGCGAAAGGGGAGCCGTAAGCGGGTCAAAATTCGTGATGCCATTGCTTCCAGCGCGCTTTGTGAGGTGTTGGA  >  NC_000913/1345484‑1345776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑