Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,352,369 C→T 5.0% R89H (CGT→CAT)  sapF ← antimicrobial peptide ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,352,3690CT 5.0% 259.2 / 2.4 79R89H (CGT→CAT) sapFantimicrobial peptide ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (41/34);  new base T (2/2);  total (43/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01

CCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCATTCCCGCCAGCATTTTTGCCAGCGTGGATTTACCCGAACCATTCTCGCCAATAATCGCCAGTGTCTG  >  NC_000913/1352226‑1352514
                                                                                                                                               |                                                                                                                                                 
ccATACGCATCTTTTCAATAATCTGTTTGCGAAGCTGTTCCTGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAACTCATGCGAATACGCTGa                                                                                                                                              <  8:412530/149‑1 (MQ=255)
 cATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGAc                                                                                                                                             >  2:200602/1‑149 (MQ=255)
  aTACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACt                                                                                                                                            >  2:15151/1‑149 (MQ=255)
     cGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCg                                                                                                                                         >  4:185281/1‑149 (MQ=255)
         tCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACg                                                                                                                                                  <  6:384887/136‑1 (MQ=255)
         tCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACg                                                                                                                                                  >  5:384887/1‑136 (MQ=255)
         tCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCggaag                                                                                                                                     >  4:202948/1‑149 (MQ=255)
            tttCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCggaaggaa                                                                                                                                  >  5:63344/1‑149 (MQ=255)
            tttCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCggaaggaa                                                                                                                                  >  4:300242/1‑149 (MQ=255)
             ttCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCggaag                                                                                                                                     >  2:62474/1‑145 (MQ=255)
             ttCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCggaag                                                                                                                                     <  1:62474/145‑1 (MQ=255)
                    atCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGc                                                                                                                           <  3:300242/148‑1 (MQ=255)
                       tGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCaa                                                                                                                        >  2:369855/1‑148 (MQ=255)
                             cGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAg                                                                                                                 <  1:160834/149‑1 (MQ=255)
                              gACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGt                                                                                                                >  7:45513/1‑149 (MQ=255)
                                    gTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAACATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGgatga                                                                                                          >  1:292461/1‑149 (MQ=255)
                                               aGGTCGGAGTTCACGCGCAGTGGAAAATCCAGAATTAGCCAGATACGTTGACGGGGATTCAACGAGCACGAAGGATCCTGAAAAATCAACCCAATACGCTGACTGCGGAAGGAATACTCGCCAAAATGCAGTGGATGCTCGtcaatcc                                                                                                >  8:241419/1‑147 (MQ=255)
                                                 gTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATa                                                                                             <  3:202948/149‑1 (MQ=255)
                                                 gTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATa                                                                                             <  4:41167/149‑1 (MQ=255)
                                                 gTCGGTGCTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGGTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATCCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATa                                                                                             <  6:157522/149‑1 (MQ=255)
                                                      tgtTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTcg                                                                                        >  1:212116/1‑149 (MQ=255)
                                                            ggCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTg                                                                                  >  7:273680/1‑149 (MQ=255)
                                                             gcgcAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTgg                                                                                 >  6:75416/1‑149 (MQ=255)
                                                             gcgcAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGAAGTGGATGATCGTCAATCAATAACTCGCCGCTgg                                                                                 >  8:302667/1‑149 (MQ=255)
                                                               gcAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTa                                                                               <  1:369855/149‑1 (MQ=255)
                                                                 aGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGtcaatcaa                                                                                               <  8:28591/131‑1 (MQ=255)
                                                                 aGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGtcaatcaa                                                                                               >  7:28591/1‑131 (MQ=255)
                                                                   tGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAGCGAGGGCGAAGGATCCTGAAAATTCATGCGAATACGCGGACTGCGGAAGGAATAATCGCGAAACTGCAGGGGAGGATCGGCAAGCAATAACTCGCCGCTGGTAGGct                                                                           >  8:116316/1‑149 (MQ=255)
                                                                      aaaaTCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTa                                                                        >  6:368372/1‑149 (MQ=255)
                                                                       aaaTCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTa                                                                        <  5:75416/148‑1 (MQ=255)
                                                                       aaaTCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTAt                                                                       >  7:65214/1‑149 (MQ=255)
                                                                         aTCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCa                                                                     <  8:65214/149‑1 (MQ=255)
                                                                           ccAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCAtt                                                                   >  7:427899/1‑149 (MQ=255)
                                                                            cAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGTATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCAtt                                                                   <  5:136515/148‑1 (MQ=255)
                                                                            cAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCAtt                                                                   <  5:368372/148‑1 (MQ=255)
                                                                            cAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCATTc                                                                  >  8:118537/1‑149 (MQ=255)
                                                                            cAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCATTc                                                                  >  8:49697/1‑149 (MQ=255)
                                                                            cAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCATTc                                                                  >  7:178105/1‑149 (MQ=255)
                                                                             aggaTTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCATTcc                                                                 >  5:128211/4‑149 (MQ=255)
                                                                               aaTTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGc                                                                                                                                                 <  1:198123/67‑1 (MQ=255)
                                                                               aaTTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGc                                                                                                                                                 >  2:198123/1‑67 (MQ=255)
                                                                                   tGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCATTCCCGCCa                                                            <  3:185281/148‑1 (MQ=255)
                                                                                        gaTACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCATTCCCGCCAGCAttt                                                      >  5:342648/1‑149 (MQ=255)
                                                                                          tACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGgatgat                                                                                                         >  8:222140/1‑96 (MQ=255)
                                                                                          tACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGgatgat                                                                                                         <  7:222140/96‑1 (MQ=255)
                                                                                             gTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCATTCCCGCCAGCATTTTTGcc                                                 <  6:63344/149‑1 (MQ=255)
                                                                                                 aCGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCATTCCCGCCAGCATTTTTGCCAGc                                              <  8:125704/148‑1 (MQ=255)
                                                                                                  cGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCATTCCCGCCAGCATTTTTGCCAGCGt                                            >  7:20195/1‑149 (MQ=255)
                                                                                                     ggATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCATTCCCGCCAGCATTTTTGCCAGCGTGGa                                         <  1:15151/149‑1 (MQ=255)
                                                                                                         tCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCATTCCCGCCAGCATTTTTGCCAGCGTGGATTTa                                     >  2:26261/1‑149 (MQ=255)
                                                                                                         tCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCATTCCCGCCAGCATTTTTGCCAGCGTGGATTTa                                     >  8:197808/1‑149 (MQ=255)
                                                                                                         tCAACGACGTCGAAGGATCCTGAAAAATCATGCGAATACTCTGCCTGCCGAAGCAATAATCGCCAAAATGCAGTCGATTAACGTCAATAAATAACTCGCCGATGGTAGGCTCAATCATTCCCGCCATCATATTTGCCAGCGTgctttaa                                     >  5:247641/1‑143 (MQ=255)
                                                                                                           aaCGATGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAAGCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCATTCCCGCCAGCATTTTTGCCAGCGTGGATTTAc                                    <  1:335603/148‑1 (MQ=255)
                                                                                                           aaCGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCTTAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTgg                                                                                 <  7:296085/103‑1 (MQ=255)
                                                                                                           aaCGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTgg                                                                                 >  8:296085/1‑103 (MQ=255)
                                                                                                           aaCGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCATTCCCGCCAGCATTTTTGCCAGCGTGGATTTAcc                                   >  1:9154/1‑149 (MQ=255)
                                                                                                            aCGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGcc                                                                                                                          >  4:28990/1‑61 (MQ=255)
                                                                                                            aCGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGcc                                                                                                                          <  3:28990/61‑1 (MQ=255)
                                                                                                             cGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCATTCCCGCCAGCATTTTTGCCAGCGTGGATTTAccc                                  >  3:310677/1‑148 (MQ=255)
                                                                                                               aGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCATTCCCGCCAGCATTTTTGCCAGCGTGGATTTACCCGaa                               <  2:212116/149‑1 (MQ=255)
                                                                                                               aGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCATTCCCGCCAGCATTTTTGCCAGCGTGGATTTACCCGaa                               >  7:372412/1‑149 (MQ=255)
                                                                                                                ggTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCATTCCCGCCAGCATTTTTGCCAGCGTGGATTTACCCGAAc                              <  7:118537/149‑1 (MQ=255)
                                                                                                                 gTCGAAGGATCCTGAAAAATCATGCGAATATGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGt                                                                                                                <  6:394379/66‑1 (MQ=255)
                                                                                                                 gTCGAAGGATCCTGAAAAATCATGCGAATATGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGt                                                                                                                >  3:8315/1‑66 (MQ=255)
                                                                                                                 gTCGAAGGATCCTGAAAAATCATGCGAATATGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGt                                                                                                                <  4:8315/66‑1 (MQ=255)
                                                                                                                 gTCGAAGGATCCTGAAAAATCATGCGAATATGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGt                                                                                                                >  5:394379/1‑66 (MQ=255)
                                                                                                                    gAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATaa                                                                                            >  3:230536/1‑83 (MQ=255)
                                                                                                                    gAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATaa                                                                                            <  4:230536/83‑1 (MQ=255)
                                                                                                                     aaGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGCTCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCATTCCCGCCAGCATTTTTGCCAGCGTGGATTTACCCGAACCATtc                         >  5:84182/1‑149 (MQ=255)
                                                                                                                     aaGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCATTCCCGCCAGCATTTTTGCCAGCGTGGATTTACCCGAACCATtc                         >  8:46822/1‑149 (MQ=255)
                                                                                                                     aaGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAAGCAATAACTCGCCGCTGGGAGGCTCTATCATTCCCGCCAGCATTTTTGCCAGCGTGGATTTACCCGAACCATtc                         <  6:332306/149‑1 (MQ=255)
                                                                                                                       ggATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCATTCCCGCCAGCATTTTTGCCAGCGTGGATTTACCCGAACCATtctc                       <  8:372412/149‑1 (MQ=255)
                                                                                                                       ggATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCATTCCCGCCAGCATTTTTGCCAGCGTGGATTTACCCGAACCATtctc                       <  8:45513/149‑1 (MQ=255)
                                                                                                                         aTCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTgg                                                                                 >  2:117215/1‑89 (MQ=255)
                                                                                                                         aTCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTgg                                                                                 <  1:117215/89‑1 (MQ=255)
                                                                                                                                      aTGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCATTCCCGCCAGCATTTTTGCCAGCGTGGATTTACCCGAACCATTCTCGCCAATAATCGCCa         <  8:20195/148‑1 (MQ=255)
                                                                                                                                      aTGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCATTCCCGCCAGCATTTTTGCCAGCGTGGATTTACCCGAACCATTCTCGCCAATAATCGCCa         <  1:200602/148‑1 (MQ=255)
                                                                                                                                      aTGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCATTCCCGCCAGCATTTTTGCCAGCGTGGATTTACCCGAACCATTCTCGCCAATAATCGCCAg        <  8:427899/149‑1 (MQ=255)
                                                                                                                                            aTACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCATTCCCGCCAGCATTTTTGCCAGCGTGGATTTACCCGAACCATTCTCGCCAATAATCGCCAGTGGCTg  <  6:128211/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
CCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGGCTCTATCATTCCCGCCAGCATTTTTGCCAGCGTGGATTTACCCGAACCATTCTCGCCAATAATCGCCAGTGTCTG  >  NC_000913/1352226‑1352514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: