Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,693,416	A→G	5.2%	L283P (CTG→CCG)	uidB ←	glucuronide transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,693,416	0	A	G	5.2%	240.7 / 2.7	76	L283P (CTG→CCG)	uidB	glucuronide transporter
Reads supporting (aligned to +/- strand):  ref base A (31/41);  new base G (2/2);  total (33/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

CCAATTGAAGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGCACACACAGCGCACCGATGC  >  NC_000913/1693269‑1693563                                                                                                                                                    |                                                                                                                                                    ccAATTGAAGCGCTGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCa                                                                                                                                                     <  5:326565/148‑1 (MQ=255) ccAATTGAAGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGATCATCCCCGGCACCAg                                                                                                                                                    <  6:311627/149‑1 (MQ=255)         aGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCATAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGcc                                                                                                                                             <  4:154633/148‑1 (MQ=255)               ggCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCca                                                                                                                                      >  5:188582/1‑149 (MQ=255)                gCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCca                                                                                                                                      <  2:270825/148‑1 (MQ=255)                   aaCGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGt                                                                                                                                  <  8:95696/149‑1 (MQ=255)                        aaccaacGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTaccaa                                                                                                                             >  8:282493/1‑149 (MQ=255)                              cGCCACCGGCAGTGACCAGACGGTAACCCCGAAGTACAGCTGATTTCGTGAGGTTCCGAGCAAAGCTCCAAAAAGTTAGTTATTCTTTTTACCGATCCTCTAGTCCATCCCCCGCACCAGCTGTGCCGATGGCAAACTACCAACCAg                                                                                                                         <  4:21914/147‑1 (MQ=255)                                ccACCGGCAGTGACCTGATGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCGAGCAAAGCGCCATTCAGGTAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGttt                                                                                                                     <  1:366325/149‑1 (MQ=255)                                ccACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGttt                                                                                                                     >  5:88328/1‑149 (MQ=255)                                 cACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGtttt                                                                                                                    >  8:408907/1‑149 (MQ=255)                                  aCCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTg                                                                                                                   >  2:373171/1‑149 (MQ=255)                                   ccGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGc                                                                                                                  <  7:408907/149‑1 (MQ=255)                                        aGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAg                                                                                                             >  3:4599/1‑149 (MQ=255)                                         gTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCa                                                                                                                                                     <  5:354308/108‑1 (MQ=255)                                         gTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAACCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCa                                                                                                                                                     >  6:354308/1‑108 (MQ=255)                                           gACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTAc                                                                                                          >  6:299877/1‑149 (MQ=255)                                               aGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCAc                                                                                                                <  7:2267/139‑1 (MQ=255)                                               aGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCAc                                                                                                                >  8:2267/1‑139 (MQ=255)                                                   ggAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAg                                                                                                  >  3:66710/1‑149 (MQ=255)                                                    gAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGt                                                                                                 <  6:88328/149‑1 (MQ=255)                                                      aaCCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTg                                                                                                >  6:100013/1‑148 (MQ=255)                                                      aaCCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGa                                                                                               <  6:276935/149‑1 (MQ=255)                                                          cAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAg                                                                                           >  6:99814/1‑149 (MQ=255)                                                            gaagaaCAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGcc                                                                                         <  6:402946/149‑1 (MQ=255)                                                            gaagaaCAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGcc                                                                                         <  8:386341/149‑1 (MQ=255)                                                              agaaCAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCg                                                                                       <  5:41624/149‑1 (MQ=255)                                                                aaCAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCgg                                                                                      >  7:345957/1‑148 (MQ=255)                                                                 aCAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTa                                                                                    <  4:4599/149‑1 (MQ=255)                                                                   agcagATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAAc                                                                                             >  1:174999/1‑138 (MQ=255)                                                                   agcagATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAAc                                                                                             <  2:174999/138‑1 (MQ=255)                                                                      agATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCAtt                                                                               <  7:282493/149‑1 (MQ=255)                                                                       gATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGCTCCTCGCGACCCTCCCCCGCACCAGCGGGGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCCCAGTCAACCGCCCGGTATGAtt                                                                               >  8:401878/1‑148 (MQ=255)                                                                           aCCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAaca                                                                          <  5:144061/149‑1 (MQ=255)                                                                           aCCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAac                                                                           <  5:99814/148‑1 (MQ=255)                                                                           aCCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAac                                                                           <  5:100013/148‑1 (MQ=255)                                                                                       cAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCAc                                                                                                                >  1:7087/1‑99 (MQ=255)                                                                                       cAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCAc                                                                                                                <  2:7087/99‑1 (MQ=255)                                                                                           aaaGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGaa                                                          <  7:312394/149‑1 (MQ=255)                                                                                                 ccAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAacga                                                    <  8:105421/149‑1 (MQ=255)                                                                                                     tCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAg                                                <  3:324309/149‑1 (MQ=255)                                                                                                       aGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATATCGCACGTAGAACAACGACGCGgc                                              >  5:308270/1‑149 (MQ=255)                                                                                                         gAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAaca                                                                          >  8:275717/1‑119 (MQ=255)                                                                                                         gAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAaca                                                                          <  7:275717/119‑1 (MQ=255)                                                                                                         gAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGgcgc                                            <  5:299877/149‑1 (MQ=255)                                                                                                            ggTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAg                                                <  4:310466/142‑1 (MQ=255)                                                                                                            ggTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAg                                                >  3:310466/1‑142 (MQ=255)                                                                                                               aTTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAaca                                                                          >  4:34405/1‑113 (MQ=255)                                                                                                               aTTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAaca                                                                          <  3:34405/113‑1 (MQ=255)                                                                                                                ttCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGTGCTGACCg                                      >  2:16532/1‑148 (MQ=255)                                                                                                                 tCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAgc                                                                    <  6:259135/117‑1 (MQ=255)                                                                                                                 tCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAgc                                                                    >  5:259135/1‑117 (MQ=255)                                                                                                                   tttttACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGAc                                        <  5:385449/143‑1 (MQ=255)                                                                                                                    ttttACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAg                                 >  3:78342/1‑149 (MQ=255)                                                                                                                    ttttACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAg                                 >  3:83672/1‑149 (MQ=255)                                                                                                                     tttACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCaaa                                  <  4:66710/147‑1 (MQ=255)                                                                                                                         ccGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGa                                         <  1:88882/136‑1 (MQ=255)                                                                                                                         ccGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGa                                         >  2:88882/1‑136 (MQ=255)                                                                                                                          cGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGaa                           <  4:78342/149‑1 (MQ=255)                                                                                                                          cGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGaa                           <  4:83672/149‑1 (MQ=255)                                                                                                                            aTCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAAt                         >  4:196531/1‑149 (MQ=255)                                                                                                                            aTCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAAt                         >  7:164032/1‑149 (MQ=255)                                                                                                                             tCCTCGCGACCATCCCCGGCCCCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAAt                         >  6:214893/1‑148 (MQ=255)                                                                                                                               cTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCATCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAg                      <  6:188582/149‑1 (MQ=255)                                                                                                                               cTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAg                      <  1:388095/149‑1 (MQ=255)                                                                                                                                  gcgACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGca                    <  1:373171/148‑1 (MQ=255)                                                                                                                                  gcgACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGc                     <  3:196531/147‑1 (MQ=255)                                                                                                                                   cgACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGcaca                  <  8:345957/149‑1 (MQ=255)                                                                                                                                      ccATCCCCGGCACCGGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAgg                                <  3:213042/132‑1 (MQ=255)                                                                                                                                      ccATCCCCGGCACCGGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAgg                                >  4:213042/1‑132 (MQ=255)                                                                                                                                      ccATCCCCGGCACCGGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAgg                                >  4:218336/1‑132 (MQ=255)                                                                                                                                      ccATCCCCGGCACCGGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAgg                                <  3:218336/132‑1 (MQ=255)                                                                                                                                                cACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGaacaac                                                      >  5:234899/1‑100 (MQ=255)                                                                                                                                                cACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGaacaac                                                      <  6:234899/100‑1 (MQ=255)                                                                                                                                                cACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGTGCTGACCGCAAAGGTCGAAATCAGCACACACAGCGCACCGa     <  1:16532/149‑1 (MQ=255)                                                                                                                                                 aCCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGCACACACAGCGCACCGAt    >  5:42939/1‑149 (MQ=255)                                                                                                                                                   cAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGCACACACAGCGCACCGATg   >  2:222149/1‑148 (MQ=255)                                                                                                                                                   cAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGCACACACAGCGCACCGATg   >  6:352450/1‑148 (MQ=255)                                                                                                                                                   cAGAGGGGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTCCCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGGCGAAATCAGCACACACAGCGCACCGATGc  <  5:221741/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CCAATTGAAGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGCACACACAGCGCACCGATGC  >  NC_000913/1693269‑1693563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑