Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,753,390 C→A 5.2% T147T (ACG→ACT ydhV ← putative oxidoreductase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,753,3900CA 5.2% 292.0 / 2.6 90T147T (ACG→ACTydhVputative oxidoreductase subunit
Reads supporting (aligned to +/- strand):  ref base C (51/34);  new base A (2/3);  total (53/37)
Fisher's exact test for biased strand distribution p-value = 3.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GTTTTTCGAACCCATTATTGCGCCAGTTCCCGCACCGCCGCTGTGGTTACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTGCGCTGCAAAAA  >  NC_000913/1753243‑1753538
                                                                                                                                                   |                                                                                                                                                    
gTTTTTCGAACCCATTATTGCGCCAGTTCCCGCACCGCCGCTGTGGTTACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCcg                                                                                                                                                     >  2:108690/1‑149 (MQ=255)
     tCGAACCCATTATTGCGCCAGTTCCCGCACCGCCGCTGTGGTTACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAAAAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCcgtcgt                                                                                                                                                 <  6:318073/148‑1 (MQ=255)
     tCGAACCCATTATTGCGCCAGTTCCCGCACCGCCGCGGTGGTTACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCcgtcgt                                                                                                                                                 <  6:89948/148‑1 (MQ=255)
       gAACCCATTATTGCGCCAGTTCCCGCACCGCCGCTGTGGTTACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTgc                                                                                                                                              >  2:103099/1‑149 (MQ=255)
        aaCCCATTATTGCGCCAGTTCCCGCACCGCCGCTGTGGTTACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTcc                                                                                                                                                      <  5:15003/140‑1 (MQ=255)
        aaCCCATTATTGCGCCAGTTCCCGCACCGCCGCTGTGGTTACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTcc                                                                                                                                                      >  6:15003/1‑140 (MQ=255)
        aaCCCATTATTGCGCCAGTTCCCGCACCGCCGCTGTGGTTACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTgcg                                                                                                                                             >  2:274003/1‑149 (MQ=255)
        aaCCCATTATTGCGCCAGTTCCCGCACCGCCGCTGTGGTTACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTgcg                                                                                                                                             >  7:280415/1‑149 (MQ=255)
        aaCCCATTATTGCGCCAGTTCCCGCACCGCCGCTGTGGTTACGGCGATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCAAGCCTGACCAATAGCCGCCACACAGGGTTACGGACTGGTCAATCGACAAATTTCTTCCGTCGTTgcg                                                                                                                                             >  5:34234/1‑149 (MQ=255)
           ccATTATTGCGCCAGTTCCCGCACCGCCGCTGTTGTTACGGCTATTCAAGATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCTGACTGGTCAATCGACAAATTTCTTCCGTCTTTgc                                                                                                                                              <  7:285560/145‑1 (MQ=255)
           ccATTATTGCGCAAGTTCCCGCACCGCCGCTGTGGTTACGGCTATTCAACATGCCAGAGACAGGAACAAGGTTATCCCCAGCATGACCAATAGCCGCCACACAGGTATCCGGACTGCTCAATCGACAAATTTCTTCCGTCGTTgc                                                                                                                                              >  8:285560/1‑145 (MQ=255)
             attattGCGCCAGTTCCCGCACCGCCGCTGTGGTTACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGt                                                                                                                                        <  1:6084/149‑1 (MQ=255)
              ttattGCGCCAGTTCCATCACCGCCGCTGTGGTTACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGGCGCCACACAGGTGTCCGGACTGGTCAATCGACAAATTTCTTCCGTCTTTTCGCGCGTc                                                                                                                                       >  1:13356/1‑149 (MQ=255)
                       cAGTTCCCGCACCGCCGCTGTGGTTACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGCTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTccc                                                                                                                              <  4:95231/149‑1 (MQ=255)
                        aGTTCCCGCACCTCAGCTGTGGTTACGTCTATTCAACATGCCAGAGAGAGGACCAAGGGTTTCCCCAGCCTGACCAATCGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCTTTGCGCGCGTCCCTTTTcccc                                                                                                                             <  6:34234/149‑1 (MQ=255)
                         gTTCCCGCACCGCCGCTGTGGTTACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCCGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCa                                                                                                                            >  4:45989/1‑149 (MQ=255)
                         gTTCCCGCACCGCCGCTGTGGTTACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTcccc                                                                                                                             >  2:18996/1‑148 (MQ=255)
                              cGCACCGCCGCTGTGGTTACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAg                                                                                                                        >  6:262667/1‑148 (MQ=255)
                                  ccgccgCTGTGGTTACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATc                                                                                                                   >  6:4061/1‑149 (MQ=255)
                                   cgccgcTGTGGTTACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGaa                                                                                                                      >  3:30505/1‑145 (MQ=255)
                                   cgccgcTGTGGTTACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGaa                                                                                                                      <  4:30505/145‑1 (MQ=255)
                                   cgccgcTGTGGTTACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCAACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCg                                                                                                                  >  5:275452/1‑149 (MQ=255)
                                      cgcTGTGGTTACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCt                                                                                                               <  1:18996/149‑1 (MQ=255)
                                       gcTGTGGTTACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCtt                                                                                                              <  1:90390/149‑1 (MQ=255)
                                           tgGTTACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTc                                                                                                          >  6:395407/1‑149 (MQ=255)
                                                 cGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGc                                                                                                     <  5:262667/148‑1 (MQ=255)
                                                      aTTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACtt                                                                                               >  8:35492/1‑149 (MQ=255)
                                                       ttCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACCCAGGTTTACGGACTGGTCAATCGACAAATTTCTTCAGTCGTTGCTCGCGTCCCTTTTCCCCATAAGAAATCGGGTTTTTCCAGGCTAAAttt                                                                                              >  4:32412/1‑149 (MQ=255)
                                                       ttCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACttt                                                                                              >  7:153340/1‑149 (MQ=255)
                                                        tCAACATGCCAGAGATAGTCACAATGTTTTACCCAGCATTACAAATAGCCGCAAAACAGGTTTCAGGACTGTTAAATCGACAAATTTCTTCCTTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGTCTAACttt                                                                                              <  2:13356/148‑1 (MQ=255)
                                                          aaCATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGCCTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGt                                                                                            >  8:422300/1‑148 (MQ=255)
                                                             aTGGCAGAGAGAGGAACAAGGTTTTGCCCAGGCTGACCAATAGCCGCCACACAGGTTTCCGGACTTGTCAATCGACAAATTTCTTCCGTCGTTGCTCGCTTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACttt                                                                                              <  4:380657/143‑1 (MQ=255)
                                                             aTGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTATCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGCCTAACttt                                                                                              >  3:380657/1‑143 (MQ=255)
                                                              ttccAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTACTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAACAACTCGGCTTTTTCCAGGCTAACTTTGTCATCt                                                                                       >  3:49910/3‑149 (MQ=255)
                                                              tGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGTTCAATCGACAAAGTTCTTCCTTCGGTGCTCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAAATTTGTCATCt                                                                                       >  3:55153/1‑149 (MQ=255)
                                                              tGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCt                                                                                       >  5:212496/1‑149 (MQ=255)
                                                                   gagagagGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAAt                                                                                  >  7:75294/1‑149 (MQ=255)
                                                                   gagagagGAACAAGGTTTTCCCCAGACTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCAATAAGAAATCGGCTGTTTCCAGGCGAACTTTGTCATATGTAAt                                                                                  >  8:89325/1‑149 (MQ=255)
                                                                      agagGATCAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCtt                                                                               >  5:77840/1‑149 (MQ=255)
                                                                       gagGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGGCATCTTTAATCTTc                                                                              <  6:294699/149‑1 (MQ=255)
                                                                          gAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGc                                                                           <  5:395407/149‑1 (MQ=255)
                                                                          gAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGc                                                                           <  8:280415/149‑1 (MQ=255)
                                                                           aaCAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGc                                                                           >  7:323013/1‑148 (MQ=255)
                                                                             cAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCa                                                                         >  5:349911/1‑148 (MQ=255)
                                                                                ggTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATAcc                                                                     >  3:64204/1‑149 (MQ=255)
                                                                                  ttttCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCgg                                                                   <  1:274003/149‑1 (MQ=255)
                                                                                         cAGCCTGACAAATAGCCGAAACACAGGTTTCAGGACTTGTAAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGTTGATTTc                                                            <  8:192110/149‑1 (MQ=255)
                                                                                           gCCTGACCAATAGCCGCCACACAGGTTTCCGGTCTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGc                                                          <  8:98511/149‑1 (MQ=255)
                                                                                            ccTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTTATCTTTAATCTTCAGCCATACCGGTGATTTCGcc                                                         <  3:32412/149‑1 (MQ=255)
                                                                                               gACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGccttc                                                      >  1:95347/1‑149 (MQ=255)
                                                                                                aCCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGccttcc                                                     <  5:410470/149‑1 (MQ=255)
                                                                                                aCCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGccttc                                                      <  8:323013/148‑1 (MQ=255)
                                                                                                aCCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCAGTCGTTCCGCGCGTCCCTTTTCCCAATCAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATCCCGGTGATTTCGccttcc                                                     <  3:209173/149‑1 (MQ=255)
                                                                                                       gccgccACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGa                                               >  7:105413/1‑148 (MQ=255)
                                                                                                        ccgccACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGat                                              >  3:129838/1‑148 (MQ=255)
                                                                                                          gccACACAGGTTTCCGGACTGGTCAATCGACAATTTTCTTCAGTCGTTGCGCGCGTCCCTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATAc                                                                      <  5:336853/121‑1 (MQ=255)
                                                                                                          gccACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGataa                                            >  1:246710/1‑148 (MQ=255)
                                                                                                            gacacaGGTGTCCGGACTTGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGAtaataa                                         <  4:49910/148‑1 (MQ=255)
                                                                                                            cacacaGGTTTCCGGACTGGTCAATCGACACATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGAtaataa                                         <  4:55153/149‑1 (MQ=255)
                                                                                                            cacacaGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGAtaataa                                         <  7:35492/149‑1 (MQ=255)
                                                                                                            cacacaGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGAtaataa                                         <  6:77840/149‑1 (MQ=255)
                                                                                                            cacacaGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGAGTTCGCCTTCCCTTCGAtaataa                                         <  5:4061/149‑1 (MQ=255)
                                                                                                             acacaGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATaataat                                        >  5:139201/1‑149 (MQ=255)
                                                                                                             acacaGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATaataat                                        <  6:139201/149‑1 (MQ=255)
                                                                                                               acaGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGataa                                            >  5:87895/1‑143 (MQ=255)
                                                                                                               acaGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGataa                                            <  6:87895/143‑1 (MQ=255)
                                                                                                               acaGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGa                                      >  5:249724/1‑149 (MQ=255)
                                                                                                               acaGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGa                                      >  4:185935/1‑149 (MQ=255)
                                                                                                               acaGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTAGCGCGCGTCCCTTTTCCCCATAAAAAAGCGGCTTTTTCCAGGCTACGTTTGGCATGTTTAATGTTCAGCAATACCGGTGATTTCGCCTGCCCTACGATAATAATGa                                      >  8:354484/1‑149 (MQ=255)
                                                                                                                caGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGAc                                     <  1:108690/149‑1 (MQ=255)
                                                                                                                caGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTACAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGAc                                     <  1:103099/149‑1 (MQ=255)
                                                                                                                   gTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGAcgtc                                  <  4:64204/149‑1 (MQ=255)
                                                                                                                       ccGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTAt                              >  7:265287/1‑149 (MQ=255)
                                                                                                                             tGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTc                                                                              >  2:195354/1‑95 (MQ=255)
                                                                                                                             tGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTc                                                                              <  1:195354/95‑1 (MQ=255)
                                                                                                                             tGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGc                         >  3:86040/1‑148 (MQ=255)
                                                                                                                             tGGTCAATCGACAAATTTCTTCAGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGataat                                           >  8:57687/1‑130 (MQ=255)
                                                                                                                             tGGTCAATCGACAAATTTCTTCAGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGataat                                           <  7:57687/130‑1 (MQ=255)
                                                                                                                              ggTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCg                        >  5:144013/1‑148 (MQ=255)
                                                                                                                               gTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGaa                      >  6:116962/1‑149 (MQ=255)
                                                                                                                                  aaTCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAAtt                    >  6:350676/1‑148 (MQ=255)
                                                                                                                                    tCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCa                 >  6:100040/1‑149 (MQ=255)
                                                                                                                                    tCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCa                 >  7:188268/1‑149 (MQ=255)
                                                                                                                                      gACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCAtt               >  3:295737/1‑149 (MQ=255)
                                                                                                                                      gACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCAtt               <  4:295737/149‑1 (MQ=255)
                                                                                                                                       aCAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCAttt              >  2:43285/1‑149 (MQ=255)
                                                                                                                                       aCAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCAttt              >  2:49547/1‑149 (MQ=255)
                                                                                                                                       aCAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTAAGCCATAACGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCAttt              <  7:89325/149‑1 (MQ=255)
                                                                                                                                        cAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTg             >  1:133648/1‑149 (MQ=255)
                                                                                                                                         aaaTTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTgc            >  3:281380/1‑149 (MQ=255)
                                                                                                                                         aaaTTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTgc            >  4:350661/1‑149 (MQ=255)
                                                                                                                                           aTTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTgcgc          >  1:9894/1‑149 (MQ=255)
                                                                                                                                           aTTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTgcgc          <  1:49547/149‑1 (MQ=255)
                                                                                                                                           aTTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTgcgc          <  1:43285/149‑1 (MQ=255)
                                                                                                                                           aTTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTgcgc          >  2:201738/1‑149 (MQ=255)
                                                                                                                                                  ccGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTGCGCTGCaaa    >  8:380898/1‑148 (MQ=255)
                                                                                                                                                   cgtcgtTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTACTCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTGCGCTGCaaaaa  <  7:422300/149‑1 (MQ=255)
                                                                                                                                                   cgtcgtTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTGCGCTGCaaaaa  <  3:45989/149‑1 (MQ=255)
                                                                                                                                                   cgtcgtTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTGCGCTGCaaaaa  <  6:249724/149‑1 (MQ=255)
                                                                                                                                                   cgtcgtTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTGCGATAATAATGACGTCGTATCCAGCGAATTTCATTTGCGCTGCaaaa   >  5:122525/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GTTTTTCGAACCCATTATTGCGCCAGTTCCCGCACCGCCGCTGTGGTTACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTGCGCTGCAAAAA  >  NC_000913/1753243‑1753538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: