Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,963,803 G→A 6.9% A416A (GCC→GCT)  flhA ← putative flagellar export pore protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,963,8030GA 6.9% 175.2 / 3.1 60A416A (GCC→GCT) flhAputative flagellar export pore protein
Reads supporting (aligned to +/- strand):  ref base G (30/24);  new base A (2/2);  total (33/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.71e-01

TCCAGATAGCATTCAGGCCAAATGCCGGATCGACGGTCGCCTCACCAGGTAACGTCCCGGCAGCGGTTCCAGGGTTAATCGCCAGCCAGCGCCCCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTCTTGCGGATACTGCGTATACGGCCCAACAACTCACCATCCTGCTGGAAATCGACCATCGGGATCAGTCGATAA  >  NC_000913/1963655‑1963947
                                                                                                                                                    |                                                                                                                                                
tCCAGATAGCATTCAGGCCAAATGCCGGATCGACGGTCGCCTCACCAGGTAACGTCCCGGCAGCGGTTCCAGGGTTAATCGCCAGCCAGCGCCCCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCgg                                                                                                                                                  >  3:81629/1‑149 (MQ=255)
           ttCAGGCCAAATGCCGGATCGACGGTCGCCTCACCAGGTAACGTCCCGGCAGCGGTTCCAGGGTTAATCGCCAGCCAGCGCCCCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTg                                                                                                                                          >  4:160593/1‑146 (MQ=255)
             cAGGCCAAAAGCCGGTTCGTCGGTCGCCTCAGCAGCTAACGTCCCGGCTGCGGTTCCAGGGTTAATCGCCAGCCAGCGCCGCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAgt                                                                                                                                      <  1:387049/148‑2 (MQ=255)
                     aTGCCGGATCGACGGTCGCCTCACCAGGTAACGTCCCGGCAGCGGTTCCAGGGTTAATCGCCAGCCAGCGCCCCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTg                                                                                                                             <  7:303874/149‑1 (MQ=255)
                         cGGATCGACGGTCGCCTCACCAGGTAACGTCCCGGCAGCGGTTCCAGGGTTAATCGCCAGCCAGCGCCCCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTcgc                                                                                                                         <  1:209061/149‑1 (MQ=255)
                         cGGATCGACGGTCGCCTCACCAGGTAACGTCCCGGCAGCGGTTCCAGGGTTAATCGCCAGCCAGCGCCCCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTcgc                                                                                                                         <  1:423908/149‑1 (MQ=255)
                           gATCGACGGTCGCCTCACCAGGTAACGTCCCGGCAGCGGTTCCAGGGTTAATCGCCAGCCAGCGCCCCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTcgcg                                                                                                                        >  8:49221/1‑148 (MQ=255)
                                   gTCGCCTCACCAGGTAACGTCCCGGCAGCGGTTCCAGGGTTAATCGCCAGCCAGCGCCCCTGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGcac                                                                                                               <  8:418210/149‑1 (MQ=255)
                                       ccTCACCAGGTAACGTCCCGGCAGCGGTTCCAGGGTTAATCGCCAGCCAGCGCCCCGGATAAGCATCACCACGGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGcacca                                                                                                             >  2:266295/1‑147 (MQ=255)
                                              aGGTAACGTCCCGGCAGCGGTTCCAGGGTTAATCGCCAGCCAGCGCCCCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCa                                                                                                    <  4:81629/149‑1 (MQ=255)
                                                gTAACGTCCCGGCAGCGGTTCCAGGGTTAATCGCCAGCCAGCGCCCCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAg                                                                                                   >  7:234382/1‑148 (MQ=255)
                                                gTAACGTCCCGGCAGCGGTTCCAGGGTTAATCGCCAGCCAGCGCCCCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAg                                                                                                   >  7:146625/1‑148 (MQ=255)
                                                gTAACGTCCCGGCAGCGGTTCCAGGGTTAATCGCCAGCCAGCGCCCCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAg                                                                                                   <  8:234382/148‑1 (MQ=255)
                                                gTAACGTCCCGGCAGCGGTTCCAGGGTTAATCGCCAGCCAGCGCCCCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAg                                                                                                   >  1:192210/1‑148 (MQ=255)
                                                     gTCCCGGCAGCGGTTCCAGGGTTAATCGCCAGCCAGCGCCCCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCCGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATcc                                                                                             >  8:118030/1‑149 (MQ=255)
                                                     gTCCCGGCAGCGGTTCCAGGGTTAATCGCCAGCCAGCGCCCCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATcc                                                                                             >  2:275699/1‑149 (MQ=255)
                                                     gTCCCGGCAGCGGTTCCAGGGTTAATCGCCAGCCAGCGCCCCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATcc                                                                                             >  7:230792/1‑149 (MQ=255)
                                                      tCCCGGCAGCGGTTCCAGGGTTAATCGCCAGCCAGCGCCCCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAAtg                                                                                                                    <  8:88251/125‑1 (MQ=255)
                                                      tCCCGGCAGCGGTTCCAGGGTTAATCGCCAGCCAGCGCCCCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAAtg                                                                                                                    >  7:88251/1‑125 (MQ=255)
                                                      tCCCGGCAGCGGTTCCAGGGTTAATCGCCAGCCAGCGCCCCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATcc                                                                                             >  8:75567/1‑148 (MQ=255)
                                                       cccGGCAGCGGTTCCAGGGTTAATCGCCAGCCAGCGCCCCTGATAAGAATCAACACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCTGGAAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCTGCAGCAATCCCa                                                                                           >  1:251819/1‑149 (MQ=255)
                                                           gCAGCGGTTCCAGGGTTAATCGCCAGCCAGCGCCCCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCAtctc                                                                                       <  8:146625/149‑1 (MQ=255)
                                                                      aGGGTTAATCGCCAGCCAGCGCCCCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAAtt                                                                            >  1:125842/1‑149 (MQ=255)
                                                                        ggTTAATCGCCAGCCAGCGCCCCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTc                                                                          >  8:201061/1‑149 (MQ=255)
                                                                        ggTTAATCGCCAGCCAGCGCCCCGGAAAAGCATCACCACTCCCAATCTCCACGCCTTACCTCAAAATGCGATAGCGGGAAGGTAGCAGAGCCATTTTGTCGCGAATGAGCACCCCTGGCGGCCCCAAACCCATCTCCTCGGCAAATGTc                                                                          >  6:78986/1‑149 (MQ=255)
                                                                          tttaTCGCCAGCCAGCGCCCCGGCTCAGCATCACCACAGCCAATCTCCCCGCCGTTCATCAAAATGCGCTAGCGGGCAGGTTGCAGATCCCTATTGTCGCGACTGTGCACCACGGGCGGCAGATGTCCGCTCTCCTGGGCAAATTTCtt                                                                        >  4:105337/4‑149 (MQ=255)
                                                                          ttAATCGCCAGCCAGCGCCCCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTCtt                                                                        <  7:201061/149‑1 (MQ=255)
                                                                          gtAATCGACAGACAGCGACCAGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTCtt                                                                        <  2:251819/148‑1 (MQ=255)
                                                                             aTCGCCAGCCAGCGCCCCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTCTTGc                                                                      <  3:160593/148‑1 (MQ=255)
                                                                              tCGCCAGCCAGCGCCCCGGATAAGCAT‑‑CCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACAACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTCTTGCGGa                                                                   >  2:56033/1‑148 (MQ=255)
                                                                               cGCCAGCCAGCGCCCCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTCTTGCGGa                                                                   >  5:200454/1‑149 (MQ=255)
                                                                                gccagccaGCGCCCCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCTGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTCTTGCGGa                                                                   <  7:118030/148‑1 (MQ=255)
                                                                                gccagccaGCGCCCCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTCTTGCGGa                                                                   <  6:346326/148‑1 (MQ=255)
                                                                                   agccagcGCCCCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTCTTGCGGATACt                                                               <  8:230792/149‑1 (MQ=255)
                                                                                     ccagcGCCCCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTCTTGc                                                                      >  6:80097/1‑140 (MQ=255)
                                                                                     ccagcGCCCCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTCTTGc                                                                      <  5:80097/140‑1 (MQ=255)
                                                                                      cagcGCCCCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTCTTGCGGATACTGCg                                                            >  2:274350/1‑149 (MQ=255)
                                                                                                 aTAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGCGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTCTTGCGGATACTGCGTATACGGccc                                                  >  5:402334/1‑148 (MQ=255)
                                                                                                  tAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCTGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTCTTGCGGATACTGCGTATACGGCCcaa                                                >  6:402133/1‑149 (MQ=255)
                                                                                                  tAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTCTTGCGGATACTGCGTATACGGCCcaa                                                >  6:357980/1‑149 (MQ=255)
                                                                                                     gCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTCTTGCGGATACTGCGTATACGGCCcaacaa                                             >  5:1999/1‑149 (MQ=255)
                                                                                                      cATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATCGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTCTTGCGGATACTGCGTATACGGCCCaacaac                                            <  1:275699/149‑1 (MQ=255)
                                                                                                        tCACCATTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGAGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTCTTGCGGATACTGCGTATACGGCCCaacaac                                            <  5:300623/147‑1 (MQ=255)
                                                                                                        tCACCATTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGAGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTCTTGCGGATACTGCGTATACGGCCCaacaac                                            <  5:295079/147‑1 (MQ=255)
                                                                                                        tCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCTATAGCGGGAAGGTTGCAGATCCATATTGTCGCGAATGTTCACCACTGGCGGCAGAAATCCCATCGCCTGGGAAAATTTCTTGCGGATACTGCGTATACGGCCCCACAACt                                           >  8:184656/1‑148 (MQ=255)
                                                                                                        tCACCAATGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGAGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTCTTGCGGATACTGCGTATACGGCCCaacaac                                            >  6:300623/1‑147 (MQ=255)
                                                                                                        tCACCAATGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGAGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTCTTGCGGATAATGCGTATACGGCCCaacaac                                            >  6:295079/1‑147 (MQ=255)
                                                                                                                 ccAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTCTTGCGGATACTGCGTATACGGCCCAACAACTCACCATCctg                                 >  2:33530/1‑149 (MQ=255)
                                                                                                                     tctcCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTCTTGCGGATACTGCGTATACGGACCAACAACTCACCATCctgctg                              >  7:406010/1‑148 (MQ=255)
                                                                                                                          aCGCCTTTGATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTACTTGAGGATACTGAGTATACGTCACAACAACTCACCATCCTGCTGGAAATc                        >  3:330716/1‑149 (MQ=255)
                                                                                                                          aCGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTCTTGCGGATACTGCGTATACGGCCCAACAACTCACCATCCTGCTGGAAATc                        <  6:1999/149‑1 (MQ=255)
                                                                                                                           cGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTCTTGCGGATACTGCGTATACGGCCCAACAACTCACCATCCTGCTGGAAATc                        >  7:121352/1‑148 (MQ=255)
                                                                                                                            gCCTTTCATCAGAATGCGATTGCGGGCAGGTTGCTGATCCATTTTGTCGCGAATGTGCAGCACTGGCGGCAGAAATCCCCTCTCCTGGGCAAATTTGTTGCGGATACTGCGTATACGGCCCAACAACTCACCATCCTGCTGGAATTCGa                      <  5:78986/149‑1 (MQ=255)
                                                                                                                             ccTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTCTTGCGGATACTGCGTATACGGCCCAACAACTCACCATCCTGCTGGAAATCGAc                     >  5:104048/1‑149 (MQ=255)
                                                                                                                                   atcaAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTCTTGCGGATACTGCGTATACGGCCCAACAACTCACCATCCTGCTGGAAATCGACCATCg                <  6:104048/148‑1 (MQ=255)
                                                                                                                                    tcaAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGcacca                                                                                                             <  6:108194/54‑1 (MQ=255)
                                                                                                                                    tcaAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGcacca                                                                                                             >  5:108194/1‑54 (MQ=255)
                                                                                                                                    tcaAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTCTTGCGGATACTGCGTATACGGCCCAACAACTCACCATCCTGCTGGAAATCGACCATCggg              <  7:49221/149‑1 (MQ=255)
                                                                                                                                      aaaaTGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTCTTGCGGATACTGCGTATACGGCCCAACAACTCACCATCCTGCTGGAAATCGACCATCGGGAt            <  7:75567/149‑1 (MQ=255)
                                                                                                                                             gATAGCGTGAAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGTGCAAATTTCTTGCGGATACTGCGTATACGGCCCAACAACTCACCATCCTGCTGGAAATCGACCATCGGGATCAGTCGa     <  7:184656/149‑1 (MQ=255)
                                                                                                                                                 gCGGGCCGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTCATGCGGATACTGCGTATACGGCCCAACAACTCACCATCCTGCTGGAAATCGACCATCGGGATCAGTCGATaa  <  2:192210/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
TCCAGATAGCATTCAGGCCAAATGCCGGATCGACGGTCGCCTCACCAGGTAACGTCCCGGCAGCGGTTCCAGGGTTAATCGCCAGCCAGCGCCCCGGATAAGCATCACCACTGCCAATCTCCACGCCTTTCATCAAAATGCGATAGCGGGCAGGTTGCAGATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTCTTGCGGATACTGCGTATACGGCCCAACAACTCACCATCCTGCTGGAAATCGACCATCGGGATCAGTCGATAA  >  NC_000913/1963655‑1963947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑