Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,745,019 T→A 5.7% K107N (AAA→AAT) ‡ trmD ← tRNA m(1)G37 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,745,0190TA 5.7% 345.6 / 6.2 108K107N (AAA→AAT) ‡trmDtRNA m(1)G37 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (46/55);  new base A (4/3);  total (50/58)
Fisher's exact test for biased strand distribution p-value = 7.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

GAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCG  >  NC_000913/2744871‑2745167
                                                                                                                                                    |                                                                                                                                                    
gAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAAt                                                                                                                                                      >  1:362123/1‑149 (MQ=255)
gAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAAt                                                                                                                                                      <  1:42433/149‑1 (MQ=255)
     aaCCGGGAAACGGAGTCAATCAGCTTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCCTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAAttttt                                                                                                                                                  <  4:74386/148‑1 (MQ=255)
     aaCCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAAttttt                                                                                                                                                  <  4:342128/148‑1 (MQ=255)
      aCCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGa                                                                                                                                                <  7:356771/149‑1 (MQ=255)
           gAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAAAAATTGATTctg                                                                                                                                           <  1:134325/149‑3 (MQ=255)
                      aaTCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGCCCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGCCCGCCCACCAGAATCAATTTTTGATTCGTTGCCAGTTCGc                                                                                                                                >  3:9562/1‑149 (MQ=255)
                      aaTCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGtt                                                                                                                                          >  5:338191/1‑139 (MQ=255)
                      aaTCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGtt                                                                                                                                          <  6:338191/139‑1 (MQ=255)
                       aTCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCt                                                                                                                               >  4:366397/1‑149 (MQ=255)
                        tCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTg                                                                                                                              <  8:4520/149‑1 (MQ=255)
                          aGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGAc                                                                                                                            <  3:340116/149‑1 (MQ=255)
                               cATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCg                                                                                                                       >  2:87038/1‑149 (MQ=255)
                                aTTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGc                                                                                                                                        >  4:336863/1‑131 (MQ=255)
                                aTTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGc                                                                                                                      <  5:407099/149‑1 (MQ=255)
                                 atGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCAATCTTCGTCAATTACGGTTTGCATCACGCGCTCAACTATACCTTCGTACCGACCGCACACCAGAATCAAATTTTGAATCGTTGCCAGTTCGCTAAAGCCCGCt                                                                                                                     >  4:129144/2‑149 (MQ=255)
                                          aCTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGc                                                                                                            <  8:49226/149‑1 (MQ=255)
                                          aCTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGc                                                                                                            <  7:66250/149‑1 (MQ=255)
                                            tCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGa                                                                                                                                                <  4:214590/111‑1 (MQ=255)
                                            tCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGa                                                                                                                                                >  3:214590/1‑111 (MQ=255)
                                            tCACCACCACTGAGAACGTAATAGCCGATTGCCCATTCTTCGTCAATTTCGGATTGGATCACCCGCACATAAATACCTTCGTAGCGACCGCACACCAGAATCAAATTTAGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCtt                                                                                                          >  2:425516/1‑149 (MQ=255)
                                              accaccacTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTg                                                                                                         <  3:119897/148‑1 (MQ=255)
                                              accaccacTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTGGGATCACGCGCTCAGCTATACCTTCGTCGCGACCGCACACCAGAAGCACTTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCtt                                                                                                          <  6:373277/147‑1 (MQ=255)
                                                caccacTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGt                                                                                                      >  3:61704/1‑149 (MQ=255)
                                                  ccacTGAGAACGTAATCGCCGATTGCCCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTcc                                                                                                    >  5:290499/1‑149 (MQ=255)
                                                   cacTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGtt                                                                                                                                   >  8:1351/1‑117 (MQ=255)
                                                   cacTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGtt                                                                                                                                   <  7:1351/117‑1 (MQ=255)
                                                    acTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCt                                                                                                  <  2:286303/149‑1 (MQ=255)
                                                      tGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCCCGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTGGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATAAAGCTTGCGTCCCtgt                                                                                                >  8:295296/1‑149 (MQ=255)
                                                        agaACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCTCGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGTTTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCtgttg                                                                                              <  3:19681/149‑3 (MQ=255)
                                                        agaACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGc                                                                                                        <  5:36010/139‑1 (MQ=255)
                                                        agaACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCtgtg                                                                                               <  7:275247/148‑1 (MQ=255)
                                                        agaACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCtgtg                                                                                               <  2:395890/148‑1 (MQ=255)
                                                         gaACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggt                                                                                             <  7:104876/149‑1 (MQ=255)
                                                            cGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGAc                                                                                          <  6:290499/149‑1 (MQ=255)
                                                            cGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGAc                                                                                          <  3:331219/149‑1 (MQ=255)
                                                            cGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAAAAATTGATTctggt                                                                                                                                         >  3:108530/1‑98 (MQ=255)
                                                            cGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAAGCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGAc                                                                                          <  2:121413/149‑1 (MQ=255)
                                                              tAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAg                                                                                        <  4:61704/149‑1 (MQ=255)
                                                                aTCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGa                                                                                       <  1:7340/148‑1 (MQ=255)
                                                                aTCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGa                                                                                       <  4:248936/148‑1 (MQ=255)
                                                                 tCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggt                                                                                             >  3:304823/1‑141 (MQ=255)
                                                                 tCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggt                                                                                             <  4:304823/141‑1 (MQ=255)
                                                                   gCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCtgtg                                                                                               <  1:268376/137‑1 (MQ=255)
                                                                   gCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACAAGAATCAATTTTTGATTAGTTTCCAGTTCGCTGACGCCCGCTTGATCAAGATTGCGTCCCtctg                                                                                               >  2:268376/1‑137 (MQ=255)
                                                                      gATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGc                                                                                                        <  5:372392/125‑1 (MQ=255)
                                                                      gATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGc                                                                                                        >  6:372392/1‑125 (MQ=255)
                                                                      gATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCa                                                                                >  6:6034/1‑149 (MQ=255)
                                                                      gATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCa                                                                                <  2:362123/149‑1 (MQ=255)
                                                                           tccATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGGACACGAGAATCAATTTTTGATTCGTTGCCTGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCtt                                                                            <  3:129144/147‑1 (MQ=255)
                                                                              aTTCTTCGTCAATTTCGGTTTGGATCACTCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTTCCAGTTCGATGACGCCCGCTTGATCAAGCTTGCGTCCATGTGGTGACATATAAATCACCTTTgcg                                                                        >  8:106360/1‑149 (MQ=255)
                                                                               ttcttcGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTgcgc                                                                       >  6:277209/1‑149 (MQ=255)
                                                                                 cttcGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGc                                                                                                                                        <  7:334435/82‑1 (MQ=255)
                                                                                 cttcGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGc                                                                                                                                        >  8:334435/1‑82 (MQ=255)
                                                                                   tcGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGcc                                                                                                           <  8:404182/109‑2 (MQ=255)
                                                                                   tcGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGcc                                                                                                           >  7:404182/1‑108 (MQ=255)
                                                                                    cGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCt                                                                     <  3:366397/146‑1 (MQ=255)
                                                                                         aTTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACccgc                                                             <  7:260431/149‑1 (MQ=255)
                                                                                          tttCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCa                                                                  >  1:132389/1‑143 (MQ=255)
                                                                                          tttCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCa                                                                  <  2:132389/143‑1 (MQ=255)
                                                                                          tttCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACccgcc                                                            >  7:55018/1‑149 (MQ=255)
                                                                                                tttGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCt                                                      >  3:215533/1‑149 (MQ=255)
                                                                                                tttGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCt                                                      >  4:301627/1‑149 (MQ=255)
                                                                                                tttGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACACGCCGCGGCt                                                      >  3:111901/1‑149 (MQ=255)
                                                                                                   ggATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCttt                                                    <  5:6034/148‑1 (MQ=255)
                                                                                                         cgcgCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGAGCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCa                                             <  1:87038/149‑1 (MQ=255)
                                                                                                          gcgcTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                                                                                                             <  3:277794/84‑1 (MQ=255)
                                                                                                          gcgcTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                                                                                                             >  4:277794/1‑84 (MQ=255)
                                                                                                            gcTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGa                                                                                                                  <  8:344288/77‑1 (MQ=255)
                                                                                                             cTCATCTATACCTTCGTAGCGACCGCACACCAGAAGCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCGTGATCAAGCGTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGaa                                         <  7:295296/149‑1 (MQ=255)
                                                                                                              tCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATc                                                                                                                >  1:126736/1‑77 (MQ=255)
                                                                                                              tCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATc                                                                                                                <  2:126736/77‑1 (MQ=255)
                                                                                                                aTCTATACCTTCGTAGCGCCCGCACACCAGAATGAATTTTTGATTCGTTGCCAGGTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGGGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATg                                       <  1:425516/148‑1 (MQ=255)
                                                                                                                aTCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATgg                                      >  7:235245/1‑149 (MQ=255)
                                                                                                                aTCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATg                                       <  6:53214/148‑1 (MQ=255)
                                                                                                                aTCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATg                                       >  5:53214/1‑148 (MQ=255)
                                                                                                                 tCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGaa                                         >  6:258614/1‑145 (MQ=255)
                                                                                                                 tCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGaa                                         <  5:258614/145‑1 (MQ=255)
                                                                                                                  cTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCg                                    >  4:44667/1‑149 (MQ=255)
                                                                                                                    ataCCTTCGTAGCGACCGCACCCCAGAATCAATATTTGATTAGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCGTGCGTCCCTGTTGTGACAGAAAAATCACCTTTGCGCCTTCCCCCGCCGCGGCGTTTGCTGCCTGAATGGCGTc                                  >  6:296871/1‑149 (MQ=255)
                                                                                                                          tCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCa                                             >  4:74136/1‑132 (MQ=255)
                                                                                                                          tCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCa                                             <  3:74136/132‑1 (MQ=255)
                                                                                                                            gaaGCGACCTCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAg                           <  5:296871/146‑1 (MQ=255)
                                                                                                                                cGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTg                      <  3:301627/149‑1 (MQ=255)
                                                                                                                                  aCCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGCCGCCCGCGTGATCAAg                                                                                                             >  2:260254/1‑60 (MQ=255)
                                                                                                                                  aCCGCACACAAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGATTGATCAAg                                                                                                             <  1:260254/60‑1 (MQ=255)
                                                                                                                                       acacCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCCCcat                >  6:271794/1‑148 (MQ=255)
                                                                                                                                        cacCAGAAGCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACcatca              <  2:194335/149‑1 (MQ=255)
                                                                                                                                         acCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACcatcat             >  5:167368/1‑149 (MQ=255)
                                                                                                                                          ccAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACcatcat             >  7:188139/1‑148 (MQ=255)
                                                                                                                                          aacgAATCAAAAATTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTtg                                                  <  2:367761/108‑1 (MQ=255)
                                                                                                                                          aacgAATCAAAAATTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTtg                                                  >  1:367761/4‑111 (MQ=255)
                                                                                                                                           cAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCAtt            >  6:258102/1‑148 (MQ=255)
                                                                                                                                            aGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAAt                                        >  8:167573/1‑119 (MQ=255)
                                                                                                                                                tCAATTTTTGATTCGTTGCCAGTTCGCTGGCGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATc      >  5:288945/1‑149 (MQ=255)
                                                                                                                                                  aaTTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATccc    <  3:44667/149‑1 (MQ=255)
                                                                                                                                                  aaTTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATccc    >  5:174570/1‑149 (MQ=255)
                                                                                                                                                    tttttGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTTGTGACAGATAAATGAACTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGTCGTCCCTCAAGGGTTGCACCATCATTAACATCGCCg  <  7:328953/149‑1 (MQ=255)
                                                                                                                                                    tttttGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCg  <  4:215533/149‑1 (MQ=255)
                                                                                                                                                    tttttGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCg  >  5:355560/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCG  >  NC_000913/2744871‑2745167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: