Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,482,758 G→A 6.4% H360H (CAC→CAT)  valS ← valyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,482,7580GA 6.4% 298.7 / 6.1 94H360H (CAC→CAT) valSvalyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (41/46);  new base A (3/3);  total (45/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACACCTGG  >  NC_000913/4482613‑4482905
                                                                                                                                                 |                                                                                                                                                   
aCTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGc                                                                                                                                                  >  7:17381/1‑149 (MQ=255)
   gCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGt                                                                                                                                               >  6:48060/1‑149 (MQ=255)
    cTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGtt                                                                                                                                              <  4:176532/149‑1 (MQ=255)
    cTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGtt                                                                                                                                              <  4:170431/149‑1 (MQ=255)
          tACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAAttt                                                                                                                                        >  8:22215/1‑149 (MQ=255)
          tACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAAttt                                                                                                                                        >  2:343704/1‑149 (MQ=255)
              aaCTGACTGGCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACAGCGGCACGCCCGGACCACTGGTCGGTCAGCATTGGTTCGATAACTCCGCCGCCACGGTCGCCGTAAGGAACGGGCAGGTCGTGCGGTTTAATttcttc                                                                                                                                    <  8:306433/149‑1 (MQ=255)
                   aaTGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCcagca                                                                                                                               <  1:138540/149‑1 (MQ=255)
                   aaTGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCcag                                                                                                                                 <  1:228939/147‑1 (MQ=255)
                   aaTGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCcag                                                                                                                                 <  1:97017/147‑1 (MQ=255)
                      gTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCATGCGGTTTAATTTCTTCCAGCAGGc                                                                                                                            >  7:77859/1‑149 (MQ=255)
                      gTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCATGCGGTTTAATTTCTTCCAGCAGGc                                                                                                                            >  1:398653/1‑149 (MQ=255)
                      gTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGGCGCCGTAAGGAACGGTCAGGTCATGCGGTTTAATTTCTTCCAGCAGGc                                                                                                                            >  1:404475/1‑149 (MQ=255)
                       tCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGcc                                                                                                                           <  8:343137/149‑1 (MQ=255)
                          ccGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAg                                                                                                                        >  4:193054/1‑149 (MQ=255)
                            gTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAgcg                                                                                                                      >  8:124840/1‑149 (MQ=255)
                            gTTCTCAACCGCTTCAACAGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAgcg                                                                                                                      >  3:343643/1‑149 (MQ=255)
                             ttCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAgcgc                                                                                                                     >  7:333024/1‑149 (MQ=255)
                             ttCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAgcgc                                                                                                                     <  3:267542/149‑1 (MQ=255)
                              tctcAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGcgcg                                                                                                                    >  3:3443/1‑149 (MQ=255)
                                tcAACCGCTTCACCCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGCTCGCCGGAAGGAACGTTCAGGGCGGGCGGTTTATTTTCTTCCAGCAGGCCAAGCGCGt                                                                                                                   >  5:360679/1‑148 (MQ=255)
                                 cAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTc                                                                                                                  >  1:416365/1‑148 (MQ=255)
                                  aaCCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCCGGCCAAGCGCGTCaa                                                                                                                >  2:216842/1‑149 (MQ=255)
                                  aaCCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCaa                                                                                                                >  7:240652/1‑149 (MQ=255)
                                  aaCCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCaa                                                                                                                >  1:360048/1‑149 (MQ=255)
                                       cgtCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTTGTTCGATAACTCCGCCGCCTCGGTCGCCGTAAGGAACTGTCAGGTCGTGGGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCg                                                                                                           <  6:407434/147‑1 (MQ=255)
                                        ttCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCgg                                                                                                          >  2:248410/1‑149 (MQ=255)
                                          cAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCgg                                                                                                          <  8:240652/147‑1 (MQ=255)
                                           aaCCGCCGGTTTCGCCAGGACATCGGCACGCACCTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCaa                                                                                                       >  6:47018/1‑149 (MQ=255)
                                               gccgGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGAc                                                                                                   <  7:124840/149‑1 (MQ=255)
                                                ccgGTTTCGCCAGGGCATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGGCGCCGTAGGGAACGGTCAGGTCGGGCGGTGTAATTTCTTCCGGCCGGCCGCGCGCGTCCCCGGCGGCAACGCCt                                                                                                  >  4:381298/1‑149 (MQ=255)
                                                    tttCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCtt                                                                                              <  4:394954/149‑1 (MQ=255)
                                                        gCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGTAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCtt                                                                                              >  2:332910/1‑145 (MQ=255)
                                                        gCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCtt                                                                                              <  1:332910/145‑1 (MQ=255)
                                                        gCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTAc                                                                                           <  1:180891/148‑1 (MQ=255)
                                                         ccAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGt                                                                                         <  2:239694/149‑1 (MQ=255)
                                                          cAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTg                                                                                        >  1:15112/1‑149 (MQ=255)
                                                           aGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTg                                                                                        >  5:145590/1‑148 (MQ=255)
                                                             gACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGc                                                                                                <  3:105587/138‑1 (MQ=255)
                                                             gACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGc                                                                                                >  4:105587/1‑138 (MQ=255)
                                                                 tCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGCTAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCaa                                                                                  >  4:371910/1‑148 (MQ=255)
                                                                   ggCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGGCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCaaaa                                                                                <  5:48060/148‑1 (MQ=255)
                                                                      acgcacgTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCt                                                                            <  3:193054/149‑1 (MQ=255)
                                                                        gcacgTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTc                                                                           >  6:287939/1‑148 (MQ=255)
                                                                        gcacgTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTc                                                                           >  8:48859/1‑148 (MQ=255)
                                                                         cacgTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTACTTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCa                                                                         <  3:52183/149‑1 (MQ=255)
                                                                         cacgTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTc                                                                           >  4:266606/1‑147 (MQ=255)
                                                                            gTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCTGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGtt                                                                      >  8:226251/1‑149 (MQ=255)
                                                                            gTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGtt                                                                      >  8:423838/1‑149 (MQ=255)
                                                                            gTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGtt                                                                      >  7:376309/1‑149 (MQ=255)
                                                                             tACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGttt                                                                     <  7:48859/149‑1 (MQ=255)
                                                                             tACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGttt                                                                     <  7:423838/149‑1 (MQ=255)
                                                                               ccACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTc                                                                    <  5:287939/148‑1 (MQ=255)
                                                                               ccACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCt                                                                   <  1:343704/149‑1 (MQ=255)
                                                                               ccACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCt                                                                   <  4:3443/149‑1 (MQ=255)
                                                                                cACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTg                                                                  <  2:360048/149‑1 (MQ=255)
                                                                                 aCTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCCCGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAAGAGAGGCCACGCCTGCTTTACGTGCAGCAAATCGCTCCAGTTTCTg                                                                  >  1:196748/1‑148 (MQ=255)
                                                                                     gtcggtcAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGaa                                                               <  8:333024/147‑1 (MQ=255)
                                                                                     gtcgctcACCATTGCTTCGATAACTAACCCGCCACGGACGCCGTAAGCAAAAAACACGTCCTGCGGTTTAATTTCTTCCAGAAGGCAAAGTGCGTCAACTGCGGCAACGAATCCATTACGTGCAGCAAAACGCACCACTATCTGGAAct                                                             >  6:62792/1‑149 (MQ=255)
                                                                                         gtcAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGc                                                         <  7:335882/149‑1 (MQ=255)
                                                                                            aGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCATGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAgg                                                      <  8:77859/149‑1 (MQ=255)
                                                                                            aGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCATGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAgg                                                      <  2:398653/149‑1 (MQ=255)
                                                                                            aGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCATGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTGTCTGGAACTCTGCAgg                                                      <  2:404475/149‑1 (MQ=255)
                                                                                              cATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGa                                                    <  7:251286/149‑1 (MQ=255)
                                                                                                ttGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGAtt                                                  <  8:331911/149‑1 (MQ=255)
                                                                                                   gTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCaa                                                                                                                <  1:147113/84‑1 (MQ=255)
                                                                                                   gTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCaa                                                                                                                >  2:147113/1‑84 (MQ=255)
                                                                                                   gTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTCAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGa                                                                                                    >  1:418415/1‑96 (MQ=255)
                                                                                                   gTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTCAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGa                                                                                                    <  2:418415/96‑1 (MQ=255)
                                                                                                    ttCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGc                                              >  4:151579/1‑149 (MQ=255)
                                                                                                       gATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTgg                                           <  8:17381/149‑1 (MQ=255)
                                                                                                       gATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTg                                            <  5:205871/148‑1 (MQ=255)
                                                                                                             tACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATaaa                                     >  1:20988/1‑149 (MQ=255)
                                                                                                               cgccgcCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAAc                                    <  3:381298/148‑1 (MQ=255)
                                                                                                               cgccgcCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTGCTTCCAGCAGGCCAAGCGCGTCTACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAAGTCTGCAGGGATTTCGCTGGAATaaa                                     <  5:17979/147‑1 (MQ=255)
                                                                                                                gccgccACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGt                                  >  4:303464/1‑149 (MQ=255)
                                                                                                                gccgccACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGt                                  >  4:90127/1‑149 (MQ=255)
                                                                                                                gacgccACGGTCGCCGTAAGGTTCGGGAAGGTCGTGCGGTTTAATTTCTTCCAGCAGTCACTGCGCTTCAACTGCCGCAACGACTGCTTTACGTGCAGCAAAAGGCGCCAGTTTCTCGAACTCTGCAGGGATTTCGCTGTTATAATCGt                                  <  6:360679/147‑1 (MQ=255)
                                                                                                                       cGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTc                           <  3:266606/149‑1 (MQ=255)
                                                                                                                       cGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTAAAGCAGTCCAAGCGCGTAAAATTATGCAACGACTGCTTTAAGTGCAGCAAAACGCTCAAGTTTATGGAAAAATGCAGAGATTTCGCTGGAATAAAAGGCAGAtac                           >  6:280218/1‑147 (MQ=255)
                                                                                                                         gTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGt                         <  2:416365/149‑1 (MQ=255)
                                                                                                                         gTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGt                         <  2:20988/149‑1 (MQ=255)
                                                                                                                            gCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTAc                      <  7:22215/149‑1 (MQ=255)
                                                                                                                                  aGGTACGGGCATGTAGTGCGTTTTAATTTCTTACATCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTTCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTgg                <  5:280218/149‑1 (MQ=255)
                                                                                                                                    gAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGaa                                         <  6:391265/122‑1 (MQ=255)
                                                                                                                                    gAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGaa                                         >  5:391265/1‑122 (MQ=255)
                                                                                                                                     aaCGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCCACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTAt             >  8:223718/1‑149 (MQ=255)
                                                                                                                                       cGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATc            >  1:81454/1‑148 (MQ=255)
                                                                                                                                         gTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCTCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGaa         <  4:343643/149‑1 (MQ=255)
                                                                                                                                            aGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAaca       <  7:226251/148‑1 (MQ=255)
                                                                                                                                              gTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTg                                                                  >  5:387686/1‑87 (MQ=255)
                                                                                                                                              gTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTg                                                                  <  6:387686/87‑1 (MQ=255)
                                                                                                                                              gTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTAt             >  5:303007/1‑140 (MQ=255)
                                                                                                                                              gTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTAt             <  6:303007/140‑1 (MQ=255)
                                                                                                                                                 gTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACACCTgg  <  8:191251/148‑1 (MQ=255)
                                                                                                                                                 gTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACACCTgg  <  3:303464/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
ACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACACCTGG  >  NC_000913/4482613‑4482905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑