Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 755,400 G→A W75* (TGG→TAG)  sdhC → succinate dehydrogenase, membrane subunit, binds cytochrome b556

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913755,4000GA97.9% 316.1 / ‑2.4 93W75* (TGG→TAG) sdhCsuccinate dehydrogenase, membrane subunit, binds cytochrome b556
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (47/44);  total (48/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

TTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTGCTTTCACTTCTC  >  NC_000913/755259‑755545
                                                                                                                                             |                                                                                                                                                 
ttCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATc                                                                                                                                            <  5:257555/149‑1 (MQ=255)
  ctcCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCt                                                                                                                                          >  2:90479/1‑149 (MQ=255)
     cATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTAc                                                                                                                                       >  1:139312/1‑149 (MQ=255)
      aTCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTAcc                                                                                                                                      >  5:277379/1‑149 (MQ=255)
       tCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCg                                                                                                                                     >  7:77726/1‑149 (MQ=255)
       tCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCg                                                                                                                                     >  6:71903/1‑149 (MQ=255)
          cgTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGctc                                                                                                                                  >  7:358876/1‑149 (MQ=255)
          cgTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGctc                                                                                                                                  <  3:144339/149‑1 (MQ=255)
               ccGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGc                                                                                                                              <  1:90479/148‑1 (MQ=255)
                   tgtgATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATc                                                                                                                         <  2:329079/149‑1 (MQ=255)
                    gtgCTCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCACGCTTCCGGGATTATGGGCAGGTGCTTCGTCATATTTATCATGTAGGGCATCCTTGCCGCTCTGGCGTATCa                                                                                                                        >  4:311373/1‑149 (MQ=255)
                       aTCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCAcgt                                                                                                                     >  8:337055/1‑149 (MQ=255)
                       aTCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCAcgt                                                                                                                     >  1:22786/1‑149 (MQ=255)
                       aTCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCAcgt                                                                                                                     >  3:164232/1‑149 (MQ=255)
                        tCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCAcgtc                                                                                                                    <  2:139312/149‑1 (MQ=255)
                           ccTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCAcgtcgtc                                                                                                                 <  7:267513/149‑1 (MQ=255)
                           ccTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCAcgtcgtc                                                                                                                 <  5:221600/149‑1 (MQ=255)
                           ccTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCAcgtcgtc                                                                                                                 <  1:134928/149‑1 (MQ=255)
                            cTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCAcgtcgtcg                                                                                                                >  2:261852/1‑149 (MQ=255)
                            cTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCAcgtcgtcg                                                                                                                >  6:170048/1‑149 (MQ=255)
                            cTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCAcgtcgtcg                                                                                                                >  5:257373/1‑149 (MQ=255)
                             tttGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCAcgtcgtcg                                                                                                                >  1:284340/1‑148 (MQ=255)
                                gttgCAGTGGGCATCCTGCTGTGGCTTCTGGGTTCCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAg                                                                                                             <  2:406022/148‑1 (MQ=255)
                                gttgCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAg                                                                                                             <  8:77726/148‑1 (MQ=255)
                                     aGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTc                                                                                                       <  2:117073/149‑1 (MQ=255)
                                       tGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGc                                                                                                     >  6:298579/1‑149 (MQ=255)
                                         ggCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCca                                                                                                   >  3:20702/1‑149 (MQ=255)
                                            aTCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGTGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACat                                                                                                >  1:232712/1‑149 (MQ=255)
                                             tCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACatg                                                                                               <  4:20702/149‑1 (MQ=255)
                                                 gctgTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACatgatga                                                                                           >  1:354119/1‑149 (MQ=255)
                                                  ctgTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACatgatgat                                                                                          <  7:337055/149‑1 (MQ=255)
                                                   tgtgGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACatgatgatg                                                                                         >  7:240664/1‑149 (MQ=255)
                                                     tgGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAgg                                                                                                            >  2:236718/1‑128 (MQ=255)
                                                     tgGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAgg                                                                                                            <  1:236718/128‑1 (MQ=255)
                                                           cTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTg                                                                                  <  8:57419/148‑1 (MQ=255)
                                                              ggTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCt                                                                                                                                          >  5:291263/1‑89 (MQ=255)
                                                              ggTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCt                                                                                                                                          <  6:291263/89‑1 (MQ=255)
                                                                  ccAGCCTCTCTTCCACTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCt                                                                           <  8:276911/148‑1 (MQ=255)
                                                                   cAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATc                                                                            <  5:282239/146‑1 (MQ=255)
                                                                   cAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATc                                                                            >  6:282239/1‑146 (MQ=255)
                                                                   cAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTg                                                                          >  5:110000/1‑148 (MQ=255)
                                                                    aGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAg                                                                                                             >  1:141537/1‑112 (MQ=255)
                                                                    aGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAg                                                                                                             <  2:141537/112‑1 (MQ=255)
                                                                      ccTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGgaag                                                                      <  5:71903/149‑1 (MQ=255)
                                                                        acgctTCCCATGAAGGTTTCGAGCAAGCTTCCGCGATTCTGGGCAGCTTATTCGTCAACTTTATCAGGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGAGGGATTTGGGCTATCTGgaagaa                                                                    <  6:277379/146‑1 (MQ=255)
                                                                            ttCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGaaa                                                                   <  6:234421/146‑1 (MQ=255)
                                                                              ccccTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAATTTTATAATGTAGGGCATCCTTACCGATCTGTGGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACAt                                                                <  1:214426/147‑1 (MQ=255)
                                                                              ccccTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACAt                                                                <  8:358876/147‑1 (MQ=255)
                                                                              ccccTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACa                                                                 <  4:164232/146‑1 (MQ=255)
                                                                              ccccTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACAt                                                                <  2:284340/147‑1 (MQ=255)
                                                                               cccTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCATCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTc                                                              >  7:11979/1‑148 (MQ=255)
                                                                                ccTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGAtttt                                                                                   <  7:331704/126‑1 (MQ=255)
                                                                                ccTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGAtttt                                                                                   >  8:331704/1‑126 (MQ=255)
                                                                                ccTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCg                                                             >  7:362461/1‑148 (MQ=255)
                                                                                ccTGAAGGTTTCGAGCAAGCTTCCGCGAGTATGGGCCGCTTCGTCGTCAAATTTATCCTGTAGGGCCTCCTTACCGCTCTGGCGTATCCCGTCGTCGTAGGTATTCGCCACCTGAGGATGGATTGTGGCTATATGGAAGCAACATTCg                                                             >  1:321767/1‑148 (MQ=255)
                                                                                    aaGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGc                                                         <  5:170048/148‑1 (MQ=255)
                                                                                       gTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCggg                                                      >  6:149570/1‑148 (MQ=255)
                                                                                        tttCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCggg                                                      <  4:248464/147‑1 (MQ=255)
                                                                                        tttCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGt                                                     <  5:298579/148‑1 (MQ=255)
                                                                                        tttCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGt                                                     >  8:17989/1‑148 (MQ=255)
                                                                                        tttCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTa                                                    <  1:115489/149‑1 (MQ=255)
                                                                                             aGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGcc                                                                                                    <  6:145732/96‑1 (MQ=255)
                                                                                             aGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGcc                                                                                                    >  5:145732/1‑96 (MQ=255)
                                                                                              gCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCt                                              >  8:232286/1‑149 (MQ=255)
                                                                                                  gCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGc                                          <  6:110000/149‑1 (MQ=255)
                                                                                                    ttCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGcc                                         >  1:311705/1‑148 (MQ=255)
                                                                                                        gcgATTATTGGCAGCTTCTTCGTCAAATTTATCATGTAGGTCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAAt                                    <  5:321679/149‑1 (MQ=255)
                                                                                                        gcgATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAAt                                    >  1:107396/1‑149 (MQ=255)
                                                                                                         cgATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCaa                                       >  1:117580/1‑145 (MQ=255)
                                                                                                         cgATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCaa                                       <  2:117580/145‑1 (MQ=255)
                                                                                                            ttATGGGCAGCTTCTTCGTCAAATTTATCATGTAGCGCATCCAAACCGCTCTCGCGTATCACGTCGTCGTAGCTATTCGCCACATGATGATGGATTTTGGCTATCTGGAACAAACATTCGAAGCGCGTAAACGCTCCGCCAAAAtcta                                 >  1:213728/1‑147 (MQ=255)
                                                                                                               tGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCttt                             <  8:362461/149‑1 (MQ=255)
                                                                                                               tGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCtt                              <  6:257373/148‑1 (MQ=255)
                                                                                                               tGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCtt                              <  8:240664/148‑1 (MQ=255)
                                                                                                                gggCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCttt                             <  5:380719/148‑1 (MQ=255)
                                                                                                                     gCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGttatt                       <  5:149570/149‑1 (MQ=255)
                                                                                                                      cttcttCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGttatt                       >  7:148286/1‑148 (MQ=255)
                                                                                                                       ttcttcGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTAc                     >  8:75385/1‑149 (MQ=255)
                                                                                                                         cttcGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGTAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTAc                     <  2:232712/147‑1 (MQ=255)
                                                                                                                         cttcGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTg                   >  8:20397/1‑149 (MQ=255)
                                                                                                                          ttcGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCAcgtcg                                                                                                                   >  4:338468/1‑52 (MQ=255)
                                                                                                                          ttcGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCAcgtcg                                                                                                                   <  3:338468/52‑1 (MQ=255)
                                                                                                                          ttcGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGt                  >  1:216548/1‑149 (MQ=255)
                                                                                                                             gTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGt               >  7:377591/1‑149 (MQ=255)
                                                                                                                             gTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGt               >  4:226232/1‑149 (MQ=255)
                                                                                                                              tCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTg              >  3:358486/1‑149 (MQ=255)
                                                                                                                              tCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTAGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTg              >  5:1036/1‑149 (MQ=255)
                                                                                                                               cAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTGc             >  2:17774/1‑149 (MQ=255)
                                                                                                                                  aTTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTGCttt          >  7:252336/1‑149 (MQ=255)
                                                                                                                                   tttATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTGCTTTc         <  3:311373/149‑1 (MQ=255)
                                                                                                                                     tATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTGCTTTCa        <  2:22786/148‑1 (MQ=255)
                                                                                                                                     tATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTGCTTTCa        <  8:377591/148‑1 (MQ=255)
                                                                                                                                         aTGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTACTTTGTTATTACTGTCGTGCTTTCACTtct   >  2:357431/1‑149 (MQ=255)
                                                                                                                                          tgtaGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTGCTTTCACTtctc  >  2:382040/1‑149 (MQ=255)
                                                                                                                                          tgtaGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTGCTTTCACTtctc  >  7:221718/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                 
TTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTGCTTTCACTTCTC  >  NC_000913/755259‑755545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: