Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 4,001,645 Δ5 bp coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 4001645 4001649 5 34 [0] [0] 34 corA magnesium/nickel/cobalt transporter

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 40016440 (0.000)34 (0.910) 32/262 0.3 100% coding (219/951 nt) corA magnesium/nickel/cobalt transporter
?NC_000913 4001650 = 0 (0.000)coding (225/951 nt) corA magnesium/nickel/cobalt transporter

TATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGC  >  NC_000913/4001505‑4001781
                                                                                                                                                 |                                                                                                                                   
tgcagtatggattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTcc                                                                                                                                  <  6:63043‑M2/4‑1 (MQ=255)
               tcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTg                                                                                                                     >  7:147129‑M2/131‑147 (MQ=255)
                            gacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATc                                                                                                      <  8:147129‑M2/32‑1 (MQ=255)
                                  gacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTcc                                                                                                                                  <  7:50869‑M2/4‑1 (MQ=255)
                                  gacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTcc                                                                                                                                  >  8:50869‑M2/112‑115 (MQ=255)
                                       gcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAg                                                                                                         <  6:163880‑M2/29‑1 (MQ=255)
                                       gcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAg                                                                                                         >  5:163880‑M2/107‑135 (MQ=255)
                                            tgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCAc                                                                                      >  5:66871‑M2/102‑149 (MQ=255)
                                              cgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACt                                                                                     <  4:34780‑M2/49‑1 (MQ=255)
                                                cgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACtgtg                                                                                  <  6:66871‑M2/52‑1 (MQ=255)
                                                      atctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCAtt                                                                             >  8:107467‑M2/92‑148 (MQ=255)
                                                       tctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTa                                                                           >  8:3069‑M2/91‑149 (MQ=255)
                                                        ctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCGGGTAACTCCACTGTGGCATTTAc                                                                          >  3:120045‑M2/90‑149 (MQ=255)
                                                           aacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTAc                                                                          <  7:3069‑M2/60‑1 (MQ=255)
                                                                   cagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATg                                                               >  1:91611‑M2/79‑149 (MQ=255)
                                                                     gagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGgt                                                             <  4:120045‑M2/73‑1 (MQ=255)
                                                                          tggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCa                                                                                       >  4:103809‑M2/72‑118 (MQ=255)
                                                                          tggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCa                                                                                       <  3:103809‑M2/47‑1 (MQ=255)
                                                                                     ccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTcc                                                                                                                                  >  8:43682‑M2/61‑64 (MQ=255)
                                                                                     ccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTcc                                                                                                                                  <  7:43682‑M2/4‑1 (MQ=255)
                                                                                              gaagacatcgaagcatcggcacggttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgcgtgagcgt                                     <  7:107467‑M2/97‑1 (MQ=255)
                                                                                                          gcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGc                              >  1:81862‑M2/40‑143 (MQ=255)
                                                                                                          gcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGc                              <  2:81862‑M2/104‑1 (MQ=255)
                                                                                                             tcggcacgtttctttgaagacgacgagggcctgcatCTCCTTCTTCTTGTTTGAAGATGCGGTTGATCACGCCGGTAACTCCACTGTGGCATTTACCTTCCGTGTTGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTc                     <  7:106285‑M2/113‑1 (MQ=255)
                                                                                                                    gtttctttgaagacgccgacggcctgcttCTCCTTCTTCTTCTTTGAAGATGCGGATGATAACGCCGGTAAGTCCACTGTGTCATTTACCATCCGTGATGGTCGTCTGTTTACTCTg                                              <  6:117389‑M2/88‑1 (MQ=255)
                                                                                                                    gtttctttgaagacgacgacggactgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTCTGGCATTAACCATCCCTGATGGTCCTCTGTTTACTCTg                                              >  5:117389‑M2/30‑117 (MQ=255)
                                                                                                                     tttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTAt             >  4:87372‑M2/29‑149 (MQ=255)
                                                                                                                             aagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTc                  >  8:65411‑M2/21‑136 (MQ=255)
                                                                                                                             aagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTc                  <  7:65411‑M2/116‑1 (MQ=255)
                                                                                                                              agacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgcgtga                                         >  7:61605‑M2/20‑112 (MQ=255)
                                                                                                                              agacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgcgtga                                         <  8:61605‑M2/93‑1 (MQ=255)
                                                                                                                              agacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGt    <  2:91611‑M2/130‑1 (MQ=255)
                                                                                                                                acgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGCTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGc  >  7:22866‑M2/18‑149 (MQ=255)
                                                                                                                                                tCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCt                 >  6:94899‑M2/2‑118 (MQ=255)
                                                                                                                                                 |                                                                                                                                   
TATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGC  >  NC_000913/4001505‑4001781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.