Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,630,189 C→T 5.2% G242D (GGT→GAT)  ydfI ← putative NAD‑dependent D‑mannonate oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,630,1890CT 5.2% 242.4 / 2.5 76G242D (GGT→GAT) ydfIputative NAD‑dependent D‑mannonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (33/39);  new base T (2/2);  total (35/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

CATGCGCAACTTCATCTCTTCATAAGGCAGCACATCGCTAACCAGTTCGGCTCCCGCTTTTTCCCATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGTCAC  >  NC_000913/1630041‑1630336
                                                                                                                                                    |                                                                                                                                                   
cATGCGCAACTTCATCTCTTCATAAGGCAGCACATCGCTAACCAGTTCGGCTCCCGCTTTTTCCCATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCacac                                                                                                                                                     <  6:237754/149‑1 (MQ=255)
  tGCGCAACTTCATCTCTTCATAAGGCAGCACATCGCTAACCAGTTCGGCTCCCGCTTTTTCCCATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCg                                                                                                                                                   <  1:275204/149‑1 (MQ=255)
          ttCATCTCTTCATAAGGCAGCACATCGCTAACCAGATCGGCTCCCGCTTTTTCCCATTCCGGACTACCGGCAACAAAGTTAACTACTATTACCCACTGGCGGAAAGGTTCACAGGCAACTCCCGCAGTATCGCTCACACCGGGAAgttg                                                                                                                                           >  6:292727/1‑149 (MQ=255)
            cATCTCTTCATAAGGCAGCACATCGCTAACCAGTTCGGCTCCCGCTTTTTCCCATTCCGGACGTCCGGCAACAAAGTTATCTTCTGTTACCCACTGGCGGAAAGGTTCACATGCAACGCCCGCAGGATCGCGCACACCGGTAAg                                                                                                                                              <  1:47076/144‑1 (MQ=255)
            cATCTCTTCATAAGGCAGCACATCGCTAACCAGTTCGGCTCCCGCTTTTTCCCATTCCGGACGTCCGGCAACAAAGTTATCTTCTGTTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCTCACACCCGTAAgtagaa                                                                                                                                         >  2:47076/1‑145 (MQ=255)
            cATCTCTTCATAAGGCAGCACATCGCTAACCAGTTCGGCTACCGCTTTTTCCCATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCATCGCCCGCAGGATCGCGCACACCGGTAAgttgt                                                                                                                                          <  2:335095/148‑1 (MQ=255)
                  ttCATAAGGCAGCACATCGCTAACCAGTTCGGCTCCCGCTTTTTCCCATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGAttt                                                                                                                                   >  8:107092/1‑149 (MQ=255)
                     aTAAGGCAGCACATCGCTAACCAGTTCGGCTCCCGCTTTTTCCCATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTc                                                                                                                                 <  1:52977/148‑1 (MQ=255)
                     aTAAGGCAGCACATCGCTAACCAGTTCGGCTCCCGCTTTTTCCCATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTc                                                                                                                                 <  1:39300/148‑1 (MQ=255)
                     aTAAGGCAGCACATCGCTAACCAGTTCGGCTCCCGCTTTTTCCCATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCg                                                                                                                                >  2:87689/1‑149 (MQ=255)
                         ggCAGCACATCGCTAACCAGTTCGGCTCCCGCTTTTTCCCATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAg                                                                                                                            <  4:195912/149‑1 (MQ=255)
                          gcagcaCATCGCTAACCAGTTCGGCTCCCGCTTTTTCCCATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGc                                                                                                                           <  7:73716/149‑1 (MQ=255)
                            agcaCATCGCTAACCAGTTCGGCTCCCGCTTTTTCCCATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGc                                                                                                                           >  1:203869/1‑147 (MQ=255)
                            agcaCATCGCTAACCAGTTCGGCTCCCGCTTTTTCCCATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGc                                                                                                                           <  2:203869/147‑1 (MQ=255)
                            agcaCATCGCTAACCAGTTCGGCTCCCGCTTTTTCACATTCCGGACGTCCGGCAACAAAGTTATCTACTATTACCCACTCTCCGAAAGGTACACAGGCAACGCCCCAAGGATCGCGCACACCGGAAACATGTTCGATTTTCGCCAGCg                                                                                                                          >  8:4949/1‑148 (MQ=255)
                             gcaCATCGCTAACCAGTTCGGCTCCCGCTTTTTCCCATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGt                                                                                                                         >  2:319636/1‑148 (MQ=255)
                                 aTCGCTAACCAGTTCGGCTCCCGCTTTTTCCCATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCct                                                                                                                    >  1:161597/1‑149 (MQ=255)
                                      tAACCAGTTCGGCTCCCGCTTTTTCCCATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGt                                                                                                                >  2:299345/1‑148 (MQ=255)
                                        aCCAGTTCGGCTCCCGCTTTTTCCCATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCAc                                                                                                             >  1:379946/1‑149 (MQ=255)
                                           aGTTCGGCTCCCGCTTTTTCCCATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACATCGGTAAGTTGTTCGAtt                                                                                                                                    >  5:21628/1‑123 (MQ=255)
                                           aGTTCGGCTCCCGCTTTTTCCCATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACATCGGTAAGTTGTTCGAtt                                                                                                                                    <  6:16170/123‑1 (MQ=255)
                                           aGTTCGGCTCCCGCTTTTTCCCATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACATCGGTAAGTTGTTCGAtt                                                                                                                                    >  5:16170/1‑123 (MQ=255)
                                           aGTTCGGCTCCCGCTTTTTCCCATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACATCGGTAAGTTGTTCGAtt                                                                                                                                    <  6:21628/123‑1 (MQ=255)
                                             ttCGGCTCCCGCTTTTTCCCATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTGACTGCgg                                                                                                        <  7:14468/149‑1 (MQ=255)
                                              tCGGCTCCCGCTTTTTCCCATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCgt                                                                                                        <  4:125263/148‑2 (MQ=255)
                                                ggCTCCCGCTTTTTCCCATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCACCGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGca                                                                                                     >  2:304207/1‑149 (MQ=255)
                                                     ccGCTTTTTCCCATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACaa                                                                                                  <  7:107092/147‑1 (MQ=255)
                                                     ccGCTTTTTCCCATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATa                                                                                                >  1:318306/1‑149 (MQ=255)
                                                       gCTTTTTCCCATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACg                                                                                              <  8:139319/149‑1 (MQ=255)
                                                         tttttCCCATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGt                                                                                            >  8:193573/1‑149 (MQ=255)
                                                           tttCCCATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTc                                                                                           <  7:4949/148‑1 (MQ=255)
                                                               ccATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACgg                                                                                             >  8:92156/1‑142 (MQ=255)
                                                               ccATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACgg                                                                                             <  7:92156/142‑1 (MQ=255)
                                                                cATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCAt                                                                                     >  2:208535/1‑149 (MQ=255)
                                                                 aTTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCAtt                                                                                    >  4:24513/1‑149 (MQ=255)
                                                                      ggACGTCCGGCAACAAAGTTATCTTCTATTACCCACTTGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGa                                                                               >  4:97451/1‑149 (MQ=255)
                                                                        aCGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATg                                                                             <  7:253694/149‑1 (MQ=255)
                                                                         cGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTTACTGCGGGCACAATACGGTCCACCATTGTTGATgg                                                                            <  8:254333/149‑1 (MQ=255)
                                                                             cGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCacac                                                                                                                                                     >  5:385792/1‑72 (MQ=255)
                                                                             cGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCacac                                                                                                                                                     <  6:385792/72‑1 (MQ=255)
                                                                             cGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGaaa                                                                        <  1:319636/149‑1 (MQ=255)
                                                                                caacaaAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTc                                                                     <  1:299345/149‑1 (MQ=255)
                                                                                  acaaAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCTGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCCCTGCGGGCACAATACGGTCCACCATTGTTGATGGGCAAGTccc                                                                   >  4:251124/1‑147 (MQ=255)
                                                                                  acaaAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGt                                                                                                                >  8:219228/1‑104 (MQ=255)
                                                                                  acaaAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGt                                                                                                                <  7:219228/104‑1 (MQ=255)
                                                                                   caaAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACg                                                                  >  3:396733/1‑149 (MQ=255)
                                                                                        ttATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATc                                                                                                                      <  5:355168/92‑1 (MQ=255)
                                                                                        ttATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATc                                                                                                                      >  6:355168/1‑92 (MQ=255)
                                                                                           tcttctATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCtt                                                           <  1:68104/148‑1 (MQ=255)
                                                                                           tcttctATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCtt                                                           <  1:208535/148‑1 (MQ=255)
                                                                                               ctATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCAc                                                                                                             >  4:237351/1‑94 (MQ=255)
                                                                                               ctATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCAc                                                                                                             <  3:237351/94‑1 (MQ=255)
                                                                                                tATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATc                                                      >  5:348802/1‑148 (MQ=255)
                                                                                                  ttACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCa                                                    <  1:304207/148‑1 (MQ=255)
                                                                                                  ttACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCAt                                                   <  3:257526/149‑1 (MQ=255)
                                                                                                     cccACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGAt                                                                              >  6:110730/1‑119 (MQ=255)
                                                                                                     cccACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGAt                                                                              <  5:110730/119‑1 (MQ=255)
                                                                                                     cccACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATtgt                                                <  1:87689/149‑1 (MQ=255)
                                                                                                            gCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGtt                                         >  4:362262/1‑149 (MQ=255)
                                                                                                               gAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTa                                      <  3:24513/149‑1 (MQ=255)
                                                                                                               gAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTa                                      <  2:161597/149‑1 (MQ=255)
                                                                                                                    gTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATc                                  >  7:213039/1‑148 (MQ=255)
                                                                                                                           ggCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCtt                          <  8:213039/149‑1 (MQ=255)
                                                                                                                              aaCGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGaaa                                                                        >  5:61660/1‑100 (MQ=255)
                                                                                                                              aaCGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGaaa                                                                        <  6:61660/100‑1 (MQ=255)
                                                                                                                              aaCGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATAGTGCCAGTTTTACATCAACGGCTtgtg                       >  4:260519/1‑149 (MQ=255)
                                                                                                                               aCGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGc                      <  3:180209/149‑1 (MQ=255)
                                                                                                                                   ccGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAAc                               <  3:324538/136‑1 (MQ=255)
                                                                                                                                   ccGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAAc                               >  4:324538/1‑136 (MQ=255)
                                                                                                                                   ccGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAg                  <  7:193573/149‑1 (MQ=255)
                                                                                                                                   ccGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAg                  >  6:382228/1‑149 (MQ=255)
                                                                                                                                    cGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAgg                 <  2:379946/149‑1 (MQ=255)
                                                                                                                                        ggATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGt             >  7:406780/1‑149 (MQ=255)
                                                                                                                                           tCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGAt                                                                              >  2:274795/1‑81 (MQ=255)
                                                                                                                                           tCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGAt                                                                              <  1:274795/81‑1 (MQ=255)
                                                                                                                                             gcgcACCCCGGTCAGTTGTTCGATGTTCGCCAGCGTATCCTCTGGCCCTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGGCCCGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACaa        <  6:348802/149‑1 (MQ=255)
                                                                                                                                              cgcACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGttt                                        >  7:242864/1‑116 (MQ=255)
                                                                                                                                              cgcACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGttt                                        <  8:242864/116‑1 (MQ=255)
                                                                                                                                                cacaCCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGt     <  3:362262/149‑1 (MQ=255)
                                                                                                                                                 acacCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGt     >  1:221492/1‑148 (MQ=255)
                                                                                                                                                   acCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATAGGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTAGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGTCAc  >  6:62048/1‑149 (MQ=255)
                                                                                                                                                   acCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATtgt                                                >  1:317001/1‑103 (MQ=255)
                                                                                                                                                   acCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATtgt                                                <  2:317001/103‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CATGCGCAACTTCATCTCTTCATAAGGCAGCACATCGCTAACCAGTTCGGCTCCCGCTTTTTCCCATTCCGGACGTCCGGCAACAAAGTTATCTTCTATTACCCACTGGCGGAAAGGTTCACAGGCAACGCCCGCAGGATCGCGCACACCGGTAAGTTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGTCAC  >  NC_000913/1630041‑1630336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: