Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913127,5401.C69.2% 57.9 / 26.8 26coding (1846/1893 nt)aceFpyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
Reads supporting (aligned to +/- strand):  ref base . (4/4);  new base C (10/8);  total (14/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GCGCCGGAAGTGGCTATCCTCGGCGTTTCCAAGTCCGCGATGGAGCCGGTGTGGAATGGTAAAGAGTTCGTGCCGCGTCTGATGCTGCCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGAC  >  NC_000913/127393‑127661
                                                                                                                                                    |                                                                                                                         
gcgcCGGAAGTGGCTATCCTCGGCGTTTCCAAGTCCGCGATGGAGCCGGTGTGGAATGGTAAAGAGTTCGTGCCGCGTCTGATGCTGCCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTAc                                                                                                                          <  1:87647/149‑1 (MQ=255)
          tGGCTATCCTCGGCGTTTCCAAGTCCGCGATGGAGCCGGTGTGGAATGGTAAAGAGTTCGTGCCGCGTCTGATGCTGCCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTaa                                                                                                                 >  8:130007/1‑148 (MQ=255)
              tATCCTCGGCGTTTACAAGTCCGCGATGGAGCCGGTGTGGAATTGTAAAGAGTTAGTGCCGCGGCTGATGCTGCCGATTTCTCTATACTTAGACCACCCCGTGATCGACGGTGCTGATGGTGCCCCTTTCATTAc                                                                                                                          >  4:5383/1‑135 (MQ=255)
              tATCCTCGGCGGTTCCAAGTCCGCGATGGAGCCGGTGTGGAATGGTAAAGAGTTCGTGCCGCGTCTGATGCTGCCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTAc                                                                                                                          <  3:5383/135‑1 (MQ=255)
                     ggCGTTTCCAAGTCCGCGATGGAGCCGGTGTGGAATGGTAAAGAGTTCGTGCCGCGTCTGATGCTGCCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCCATCATTAACAACACGCt                                                                                                        <  6:107260/147‑1 (MQ=255)
                          ttCCAAGTCCGCGATGGAGCCGGTGTGGAATGGTAAAGAGTTCGTGCCGCGTCTGATGCTGCCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCCATCATTaac                                                                                                                >  1:167016/1‑134 (MQ=255)
                          ttCCAAGTCCGCGATGGAGCCGGTGTGGAATGGTAAAGAGTTCGTGCCGCGTCTGATGCTGCCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCCATCATTaac                                                                                                                <  2:167016/134‑1 (MQ=255)
                          ttCCAAGTCCGCGATGGAGCCGGTGTGGAATGGTAAAGAGTTCGTGCCGCGTCTGATGCTGCCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCCATCATTAACAACACGCTGTCTGa                                                                                                  >  1:58015/1‑148 (MQ=255)
                                      gATGGAGCCGGTGTGGAATGGTAAAGAGTTCGTGCCGCGTCTCATGCTGCCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTgg                                                                                    <  7:130007/149‑1 (MQ=255)
                                           aGCCGGTGTGGAATGGTAAAGAGTTCGTGCCGCGTCTGATGCTGCCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATg                                                                               >  1:164733/1‑149 (MQ=255)
                                                     gAATGGTAAAGAGTTCGTGCCGCGTCTGATGCTGCCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATgtaagtaa                                                                        >  3:51023/1‑147 (MQ=255)
                                                         ggTAAAGAGTTCGTGCCGCGTCTGATGCTGCCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATgt                                                                              <  1:159388/136‑1 (MQ=255)
                                                         ggTAAAGAGTTCGTGCCGCGTCTGATGCTGCCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATgt                                                                              >  2:159388/1‑136 (MQ=255)
                                                                     gTGCCGCGTCTGATGCTGCCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAAcggc                                                      >  5:103415/1‑149 (MQ=255)
                                                                                  tgctgcCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAAAGCCGGCCGTTGGGCCGGCtcttttactt                                        <  2:164733/149‑5 (MQ=255)
                                                                                      gcCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAAcggccg                                                    >  3:22124/1‑134 (MQ=255)
                                                                                      gcCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAAcggccg                                                    <  4:22124/134‑1 (MQ=255)
                                                                                                         gACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAg                   <  8:91045/148‑1 (MQ=255)
                                                                                                          accaccGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGt                 >  2:129422/1‑149 (MQ=255)
                                                                                                            caccGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTa                        >  8:97947/1‑140 (MQ=255)
                                                                                                            caccGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTa                        <  7:97947/140‑1 (MQ=255)
                                                                                                              ccGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGa                    <  5:4189/142‑1 (MQ=255)
                                                                                                              ccGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGa                    >  6:4189/1‑142 (MQ=255)
                                                                                                               cgcgGGATCGACGGGGCTGAGGGTGCCCGTTGCATTACCCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGttatta            <  6:103415/149‑1 (MQ=255)
                                                                                                                  gTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCa                                >  1:7068/1‑126 (MQ=255)
                                                                                                                  gTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCa                                <  2:7068/126‑1 (MQ=255)
                                                                                                                     aTCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGaa       <  4:22460/147‑1 (MQ=255)
                                                                                                                     aTCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGaa       >  3:22460/1‑147 (MQ=255)
                                                                                                                          cGGTGCTGATGGTGCCCGTTTCATTACCCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGAc  >  2:21067/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                         
GCGCCGGAAGTGGCTATCCTCGGCGTTTCCAAGTCCGCGATGGAGCCGGTGTGGAATGGTAAAGAGTTCGTGCCGCGTCTGATGCTGCCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGAC  >  NC_000913/127393‑127661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: