Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,662,350 C→T P599S (CCC→TCC)  ynfF → S‑ and N‑oxide reductase, A subunit, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,662,3500CT100.0% 148.6 / NA 44P599S (CCC→TCC) ynfFS‑ and N‑oxide reductase, A subunit, periplasmic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/21);  total (23/21)

ACGCAAACCCATCTACTGGATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTC  >  NC_000913/1662208‑1662489
                                                                                                                                              |                                                                                                                                           
aCGCAAACCCATCTACTGGATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGAc                                                                                                                                        >  1:132907/1‑148 (MQ=255)
                    tGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGAcc                                                                                                                   <  6:74963/149‑1 (MQ=255)
                       tGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCgg                                                                                                                 >  6:75427/1‑148 (MQ=255)
                       tGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCgg                                                                                                                 >  3:127813/1‑148 (MQ=255)
                           tGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCt                                                                                                            <  5:75427/149‑1 (MQ=255)
                                      aaCGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGAcc                                                                                                                   >  8:43863/1‑131 (MQ=255)
                                      aaCGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGAcc                                                                                                                   <  7:43863/131‑1 (MQ=255)
                                                  cAGACGTTTATAAAACCTTTACTGACGGTCGAAGTCTGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGTCTGGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGgaagaa                                                                                     <  2:132907/149‑1 (MQ=255)
                                                    gACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGcc                                                                                             >  1:116751/1‑139 (MQ=255)
                                                    gACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGcc                                                                                             <  2:116751/139‑1 (MQ=255)
                                                        tttATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAg                                                                                                       <  6:20051/125‑1 (MQ=255)
                                                        tttATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAg                                                                                                       >  5:20051/1‑125 (MQ=255)
                                                            tCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCg                                                                           >  6:113921/1‑149 (MQ=255)
                                                                aCCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCttt                                                                       <  4:127813/149‑1 (MQ=255)
                                                                   tttACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGaaa                                                                                                    >  2:124058/1‑117 (MQ=255)
                                                                   tttACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGaaa                                                                                                    <  1:124058/117‑1 (MQ=255)
                                                                     tACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCcgc                                                                   <  5:113921/148‑1 (MQ=255)
                                                                              tCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGt                                                          >  6:94376/1‑148 (MQ=255)
                                                                                         aTGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGAcc                                                                                                                   <  5:39844/80‑1 (MQ=255)
                                                                                         aTGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGAcc                                                                                                                   >  6:39844/1‑80 (MQ=255)
                                                                                              tGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCaaa                                         >  5:26925/1‑149 (MQ=255)
                                                                                               ggATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAAc                                        >  2:101408/1‑149 (MQ=255)
                                                                                                gATCAAATATCTCCATGCGAAAACGAAGGAGCGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCaa                                          <  2:13251/146‑1 (MQ=255)
                                                                                                gATCAAATATCTCCATGCGAAAACGAAGGAGCGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCaa                                          >  1:13251/1‑146 (MQ=255)
                                                                                                  tCATATATGTCCATTCGTATATGAAGGAACGTAACCCTGAGATGTCCGACTACGATGTGATGAAAACGTCAGGGATCTTTAATAAAAAATGCCCGGTAGAACACTACGTCTCTTTCCGCTCATTCCGTGa                                                        <  6:85625/130‑1 (MQ=255)
                                                                                                  tCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCAGACTACGAGGAGATGAAAAAGACCGGTATCTTTAAGAAAAAATGACCGGAAGAACAAAACTTCGCTTTCAGCGCATTCCGTGa                                                        >  5:85625/1‑130 (MQ=255)
                                                                                                    aaaTATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGtt                                   <  5:94376/149‑1 (MQ=255)
                                                                                                     aaTATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGtt                                   >  4:289/1‑148 (MQ=255)
                                                                                                     aaTATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGtt                                   >  2:73812/1‑148 (MQ=255)
                                                                                                      atatCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGccc                                                                                            >  1:136317/1‑90 (MQ=255)
                                                                                                      atatCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGccc                                                                                            <  2:136317/90‑1 (MQ=255)
                                                                                                      atatCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGa                                 <  6:26925/149‑1 (MQ=255)
                                                                                                                          aGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATc             <  1:101408/149‑1 (MQ=255)
                                                                                                                               cGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAAt        <  3:289/149‑1 (MQ=255)
                                                                                                                                gTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCcgc                                                                   >  6:149204/1‑89 (MQ=255)
                                                                                                                                gTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCcgc                                                                   <  5:149204/89‑1 (MQ=255)
                                                                                                                                 tAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCcaca                                               >  6:66328/1‑108 (MQ=255)
                                                                                                                                 tAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCcaca                                               <  5:66328/108‑1 (MQ=255)
                                                                                                                                   aCCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGtt                                   >  6:24005/1‑118 (MQ=255)
                                                                                                                                   aCCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGtt                                   <  5:24005/118‑1 (MQ=255)
                                                                                                                                     ccTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTc  >  1:61188/1‑149 (MQ=255)
                                                                                                                                      cTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGa                                 >  3:51284/1‑117 (MQ=255)
                                                                                                                                      cTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGa                                 <  4:51284/117‑1 (MQ=255)
                                                                                                                                       tGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGaaaa               >  1:179502/1‑134 (MQ=255)
                                                                                                                                              |                                                                                                                                           
ACGCAAACCCATCTACTGGATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTC  >  NC_000913/1662208‑1662489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑