Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,806,319	C→G	intergenic (‑236/‑51)	ydiY ← / → pfkB	acid‑inducible putative outer membrane protein/6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,806,319	0	C	G	100.0%	121.6 / NA	35	intergenic (‑236/‑51)	ydiY/pfkB	acid‑inducible putative outer membrane protein/6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (16/19);  total (16/19)

TTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTA  >  NC_000913/1806177‑1806460                                                                                                                                               |                                                                                                                                              ttATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTc                                                                                                                                         >  7:27961/1‑149 (MQ=255)      aaaaGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCt                                                                                                                                     <  3:15422/148‑1 (MQ=255)                      ttAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACt                                                                                                                     >  2:109201/1‑148 (MQ=255)                      ttAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACt                                                                                                                     >  2:106600/1‑148 (MQ=255)                           aaTGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAAt                                                                                                                >  8:43299/1‑148 (MQ=255)                               tATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGc                                                                                                            <  1:106600/148‑1 (MQ=255)                               tATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGc                                                                                                            <  1:109201/148‑1 (MQ=255)                                aTTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCCGcc                                                                                                           >  4:83520/1‑148 (MQ=255)                                aTTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGATCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGcc                                                                                                           >  4:81614/1‑148 (MQ=255)                                       tttCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATaggag                                                                                                   >  1:86981/1‑149 (MQ=255)                                           aTTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGaaa                                                                                               <  2:86981/149‑1 (MQ=255)                                                       aGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGt                                                                                    <  2:102046/148‑1 (MQ=255)                                                                              tatttatATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGccc                                                            >  5:18595/1‑149 (MQ=255)                                                                                  tatatataTTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCctct                                                         <  1:41885/148‑1 (MQ=255)                                                                                     atatatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCtctctc                                                      >  4:72860/1‑148 (MQ=255)                                                                                         atTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGtt                                                                         <  1:51108/125‑1 (MQ=255)                                                                                         atTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGtt                                                                         >  2:51108/1‑125 (MQ=255)                                                                                                tGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTa                                                                                       >  3:77169/1‑104 (MQ=255)                                                                                                tGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTa                                                                                       <  4:77169/104‑1 (MQ=255)                                                                                                             ataaaGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCaaa                             <  6:18595/149‑1 (MQ=255)                                                                                                                   cTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACtt                                                                  >  2:35461/1‑106 (MQ=255)                                                                                                                   cTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACtt                                                                  <  1:38055/106‑1 (MQ=255)                                                                                                                   cTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACtt                                                                  <  1:35461/106‑1 (MQ=255)                                                                                                                   cTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACtt                                                                  >  2:38055/1‑106 (MQ=255)                                                                                                                      cAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTAc                                                                                      <  6:60765/83‑1 (MQ=255)                                                                                                                                  ttGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTgc        <  3:83520/149‑1 (MQ=255)                                                                                                                                  ttGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTgc        <  3:81614/149‑1 (MQ=255)                                                                                                                                   tGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTAt                                                                                  >  2:28202/1‑74 (MQ=255)                                                                                                                                   tGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTAt                                                                                  <  1:28202/74‑1 (MQ=255)                                                                                                                                        aTTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGtt                                                                         <  5:12771/78‑1 (MQ=255)                                                                                                                                        aTTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGtt                                                                         >  6:12771/1‑78 (MQ=255)                                                                                                                                        aTTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTg         <  4:15408/142‑1 (MQ=255)                                                                                                                                        aTTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTg         >  3:15408/1‑142 (MQ=255)                                                                                                                                        aTTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGt   <  7:43299/148‑1 (MQ=255)                                                                                                                                        aTTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTa  <  8:27961/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                              TTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTA  >  NC_000913/1806177‑1806460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑