Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,091,644 G→T 6.1% G241G (GGC→GGA frvB ← putative PTS enzyme, IIB component/IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,091,6440GT 6.1% 222.2 / 3.2 66G241G (GGC→GGAfrvBputative PTS enzyme, IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base G (35/27);  new base T (2/2);  total (37/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

GCCCATGAGGTCGGACATCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCA  >  NC_000913/4091497‑4091792
                                                                                                                                                   |                                                                                                                                                    
gcccATGAGGTCGGACATCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGc                                                                                                                                                     >  3:285849/1‑149 (MQ=255)
 cccATGAGGTCGGACATCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTTATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGcc                                                                                                                                                    <  2:79696/149‑1 (MQ=255)
   cATGAGGTCGGACATCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCaa                                                                                                                                                  <  2:294193/149‑1 (MQ=255)
            ggccATCACCGGCCCGATAACGTAGTAAGTGAGCACGGCGAAAACGAGCAGAGTAACGAACTGGATCAACATTGCACCGAGCAGCGGGTGTAAAGCTTTGCCAAGGCGCCCGTTGCGGACCCAGACGCCGTATTAGGCCGTCGGCAGcc                                                                                                                                         >  2:159223/4‑149 (MQ=255)
            ggACATCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGc                                                                                                                                          <  3:2963/148‑1 (MQ=255)
                 tCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAg                                                                                                                                     >  3:60574/1‑148 (MQ=255)
                                     taAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATATCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATcc                                                                                                                >  3:66646/1‑149 (MQ=255)
                                     taAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATATCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATcc                                                                                                                >  7:157183/1‑149 (MQ=255)
                                       aGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATccc                                                                                                               <  5:232738/148‑1 (MQ=255)
                                        gTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCg                                                                                                              >  7:63344/1‑148 (MQ=255)
                                         tGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCa                                                                                                            <  7:55453/149‑1 (MQ=255)
                                            gCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCAccc                                                                                                         >  8:10047/1‑149 (MQ=255)
                                             cACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCAccc                                                                                                         >  5:158890/1‑148 (MQ=255)
                                                gCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACt                                                                                                     >  7:177660/1‑149 (MQ=255)
                                                  cGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCATTCGCCAGCCCCAGCCCCACCGCGCCGAGGAATCCCGCACCCGACTgg                                                                                                   >  2:348180/1‑149 (MQ=255)
                                                  cGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTgg                                                                                                   >  8:147641/1‑149 (MQ=255)
                                                  cGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTgg                                                                                                   <  2:10646/149‑1 (MQ=255)
                                                  cGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTgg                                                                                                   >  3:307219/1‑149 (MQ=255)
                                                   gAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCTGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTggg                                                                                                  <  5:129467/149‑1 (MQ=255)
                                                   gAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTggg                                                                                                  >  8:51730/1‑149 (MQ=255)
                                                         cagcagAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGcc                                                                                              >  4:303520/1‑147 (MQ=255)
                                                          agcagAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGag                                                                                           >  4:60264/1‑149 (MQ=255)
                                                            catcgTAACGACCGGGCTCAGCAGTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGACGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGagca                                                                                         <  6:170282/145‑1 (MQ=255)
                                                            cagAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGagca                                                                                         <  2:317637/149‑1 (MQ=255)
                                                              gagTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGGGTTGCCAAGGCGCCCTTTGCGGATCCAGAAGACGAAAGAGCCAGTCGGGAGGGCGGGCACCACCGCGCGGGGGACTCCCGCGCCCGGCTGGGTGCCgcgccgg                                                                                       >  4:211844/1‑146 (MQ=255)
                                                                 tAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCt                                                                                    <  6:344454/149‑1 (MQ=255)
                                                                    cgaacgGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTg                                                                                 <  8:63344/149‑1 (MQ=255)
                                                                     gaacgGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGTCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGt                                                                                >  5:321754/1‑149 (MQ=255)
                                                                     gaacgGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGt                                                                                <  3:128848/149‑1 (MQ=255)
                                                                            aTCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCACTCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTctc                                                                         >  1:196653/1‑147 (MQ=255)
                                                                                gCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGa                                                                     <  3:60264/149‑1 (MQ=255)
                                                                                  aTTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGAt                                                                    <  4:60574/148‑1 (MQ=255)
                                                                                  aTTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATa                                                                   <  6:89737/149‑1 (MQ=255)
                                                                                   ttGAACCGAGCAGCGGTTGTAATGCTTTGCCATGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAg                                                                  >  3:201293/1‑149 (MQ=255)
                                                                                   ttGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAg                                                                  >  3:167912/1‑149 (MQ=255)
                                                                                        ccGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAAc                                                             <  3:211844/149‑1 (MQ=255)
                                                                                         cGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAAcc                                                            <  6:158890/149‑1 (MQ=255)
                                                                                          gAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCa                                                           <  5:174531/149‑1 (MQ=255)
                                                                                             cagcGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGTAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGaa                                                        <  2:56253/149‑1 (MQ=255)
                                                                                               gcGGTTGTACTGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTc                                                                           >  2:272473/1‑128 (MQ=255)
                                                                                               gcGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTc                                                                           <  1:272473/128‑1 (MQ=255)
                                                                                                      tAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGa                                                <  8:52586/148‑1 (MQ=255)
                                                                                                      tAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAg                                               >  4:320819/1‑149 (MQ=255)
                                                                                                       aaTGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGc                                              >  1:310112/1‑149 (MQ=255)
                                                                                                        aTGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGc                                              <  7:144836/148‑1 (MQ=255)
                                                                                                           cTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGa                                            >  3:162287/1‑147 (MQ=255)
                                                                                                           cTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGa                                            >  6:5577/1‑147 (MQ=255)
                                                                                                            tttGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGACGAGCAGCGCTTTGTCGTTCGCCAGATAGAAAACAAGAAACGCCGGAGCGAAAg                                         >  2:131041/1‑149 (MQ=255)
                                                                                                                 cAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAGCGCCGGAGCGAAAGCCGGt                                    <  7:10047/149‑1 (MQ=255)
                                                                                                                 cAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGt                                    >  4:236522/1‑149 (MQ=255)
                                                                                                                       gcACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGACACGCCGGAGCGACAGCCGGTTTATCg                              >  2:309189/1‑149 (MQ=255)
                                                                                                                        cACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCCCCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCgg                             <  3:250426/149‑1 (MQ=255)
                                                                                                                             tGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCgg                                                 <  2:262795/124‑1 (MQ=255)
                                                                                                                             tGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCgg                                                 >  1:262795/1‑124 (MQ=255)
                                                                                                                               cGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATcga                      >  4:26238/1‑149 (MQ=255)
                                                                                                                                  aaCCAGAAGACGAAATATCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATcgacga                   <  8:157183/149‑1 (MQ=255)
                                                                                                                                  aaCCAGAAGACGAAATATCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATcgacga                   <  4:66646/149‑1 (MQ=255)
                                                                                                                                   aCCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATcc                                                                                                                <  1:11403/55‑1 (MQ=255)
                                                                                                                                   aCCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATcc                                                                                                                >  2:11403/1‑55 (MQ=255)
                                                                                                                                     cAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAgg                 >  3:16510/1‑148 (MQ=255)
                                                                                                                                     cAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACACGACAGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAgg                 >  1:111722/1‑148 (MQ=255)
                                                                                                                                       gaagaCGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCaa              >  7:177092/1‑149 (MQ=255)
                                                                                                                                       gaagaCGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCaa              >  4:194136/1‑149 (MQ=255)
                                                                                                                                       gaagaCGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCATTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCaa              >  5:305592/1‑149 (MQ=255)
                                                                                                                                              aaaTAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAgc       >  7:85994/1‑149 (MQ=255)
                                                                                                                                               aaTAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAgcg      <  2:310112/149‑1 (MQ=255)
                                                                                                                                                   gCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCa  >  7:226668/1‑149 (MQ=255)
                                                                                                                                                   gCCAATCGACAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGGCGTTCTCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGccc   <  3:204688/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCCCATGAGGTCGGACATCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCA  >  NC_000913/4091497‑4091792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: