Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 169,748 T→A 5.3% intergenic (+21/‑30) fhuA → / → fhuC ferrichrome outer membrane transporter/iron(3+)‑hydroxamate import ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913169,7480TA 5.3% 290.6 / 3.8 92intergenic (+21/‑30)fhuA/fhuCferrichrome outer membrane transporter/iron(3+)‑hydroxamate import ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (40/45);  new base A (3/2);  total (45/47)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

GCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGAC  >  NC_000913/169600‑169896
                                                                                                                                                    |                                                                                                                                                    
gctCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGAt                                                                                                                                                      <  5:159510/149‑1 (MQ=255)
  tCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGAttt                                                                                                                                                    >  7:114059/1‑149 (MQ=255)
    catcGTGGCGCTGCATGTTAAGAACCTGTTCGATAGTGAATACGTCGCCAGCTGCTTTAACACTTTTGGCTGCTTCTGGGGCGCAGAACGTAAGGTGGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTcc                                                                                                                                                  <  5:255701/146‑1 (MQ=255)
     aaCGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGAAATCCg                                                                                                                                                 >  1:215491/1‑149 (MQ=255)
      acctGGCGCTGCATTTTAACAACCTGTTCGATCTTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGTGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGt                                                                                                                                                <  8:136718/146‑1 (MQ=255)
           gcgcTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCa                                                                                                                                           >  3:295729/1‑149 (MQ=255)
                  aTGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCg                                                                                                                                                 >  5:333693/1‑136 (MQ=255)
                  aTGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCg                                                                                                                                                 <  6:333693/136‑1 (MQ=255)
                   tGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTcac                                                                                                                                     >  6:266327/1‑147 (MQ=255)
                   tGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTcac                                                                                                                                     >  6:327461/1‑147 (MQ=255)
                   tGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACaa                                                                                                                                   <  3:51818/149‑1 (MQ=255)
                   tGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACaa                                                                                                                                   >  1:52827/1‑149 (MQ=255)
                     ttAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGt                                                                                                                                 >  7:25497/1‑149 (MQ=255)
                       aacaaaCTGTTCGATCTTGAATACGTAGCCAGATGCTTTAACACTTATGGCTGCTTATGGGGCGCAGAAAGTCAGGTCGTTGCAACCTCAAAATTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTTCCCATTTCACAAGtt                                                                                                                                <  1:121162/148‑1 (MQ=255)
                          aacCTTTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTTGGGCGAAGAACGTCAGGTCGTTGAAACCGCAACCTTCAGTTTCTAATTTATCTTTTGGGGCACGGATTTACGTGCCCAGTTACCAAGTTGTCt                                                                                                                            >  2:276254/1‑149 (MQ=255)
                            ccTGTTCGATCGTGAATACGGCGCCAGCTGCTTTAACACTGATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTGGCAACCGCAACCTGCCGGTTCTAATTTCTCTTTTGGGGCACGGAGTTCCGTGCCCATTTCACAAGTTGGCTGt                                                                                                                          >  4:72133/1‑149 (MQ=255)
                             cTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGtt                                                                                                                         <  8:114059/149‑1 (MQ=255)
                              tGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTa                                                                                                                        >  1:48550/1‑149 (MQ=255)
                              tGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTa                                                                                                                        >  1:299023/1‑149 (MQ=255)
                               gTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTAt                                                                                                                       <  6:4719/149‑1 (MQ=255)
                                ttCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGGTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATg                                                                                                                      >  4:24771/1‑149 (MQ=255)
                                ttCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATg                                                                                                                      >  1:342730/1‑149 (MQ=255)
                                ttCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCGGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATg                                                                                                                      <  4:18992/149‑1 (MQ=255)
                                   gATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCa                                                                                                                    <  3:24771/148‑1 (MQ=255)
                                   gATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAg                                                                                                                   >  8:332782/1‑149 (MQ=255)
                                   gATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAg                                                                                                                   <  2:325941/149‑1 (MQ=255)
                                   gATCGTGAATACGTCGCCAGCTGCCTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAg                                                                                                                   >  1:180670/1‑149 (MQ=255)
                                     tCGTGAATACGTCGCGATCTGCGTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGTTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGa                                                                                                                 <  8:137838/149‑1 (MQ=255)
                                     tCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGAAATCCGTGccccaaaa                                                                                                                                       <  2:224951/127‑6 (MQ=255)
                                     tCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGAAATCCGTGccccaaaa                                                                                                                                       >  1:224951/1‑122 (MQ=255)
                                      cGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGCGGCACGGAGTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGaa                                                                                                                >  2:124385/1‑149 (MQ=255)
                                       gTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAAt                                                                                                               <  7:241781/149‑1 (MQ=255)
                                         gAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATa                                                                                                              >  8:15947/1‑148 (MQ=255)
                                           aTACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATacac                                                                                                           <  7:29380/149‑1 (MQ=255)
                                            tACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATacac                                                                                                           >  1:289237/1‑148 (MQ=255)
                                               gTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGAGCAGAACGTCAGGTCGTTGCTACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAAt                                                                                                       <  7:273840/149‑1 (MQ=255)
                                                   ccAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCAtt                                                                                                   <  2:180670/149‑1 (MQ=255)
                                                    cAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGGAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCATGAATACACGAATCATTc                                                                                                  <  2:325451/149‑1 (MQ=255)
                                                       ctgctTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCTGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGa                                                                                               <  7:288625/149‑1 (MQ=255)
                                                          ctTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATAc                                                                                            <  7:332782/149‑1 (MQ=255)
                                                                cacTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACttt                                                                                      <  8:37238/149‑1 (MQ=255)
                                                                 acTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCg                                                                                                                                                 <  7:218851/89‑1 (MQ=255)
                                                                 acTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCg                                                                                                                                                 >  8:218851/1‑89 (MQ=255)
                                                                 acTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACtttt                                                                                     <  1:290073/149‑1 (MQ=255)
                                                                  cTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTg                                                                                    >  5:121846/1‑149 (MQ=255)
                                                                         ctgctTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCg                                                                             <  8:113214/149‑1 (MQ=255)
                                                                          tgctTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGa                                                                                               <  3:253505/130‑1 (MQ=255)
                                                                          tgctTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGa                                                                                               >  4:253505/1‑130 (MQ=255)
                                                                          tgctTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGAAACCACTTTTGCACTGCg                                                                             >  8:124412/1‑148 (MQ=255)
                                                                           gctTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGt                                                                            >  4:298812/1‑148 (MQ=255)
                                                                            ctTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTaa                                                                          <  5:266327/149‑1 (MQ=255)
                                                                             ttCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTaa                                                                          >  1:98884/1‑148 (MQ=255)
                                                                             ttCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTaa                                                                          >  3:187857/1‑148 (MQ=255)
                                                                             ttCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTaa                                                                          >  2:15031/1‑148 (MQ=255)
                                                                               cTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAatat                                                                       >  8:126113/1‑149 (MQ=255)
                                                                               cTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAata                                                                        <  8:230672/148‑1 (MQ=255)
                                                                                tGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATAtc                                                                      >  1:252269/1‑149 (MQ=255)
                                                                                  gggCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATAtct                                                                     <  2:209744/148‑1 (MQ=255)
                                                                                   ggCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTcc                                                                   <  2:52827/149‑1 (MQ=255)
                                                                                         gAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCgt                                                             >  1:127404/1‑149 (MQ=255)
                                                                                           aCGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCttgt                                                           <  3:298812/149‑1 (MQ=255)
                                                                                            cGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGCTTTCCGTGCCCATTTCACAAGTTGGCTGGTATGCAGGAATACACGAATCATTCAGATCCCACTTTTGCACTGCGTAATATCTCCGTTCgtggg                                                          >  8:288878/1‑147 (MQ=255)
                                                                                                 ggTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCgg                                                     <  7:59777/149‑1 (MQ=255)
                                                                                                  gTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCggg                                                    >  4:220617/1‑149 (MQ=255)
                                                                                                        gcaaccgcaaccTTCCGATTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCACGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCAc                                               <  1:202693/148‑1 (MQ=255)
                                                                                                         caaccgcaaccTTCCGTTTCTAATTTCTCTTTTGGGGCACGGTATTCCGTGCCCATTTCACTAGTTGGCTGTTATGCAGGAAGACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGc                                             <  1:124385/149‑1 (MQ=255)
                                                                                                         caaccgcaaccTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACg                                              <  7:15947/148‑1 (MQ=255)
                                                                                                           accgcaaccTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCtt                                           >  5:79499/1‑149 (MQ=255)
                                                                                                            ccgcaaccTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCttt                                          <  6:79499/149‑1 (MQ=255)
                                                                                                            ccgcaaccTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCAATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCttt                                          >  8:310900/1‑149 (MQ=255)
                                                                                                             cgcaaccTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCtttt                                         >  8:224040/1‑149 (MQ=255)
                                                                                                             cgcaaccTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCtttt                                         <  7:126113/149‑1 (MQ=255)
                                                                                                             cgcaaccTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCtttt                                         <  2:299023/149‑1 (MQ=255)
                                                                                                                    ttCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTACGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTGTTGCATCCg                                  >  7:336842/1‑149 (MQ=255)
                                                                                                                        gTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAata                                                                        <  5:92859/107‑1 (MQ=255)
                                                                                                                        gTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAata                                                                        >  6:92859/1‑107 (MQ=255)
                                                                                                                         tttCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTg                                                                               >  1:204536/1‑99 (MQ=255)
                                                                                                                         tttCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTg                                                                               <  2:204536/99‑1 (MQ=255)
                                                                                                                            cTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTAGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTCATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGt                           >  3:100994/1‑148 (MQ=255)
                                                                                                                             tAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTAGCACTGCGTAATATCTCCTTTCGTGTGACCGGGCGCACGCTTTTGCATCCGCTGTCGtt                          >  5:25526/1‑148 (MQ=255)
                                                                                                                               aTTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTaa                        <  2:98884/148‑1 (MQ=255)
                                                                                                                               aTTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAAc                       <  3:178131/149‑1 (MQ=255)
                                                                                                                                   ctctTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCtt                    <  2:127404/148‑1 (MQ=255)
                                                                                                                                    tctTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTACTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTc                  >  6:95496/1‑149 (MQ=255)
                                                                                                                                    gctTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATTCACGACTCATTCCGATACCACTTTTGCTCTGCGTAATATCTCCTTTCGTGCGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTc                  <  4:64174/148‑1 (MQ=255)
                                                                                                                                     ctTTTGGGGCACGGATTTCCGTGCCCATTTAACAAGTTGGCGGTTATGCAGGAATACCCGAATCATTCCGATACCACTTTTGCACTGCTTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTcc                 <  2:30064/149‑1 (MQ=255)
                                                                                                                                      ttttGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTc                  <  4:187857/147‑1 (MQ=255)
                                                                                                                                      ttttGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTc                  <  1:15031/147‑1 (MQ=255)
                                                                                                                                      ttttGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCt                >  3:241989/1‑149 (MQ=255)
                                                                                                                                        ttGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCt                <  5:327461/147‑1 (MQ=255)
                                                                                                                                           gggCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCg            <  8:25497/148‑1 (MQ=255)
                                                                                                                                           gggCACGGAAATCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCgg           >  6:96418/1‑149 (MQ=255)
                                                                                                                                             gCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCggg          >  5:48181/1‑148 (MQ=255)
                                                                                                                                             gCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCggg          >  1:28698/1‑148 (MQ=255)
                                                                                                                                                    tttCCGTGCCCATTTCACAAGGTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGGCGTTACCCTTTCCTGCCGGGAAAGTGAc  <  4:295729/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGAC  >  NC_000913/169600‑169896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: