Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 974,964 C→T 7.7% R216C (CGT→TGT)  mukF → chromosome condensin MukBEF, kleisin‑like subunit, binds calcium

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913974,9640CT 7.7% 238.5 / 7.4 79R216C (CGT→TGT) mukFchromosome condensin MukBEF, kleisin‑like subunit, binds calcium
Reads supporting (aligned to +/- strand):  ref base C (33/39);  new base T (3/3);  total (36/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.79e-01

TTTTCGACAGTATCGACCTGACGCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTAT  >  NC_000913/974818‑975109
                                                                                                                                                  |                                                                                                                                                 
ttttCGACAGTATCGACCTGACGCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGt                                                                                                                                                 <  2:217847/149‑1 (MQ=255)
    cGACAGTATCGACCTGACGCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGAATTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGaa                                                                                                                                              <  1:328847/148‑1 (MQ=255)
     gACAGTATCGACCTGACGCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAAtt                                                                                                                                            >  6:149840/1‑149 (MQ=255)
     gACAGTATCGACCTGACGCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGGTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGCACGCTGCGTGAAtt                                                                                                                                            >  1:112145/1‑149 (MQ=255)
        aGTATCGACCTGACGCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCa                                                                                                                                         <  1:126049/149‑1 (MQ=255)
        aGTATCGACCTGACGCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCa                                                                                                                                         <  3:206921/149‑1 (MQ=255)
            tCGACCTGACGCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGa                                                                                                                                      <  1:300269/148‑1 (MQ=255)
            tCGACCTGACGCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCATGAATTGCAGGa                                                                                                                                      <  8:122240/148‑1 (MQ=255)
                     cGCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGaa                                                                                                                            <  3:111128/149‑1 (MQ=255)
                      gCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAATGACGATTTCGACCAGTTGCTGACCAAAGAGTGGCGGGCGGCGAGTACCATCTGTGAATTGTTGCTTTCGGAAACTTCGGGAACGCTGCGTGAAGTGCAGGATTCGCGGGAAg                                                                                                                           >  6:260508/1‑149 (MQ=255)
                      gCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAg                                                                                                                           >  6:328302/1‑149 (MQ=255)
                      gCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAg                                                                                                                           >  7:198072/1‑149 (MQ=255)
                        aaCGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCg                                                                                                                         >  3:43589/1‑149 (MQ=255)
                                aTGGACGAACAGCAGCAGCAGGGGTAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGa                                                                                                                 <  1:112362/149‑1 (MQ=255)
                                    aCGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACaaa                                                                                                             >  3:320714/1‑149 (MQ=255)
                                    aCGAACAGCAGCAGCAGCTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGCGCGGCGATTTCCAGCTGTGAATTGTAGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGATGGAAGCGGCAGGCGACaaa                                                                                                             >  5:30188/1‑149 (MQ=255)
                                       aaCAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACGGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTg                                                                                                          <  8:258523/149‑1 (MQ=255)
                                         ccgcagcagcagGCGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCa                                                                                                        <  1:182385/147‑1 (MQ=255)
                                         cagcagcagcagGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCa                                                                                                        <  4:118941/149‑1 (MQ=255)
                                         cagcagcagcagGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCa                                                                                                        <  2:55163/149‑1 (MQ=255)
                                              gcagcagGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGTGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCt                                                                                                    >  1:283833/1‑148 (MQ=255)
                                                 gcagGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATc                                                                                                <  8:175452/149‑1 (MQ=255)
                                                    ggTGAAGGACGATATCGCCCAGTTGATGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGt                                                                                             >  7:251130/1‑149 (MQ=255)
                                                     gTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGTGTGAATTGCAGGATACGCTGGaa                                                                                                                            <  4:51783/117‑1 (MQ=255)
                                                     gTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGTGTGAATTGCAGGATACGCTGGaa                                                                                                                            >  3:51783/1‑117 (MQ=255)
                                                      tGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTg                                                                                           >  7:280489/1‑149 (MQ=255)
                                                           gACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTACAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTCCAGGATACGCTGGAAGCGTCAGGCGACAAATTGCAGCATAATCTGTTGCGCAt                                                                                      >  6:45893/1‑149 (MQ=255)
                                                            aCGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCAtt                                                                                     <  5:45893/149‑1 (MQ=255)
                                                             cGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTc                                                                                    <  4:204345/149‑1 (MQ=255)
                                                              gATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCAtt                                                                                     >  8:132835/1‑147 (MQ=255)
                                                                  tCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGAt                                                                               >  6:329240/1‑149 (MQ=255)
                                                                  tCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGAt                                                                               >  4:13810/1‑149 (MQ=255)
                                                                      ccAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGcga                                                                           <  7:16721/149‑1 (MQ=255)
                                                                      ccAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGcga                                                                           >  6:258554/1‑149 (MQ=255)
                                                                      ccAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGcga                                                                           >  2:158098/1‑149 (MQ=255)
                                                                       cAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCa                                                                                                        <  7:220736/119‑1 (MQ=255)
                                                                       cAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCa                                                                                                        >  8:220736/1‑119 (MQ=255)
                                                                       cAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGcgac                                                                          >  8:269346/1‑149 (MQ=255)
                                                                         gTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGcgacga                                                                        >  5:61064/1‑149 (MQ=255)
                                                                          ttGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGAt                                                                       >  4:315143/1‑149 (MQ=255)
                                                                            gCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTgc                                                                                          >  7:329415/1‑128 (MQ=255)
                                                                            gCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTgc                                                                                          <  8:329415/128‑1 (MQ=255)
                                                                             cTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGAc                                                                    >  2:194994/1‑149 (MQ=255)
                                                                              tGAACAAAGCCTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGCCGATGAcc                                                                   >  4:285721/1‑149 (MQ=255)
                                                                              tGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGTGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGAt                                                                       >  8:191045/1‑145 (MQ=255)
                                                                              tGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGTGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGAt                                                                       <  7:191045/145‑1 (MQ=255)
                                                                              tGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGAcc                                                                   >  2:171473/1‑149 (MQ=255)
                                                                                tatcaaGTCTGGCGGGCGGAGATTTCAAGCTGTGAATTCTTTCTTTCGGAAACATCCTGACAGCTGCGTGAATAGGATGATAGGCTGGAAGCGGCAGGCGACATATTGCATGCTTATCTGTTGTGCAGTCAGGATGAGACGATGAca                                                                   <  6:283671/143‑2 (MQ=255)
                                                                                  cAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATTCGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGt                                                                                             <  2:323043/119‑1 (MQ=255)
                                                                                   aaaGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGa                                                              <  5:258554/149‑1 (MQ=255)
                                                                                    aaGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGAc                                                             <  5:149840/149‑1 (MQ=255)
                                                                                     agccTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACg                                                            >  2:356452/4‑149 (MQ=255)
                                                                                      taCTGGCGGGCGGCGTTTTGCAGCTGTGAATTGGTGGTTTCGGAAACTTCCGGAACGATGGGTGATTTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTTCATGATAATCTGTTGCGCATTCTGGATGCGACGATGTCCCATGACGa                                                           <  6:30188/148‑1 (MQ=255)
                                                                                       aCTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGAt                                                          <  1:274113/149‑1 (MQ=255)
                                                                                        cTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATc                                                         <  1:158098/149‑1 (MQ=255)
                                                                                            cgggcggCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCa                                                     <  4:43589/149‑1 (MQ=255)
                                                                                              ggcggcGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCAtt                                                   <  2:46307/149‑1 (MQ=255)
                                                                                                cggcgATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTtc                                                 >  1:179759/1‑149 (MQ=255)
                                                                                                    gATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTTCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTtcgtc                                              >  1:266533/1‑148 (MQ=255)
                                                                                                        tCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGACCGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGCCGATCTGCATTTCGTCGATc                                          <  8:198072/148‑1 (MQ=255)
                                                                                                        tCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTTCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATc                                          <  1:78411/148‑1 (MQ=255)
                                                                                                        tCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATc                                          <  1:194994/148‑1 (MQ=255)
                                                                                                        tCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGAAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGCCGATCTGCATTTCGTCGATc                                          <  6:245856/148‑1 (MQ=255)
                                                                                                            gCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGTGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTg                                     <  2:283833/149‑1 (MQ=255)
                                                                                                            gCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTg                                     <  3:13810/149‑1 (MQ=255)
                                                                                                             cTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGAGAAATTGACCGCTAATCTGTTGAGCATTCAGGATGCGACGATGACCCATTACGAGCGGCATTTCGTCGAGCGTCTgt                                    >  6:70309/1‑148 (MQ=255)
                                                                                                                  aaTTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTtcgt                                               <  8:277808/133‑1 (MQ=255)
                                                                                                                  aaTTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTtcgt                                               >  7:277808/1‑133 (MQ=255)
                                                                                                                  aaTTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGtt                                <  5:329240/148‑1 (MQ=255)
                                                                                                                       ttgCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATc                           <  4:12965/148‑1 (MQ=255)
                                                                                                                       ttgCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCt                          >  4:152140/1‑149 (MQ=255)
                                                                                                                          cTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGc                        <  4:320714/148‑1 (MQ=255)
                                                                                                                          cTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCa                       <  8:280489/149‑1 (MQ=255)
                                                                                                                           tttCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTg                         >  1:234620/1‑146 (MQ=255)
                                                                                                                                     ttCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTc             >  7:44565/1‑148 (MQ=255)
                                                                                                                                     ttCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTc             >  6:128082/1‑148 (MQ=255)
                                                                                                                                        cGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATc         <  7:269346/149‑1 (MQ=255)
                                                                                                                                        cGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATc         <  3:315143/149‑1 (MQ=255)
                                                                                                                                               cTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGtattat  <  1:109519/149‑1 (MQ=255)
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TTTTCGACAGTATCGACCTGACGCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTAT  >  NC_000913/974818‑975109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: