Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,898,316 T→C 6.8% intergenic (+20/‑111) sdaA → / → yoaD L‑serine dehydratase 1/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase, regulator of cellulose production

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,898,3160TC 6.8% 220.4 / 5.3 73intergenic (+20/‑111)sdaA/yoaDL‑serine dehydratase 1/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase, regulator of cellulose production
Reads supporting (aligned to +/- strand):  ref base T (35/33);  new base C (3/2);  total (38/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

CGCACCAGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTAAAACCTATC  >  NC_000913/1898168‑1898460
                                                                                                                                                    |                                                                                                                                                
cgcACCAGTGCACCGCGCGTCTCCCTGCATAAGGTCATCGAAACGATGAACGAAACCGGTAAGCACATGCACGCCAAATACCGCGAAACCACACGCGGAGGTCTCGCAATCAAAGTCCAGTGTCACAAAAACTTCTTACTCGCACATCt                                                                                                                                                  >  2:129045/1‑149 (MQ=255)
           aCCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATgg                                                                                                                                       <  2:229783/149‑1 (MQ=255)
           aCCGCGCGTCTCGCTGGATAAAGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATgg                                                                                                                                       <  6:167413/149‑1 (MQ=255)
             cgcgcgTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGc                                                                                                                                     >  6:200482/1‑149 (MQ=255)
               cgcgTCTCGCTGGATAAGGTCATCGAAACGCTGTACGACACCGGTAAGGACAGGAACGCCAAATCCCGCGACACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCCGTTGCAGATGGGCGa                                                                                                                                   <  1:52893/149‑1 (MQ=255)
                   tctcGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGACCGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGAAACGCATGGGCGAAtt                                                                                                                                >  2:199595/1‑148 (MQ=255)
                   tctcGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAAttt                                                                                                                               <  2:114221/149‑1 (MQ=255)
                           gATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGc                                                                                                                       >  4:16720/1‑149 (MQ=255)
                              aaGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCttt                                                                                                                    >  7:109761/1‑149 (MQ=255)
                                 gTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGAGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTtctc                                                                                                                 >  5:139749/1‑149 (MQ=255)
                                     tCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTct                                                                                                             <  4:218498/149‑1 (MQ=255)
                                     tCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTct                                                                                                             <  6:49382/149‑1 (MQ=255)
                                     tCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTct                                                                                                             >  8:281261/1‑149 (MQ=255)
                                     tCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACAGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTct                                                                                                             >  2:310445/1‑149 (MQ=255)
                                     tCGAAACGATCTACGAAACCGGTAAGGACATGAACGCCAAAAACCGAGAAACCACACGCGGTGGTCTGGATATCAAAGTCCAGTGTGCCTAATACTTCTAACTCGCCCATCTGCAACGCCTGCGCGAATTTATCCCCGCTTtatcctag                                                                                                             >  5:331369/1‑145 (MQ=255)
                                      cGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTctg                                                                                                            >  8:319386/1‑149 (MQ=255)
                                              tGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGTATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAata                                                                                                    >  8:121167/1‑149 (MQ=255)
                                              tGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAata                                                                                                    <  6:7695/149‑1 (MQ=255)
                                              tGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAata                                                                                                    <  5:116215/149‑1 (MQ=255)
                                                tACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATAtt                                                                                                  <  8:295461/149‑1 (MQ=255)
                                                tACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATAtt                                                                                                  >  1:237055/1‑149 (MQ=255)
                                                 aCGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTc                                                                                                 >  8:190040/1‑149 (MQ=255)
                                                 aCGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTc                                                                                                 >  3:94201/1‑149 (MQ=255)
                                                  cGAACCCGGTAAGGACATGCACGCCAAATGCCGCGAAACCTCACGCGGTGGGCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGCTGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTcc                                                                                                <  5:37765/149‑1 (MQ=255)
                                                  cGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTcc                                                                                                <  8:8142/149‑1 (MQ=255)
                                                   gAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTccc                                                                                               <  8:109761/149‑1 (MQ=255)
                                                   gAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTccc                                                                                               >  2:179287/1‑149 (MQ=255)
                                                     aaCCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGAGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCa                                                                                             >  8:138953/1‑149 (MQ=255)
                                                          gTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTaca                                                                                        <  6:139749/149‑1 (MQ=255)
                                                          gTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTac                                                                                         >  6:274409/1‑148 (MQ=255)
                                                               gACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTc                                                                                    >  7:119480/1‑148 (MQ=255)
                                                                     aaCGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGtt                                                                             <  4:94201/149‑1 (MQ=255)
                                                                           aaaTACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCa                                                                       <  1:229969/149‑1 (MQ=255)
                                                                                  gcgAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTcc                                                                                   <  2:342144/130‑1 (MQ=255)
                                                                                  gcgAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGtt                                                                             <  5:247378/136‑1 (MQ=255)
                                                                                  gcgAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGtt                                                                             >  6:247378/1‑136 (MQ=255)
                                                                                    gAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGt                                                              <  3:16720/149‑1 (MQ=255)
                                                                                        ccTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGc                                                           >  3:10638/1‑148 (MQ=255)
                                                                                         cTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCa                                                         >  7:96938/1‑149 (MQ=255)
                                                                                         cTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCa                                                         <  2:237055/149‑1 (MQ=255)
                                                                                         cTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGAGGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCa                                                         <  6:86740/149‑1 (MQ=255)
                                                                                          tCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCa                                                         >  2:285161/1‑148 (MQ=255)
                                                                                            aCGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCAt                                                        <  1:179287/147‑1 (MQ=255)
                                                                                            aCGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCAt                                                        <  7:319386/147‑1 (MQ=255)
                                                                                                     tCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCTAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTTCGTCAGGCGTTTGTCGCCATACGCTTACAgg                                             <  3:110683/149‑1 (MQ=255)
                                                                                                     tCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTGGTCGCCATACGCTTACAgg                                             <  1:129045/149‑1 (MQ=255)
                                                                                                       tGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCCGTTGCAGATGGGCGag                                                                                                                                  >  2:252159/1‑61 (MQ=21)
                                                                                                       tGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCCGTTGCAGATGGGCGag                                                                                                                                  <  1:252159/62‑2 (MQ=21)
                                                                                                         gCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTgg                                         >  2:14765/1‑149 (MQ=255)
                                                                                                         gCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTgg                                         >  1:315229/1‑149 (MQ=255)
                                                                                                                aaGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGc                                    <  4:180907/147‑1 (MQ=255)
                                                                                                                aaGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGc                                    >  3:180907/1‑147 (MQ=255)
                                                                                                                aaGTCCAGTGTGACTAATACTTCTTACTCGCCCAGCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCAt                                  <  5:200482/149‑1 (MQ=255)
                                                                                                                   tCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCa                               <  4:10638/149‑1 (MQ=255)
                                                                                                                       gtgtGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCaaaaa                           <  5:81631/149‑1 (MQ=255)
                                                                                                                        tgtgACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAg                          >  5:126191/1‑149 (MQ=255)
                                                                                                                        tgtgACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAg                          <  1:310445/149‑1 (MQ=255)
                                                                                                                             cTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACaa                     >  1:49094/1‑149 (MQ=255)
                                                                                                                             cTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACaa                     >  5:91674/1‑149 (MQ=255)
                                                                                                                                aTACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACg                   >  5:177640/1‑148 (MQ=255)
                                                                                                                                 tACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCGCCACTACACTTCCACTGTTGCGTCAGGCGTATGTCGCCATACGCTTACCGGGTGGCCCGCATGCAAAAAGCACAACg                   >  2:122687/1‑147 (MQ=255)
                                                                                                                                   cttcttACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATc               <  7:190040/149‑1 (MQ=255)
                                                                                                                                    ttcttACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTAGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCa              >  6:289777/1‑149 (MQ=255)
                                                                                                                                       ttACGCCCCCATCTGCAACGGAAGGGCGAATTTATACCCGCTTTGACGTCTGCTGTTATATTCCCCACTACACTTCCAATTTTGGGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCAtt            <  5:274409/148‑1 (MQ=255)
                                                                                                                                        tACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTa           >  8:318286/1‑148 (MQ=255)
                                                                                                                                         aCTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTa           <  7:281261/147‑1 (MQ=255)
                                                                                                                                          ttCGCCCATCCGTTGCAGATGGGCGAATTTATTCCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTaaaa        >  1:13635/2‑149 (MQ=255)
                                                                                                                                          ttCGCCCATCCGTTGCAGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACGACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTaaaa        >  6:110655/2‑149 (MQ=255)
                                                                                                                                           tCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTAAAAc       >  7:91406/1‑149 (MQ=255)
                                                                                                                                           tCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTAAAAc       >  2:182775/1‑149 (MQ=255)
                                                                                                                                             gCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGTGTGGCCCGCATGCAAAAAGCACAACGGATCATTAAAAc       <  8:96938/147‑1 (MQ=255)
                                                                                                                                             gCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTAAAAcc      >  7:153680/1‑148 (MQ=255)
                                                                                                                                             gCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTAAAAcc      >  4:128252/1‑148 (MQ=255)
                                                                                                                                               ccATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAGCACAACGGATCATTAAAACCTAtc  <  8:119480/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CGCACCAGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTAAAACCTATC  >  NC_000913/1898168‑1898460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: