Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,898,319 A→T 6.9% intergenic (+23/‑108) sdaA → / → yoaD L‑serine dehydratase 1/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase, regulator of cellulose production

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,898,3190AT 6.9% 215.8 / 5.4 73intergenic (+23/‑108)sdaA/yoaDL‑serine dehydratase 1/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase, regulator of cellulose production
Reads supporting (aligned to +/- strand):  ref base A (35/33);  new base T (3/2);  total (38/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.11e-01

ACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTAAAACCTATC  >  NC_000913/1898179‑1898460
                                                                                                                                            |                                                                                                                                             
aCCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATgg                                                                                                                                       <  2:229783/149‑1 (MQ=255)
aCCGCGCGTCTCGCTGGATAAAGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATgg                                                                                                                                       <  6:167413/149‑1 (MQ=255)
  cgcgcgTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGc                                                                                                                                     >  6:200482/1‑149 (MQ=255)
    cgcgTCTCGCTGGATAAGGTCATCGAAACGCTGTACGACACCGGTAAGGACAGGAACGCCAAATCCCGCGACACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCCGTTGCAGATGGGCGa                                                                                                                                   <  1:52893/149‑1 (MQ=255)
        tctcGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGACCGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGAAACGCATGGGCGAAtt                                                                                                                                >  2:199595/1‑148 (MQ=255)
        tctcGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAAttt                                                                                                                               <  2:114221/149‑1 (MQ=255)
                gATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGc                                                                                                                       >  4:16720/1‑149 (MQ=255)
                   aaGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCttt                                                                                                                    >  7:109761/1‑149 (MQ=255)
                      gTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGAGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTtctc                                                                                                                 >  5:139749/1‑149 (MQ=255)
                          tCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTct                                                                                                             >  8:281261/1‑149 (MQ=255)
                          tCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTct                                                                                                             <  4:218498/149‑1 (MQ=255)
                          tCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTct                                                                                                             <  6:49382/149‑1 (MQ=255)
                          tCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACAGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTct                                                                                                             >  2:310445/1‑149 (MQ=255)
                          tCGAAACGATCTACGAAACCGGTAAGGACATGAACGCCAAAAACCGAGAAACCACACGCGGTGGTCTGGATATCAAAGTCCAGTGTGCCTAATACTTCTAACTCGCCCATCTGCAACGCCTGCGCGAATTTATCCCCGCTTtatcctag                                                                                                             >  5:331369/1‑145 (MQ=255)
                           cGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTctg                                                                                                            >  8:319386/1‑149 (MQ=255)
                                   tGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGTATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAata                                                                                                    >  8:121167/1‑149 (MQ=255)
                                   tGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAata                                                                                                    <  6:7695/149‑1 (MQ=255)
                                   tGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAata                                                                                                    <  5:116215/149‑1 (MQ=255)
                                     tACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATAtt                                                                                                  <  8:295461/149‑1 (MQ=255)
                                     tACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATAtt                                                                                                  >  1:237055/1‑149 (MQ=255)
                                      aCGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTc                                                                                                 >  3:94201/1‑149 (MQ=255)
                                      aCGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTc                                                                                                 >  8:190040/1‑149 (MQ=255)
                                       cGAACCCGGTAAGGACATGCACGCCAAATGCCGCGAAACCTCACGCGGTGGGCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGCTGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTcc                                                                                                <  5:37765/149‑1 (MQ=255)
                                       cGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTcc                                                                                                <  8:8142/149‑1 (MQ=255)
                                        gAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTccc                                                                                               >  2:179287/1‑149 (MQ=255)
                                        gAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTccc                                                                                               <  8:109761/149‑1 (MQ=255)
                                          aaCCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGAGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCa                                                                                             >  8:138953/1‑149 (MQ=255)
                                               gTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTaca                                                                                        <  6:139749/149‑1 (MQ=255)
                                               gTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTac                                                                                         >  6:274409/1‑148 (MQ=255)
                                                    gACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTc                                                                                    >  7:119480/1‑148 (MQ=255)
                                                          aaCGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGtt                                                                             <  4:94201/149‑1 (MQ=255)
                                                                aaaTACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCa                                                                       <  1:229969/149‑1 (MQ=255)
                                                                       gcgAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTcc                                                                                   <  2:342144/130‑1 (MQ=255)
                                                                       gcgAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGtt                                                                             <  5:247378/136‑1 (MQ=255)
                                                                       gcgAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGtt                                                                             >  6:247378/1‑136 (MQ=255)
                                                                         gAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGt                                                              <  3:16720/149‑1 (MQ=255)
                                                                             ccTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGc                                                           >  3:10638/1‑148 (MQ=255)
                                                                              cTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCa                                                         >  7:96938/1‑149 (MQ=255)
                                                                              cTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCa                                                         <  2:237055/149‑1 (MQ=255)
                                                                              cTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGAGGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCa                                                         <  6:86740/149‑1 (MQ=255)
                                                                               tCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCa                                                         >  2:285161/1‑148 (MQ=255)
                                                                                 aCGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCAt                                                        <  7:319386/147‑1 (MQ=255)
                                                                                 aCGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCAt                                                        <  1:179287/147‑1 (MQ=255)
                                                                                          tCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCTAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTTCGTCAGGCGTTTGTCGCCATACGCTTACAgg                                             <  3:110683/149‑1 (MQ=255)
                                                                                          tCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTGGTCGCCATACGCTTACAgg                                             <  1:129045/149‑1 (MQ=255)
                                                                                            tGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCCGTTGCAGATGGGCGag                                                                                                                                  <  1:252159/62‑2 (MQ=21)
                                                                                            tGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCCGTTGCAGATGGGCGag                                                                                                                                  >  2:252159/1‑61 (MQ=21)
                                                                                              gCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTgg                                         >  1:315229/1‑149 (MQ=255)
                                                                                              gCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTgg                                         >  2:14765/1‑149 (MQ=255)
                                                                                                     aaGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGc                                    >  3:180907/1‑147 (MQ=255)
                                                                                                     aaGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGc                                    <  4:180907/147‑1 (MQ=255)
                                                                                                     aaGTCCAGTGTGACTAATACTTCTTACTCGCCCAGCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCAt                                  <  5:200482/149‑1 (MQ=255)
                                                                                                        tCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCa                               <  4:10638/149‑1 (MQ=255)
                                                                                                            gtgtGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCaaaaa                           <  5:81631/149‑1 (MQ=255)
                                                                                                             tgtgACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAg                          <  1:310445/149‑1 (MQ=255)
                                                                                                             tgtgACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAg                          >  5:126191/1‑149 (MQ=255)
                                                                                                                  cTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACaa                     >  1:49094/1‑149 (MQ=255)
                                                                                                                  cTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACaa                     >  5:91674/1‑149 (MQ=255)
                                                                                                                     aTACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACg                   >  5:177640/1‑148 (MQ=255)
                                                                                                                      tACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCGCCACTACACTTCCACTGTTGCGTCAGGCGTATGTCGCCATACGCTTACCGGGTGGCCCGCATGCAAAAAGCACAACg                   >  2:122687/1‑147 (MQ=255)
                                                                                                                        cttcttACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATc               <  7:190040/149‑1 (MQ=255)
                                                                                                                         ttcttACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTAGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCa              >  6:289777/1‑149 (MQ=255)
                                                                                                                            ttACGCCCCCATCTGCAACGGAAGGGCGAATTTATACCCGCTTTGACGTCTGCTGTTATATTCCCCACTACACTTCCAATTTTGGGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCAtt            <  5:274409/148‑1 (MQ=255)
                                                                                                                             tACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTa           >  8:318286/1‑148 (MQ=255)
                                                                                                                              aCTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTa           <  7:281261/147‑1 (MQ=255)
                                                                                                                               ttCGCCCATCCGTTGCAGATGGGCGAATTTATTCCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTaaaa        >  1:13635/2‑149 (MQ=255)
                                                                                                                               ttCGCCCATCCGTTGCAGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACGACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTaaaa        >  6:110655/2‑149 (MQ=255)
                                                                                                                                tCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTAAAAc       >  7:91406/1‑149 (MQ=255)
                                                                                                                                tCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTAAAAc       >  2:182775/1‑149 (MQ=255)
                                                                                                                                  gCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGTGTGGCCCGCATGCAAAAAGCACAACGGATCATTAAAAc       <  8:96938/147‑1 (MQ=255)
                                                                                                                                  gCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTAAAAcc      >  7:153680/1‑148 (MQ=255)
                                                                                                                                  gCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTAAAAcc      >  4:128252/1‑148 (MQ=255)
                                                                                                                                    ccATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAGCACAACGGATCATTAAAACCTAtc  <  8:119480/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                             
ACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTAAAACCTATC  >  NC_000913/1898179‑1898460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: