Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,806,319	C→G	intergenic (‑236/‑51)	ydiY ← / → pfkB	acid‑inducible putative outer membrane protein/6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,806,319	0	C	G	100.0%	279.4 / NA	77	intergenic (‑236/‑51)	ydiY/pfkB	acid‑inducible putative outer membrane protein/6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (37/40);  total (37/40)

GAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCG  >  NC_000913/1806174‑1806463                                                                                                                                                    |                                                                                                                                                 gAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAATGCTCCAATAAATCATATTGTTAATTTCTTGACt                                                                                                                                               >  5:393779/1‑149 (MQ=255) gAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACt                                                                                                                                               >  8:35587/1‑149 (MQ=255) gAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACt                                                                                                                                               >  8:291762/1‑149 (MQ=255) gAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACt                                                                                                                                               >  4:312606/1‑149 (MQ=255)   aTTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACttt                                                                                                                                             >  1:427265/1‑149 (MQ=255)    ttATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCTAAAACAGATTTTTATT‑‑TATATATATTTATATGCATGATTTTAAATAAAGCTCCAATAAATCATATTGTTAATATCTAGACttt                                                                                                                                             >  2:38809/1‑148 (MQ=255)    ttATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTc                                                                                                                                            <  4:372936/149‑1 (MQ=255)       taAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAATGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCg                                                                                                                                          <  6:393779/148‑1 (MQ=255)        aaaaaGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCt                                                                                                                                        >  5:143936/1‑149 (MQ=255)        aaaaaGGCGCGAAGAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCt                                                                                                                                        >  4:162947/1‑149 (MQ=255)          aaaGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGa                                                                                                                                      <  3:312606/149‑1 (MQ=255)              gcgcGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCg                                                                                                                                  <  6:155293/149‑1 (MQ=255)              gcgcGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGAGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCg                                                                                                                                  <  4:82892/149‑1 (MQ=255)                gcgAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGt                                                                                                                                <  6:143936/149‑1 (MQ=255)                 ctaaTAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGTCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTg                                                                                                                               <  2:302826/147‑1 (MQ=255)                  gAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAATCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAGATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGc                                                                                                                              <  1:38809/149‑1 (MQ=255)                  gAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGc                                                                                                                              >  7:200789/1‑149 (MQ=255)                      aaCTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGa                                                                                                                          >  4:207753/1‑149 (MQ=255)                       aCTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGa                                                                                                                          <  7:412149/148‑1 (MQ=255)                       aCTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGAc                                                                                                                         <  8:200789/149‑1 (MQ=255)                        cTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACt                                                                                                                        >  2:406497/1‑149 (MQ=255)                        cTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACt                                                                                                                        <  3:221548/149‑1 (MQ=255)                            gCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGaaa                                                                                                                    <  7:181660/149‑1 (MQ=255)                             cAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAAt                                                                                                                   >  5:155798/1‑149 (MQ=255)                              aaTGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGt                                                                                                                                >  2:374483/1‑135 (MQ=255)                               aTGTATTCTTCTTTCATTTTTTGAATACTCATGTGGCGAAAACAGAGTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCACTAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCa                                                                                                                 <  2:106896/149‑1 (MQ=255)                               aTGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCa                                                                                                                 <  7:291762/149‑1 (MQ=255)                               aTGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCa                                                                                                                 <  7:35587/149‑1 (MQ=255)                               aTGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCa                                                                                                                 <  4:37777/149‑1 (MQ=255)                                  tATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGc                                                                                                               <  2:294087/148‑1 (MQ=255)                                    ttCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCta                                                                                                            <  5:335463/149‑1 (MQ=255)                                         aTTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATag                                                                                                         <  2:427265/147‑1 (MQ=255)                                          tttCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATaggag                                                                                                      >  4:362418/1‑149 (MQ=255)                                           ttCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATaggag                                                                                                      >  3:74787/1‑148 (MQ=255)                                               ttttttGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAat                                                                                                 >  5:80752/1‑149 (MQ=255)                                                  tttGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAatgat                                                                                              >  8:140371/1‑149 (MQ=255)                                                  tttGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAatga                                                                                               <  8:412234/148‑1 (MQ=255)                                                   ttGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAatgat                                                                                              <  6:155798/148‑1 (MQ=255)                                                    tGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAatgatg                                                                                             <  1:11949/148‑1 (MQ=255)                                                     gAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAatgatg                                                                                             >  6:190409/1‑149 (MQ=255)                                                     gAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATgg                                                                                            >  1:432637/1‑148 (MQ=255)                                                       aTAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTAc                                                                                         <  8:391608/149‑1 (MQ=255)                                                       aTAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTAc                                                                                         <  1:47158/149‑1 (MQ=255)                                                       aTAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTAc                                                                                         >  2:47158/1‑149 (MQ=255)                                                             aTGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATAATGTTAATTTCTTGACTTTCAGCTGATTCGATGCAAGAATGAAATCATCCTATAGGAGGAAATGATGGTACGTATc                                                                                    >  1:412529/1‑148 (MQ=255)                                                              tgtgGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCta                                                                                  <  1:54888/149‑1 (MQ=255)                                                                tgGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCtat                                                                                 <  7:393546/148‑1 (MQ=255)                                                                  gCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATAc                                                                               <  3:162947/148‑1 (MQ=255)                                                                    gAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAatgat                                                                                              >  1:396706/1‑131 (MQ=255)                                                                    gAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAatgat                                                                                              <  2:396706/131‑1 (MQ=255)                                                                    gAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGtt                                                                            <  3:362418/149‑1 (MQ=255)                                                                              ttttATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTgcg                                                                  >  4:113742/1‑149 (MQ=255)                                                                                 tatt‑‑tatATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGcc                                                                <  4:74787/148‑1 (MQ=255)                                                                                        atatatatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCctctct                                                          >  5:324872/1‑149 (MQ=255)                                                                                           tatatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTTATTTCTTGACTTTCCGCTGATTCGGTGCCAGCCTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCg                                                        <  2:64058/148‑1 (MQ=255)                                                                                            atatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGa                                                       >  1:323396/1‑148 (MQ=255)                                                                                            atatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGa                                                       >  1:162863/1‑148 (MQ=255)                                                                                             tatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACTTATCTATACGTTGACACTTGCGCCCTCTCTCGa                                                       >  3:87944/1‑147 (MQ=255)                                                                                             tatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATa                                                     <  7:140371/149‑1 (MQ=255)                                                                                                        aaaaTTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAAtt                                          <  1:406497/149‑1 (MQ=255)                                                                                                            ttttAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTAccc                                      >  5:380020/1‑149 (MQ=255)                                                                                                                    aaaGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACtt                                                                     <  4:146794/110‑1 (MQ=255)                                                                                                                    aaaGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACtt                                                                     >  3:146794/1‑110 (MQ=255)                                                                                                                     aaGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAAttt                             <  6:324872/149‑1 (MQ=255)                                                                                                                         tCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTAcc                                       >  4:336426/1‑135 (MQ=255)                                                                                                                           cAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATccc                        >  5:325460/1‑148 (MQ=255)                                                                                                                            aaTAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCg                       >  4:190014/1‑148 (MQ=255)                                                                                                                             aTAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGaa                     <  2:412529/149‑1 (MQ=255)                                                                                                                                 aTCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAAt                               >  7:188948/1‑135 (MQ=255)                                                                                                                                 aTCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAAt                               <  8:188948/135‑1 (MQ=255)                                                                                                                                     tatTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACt             <  6:380020/149‑1 (MQ=255)                                                                                                                                       ttGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTgc           >  4:275352/1‑149 (MQ=255)                                                                                                                                          ttAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATTGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTAcc                                       <  5:142333/118‑1 (MQ=255)                                                                                                                                          ttAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTAcc                                       >  6:142333/1‑118 (MQ=255)                                                                                                                                            aaTTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCctc                                                             <  4:306985/94‑1 (MQ=255)                                                                                                                                            aaTTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCctc                                                             >  3:306985/1‑94 (MQ=255)                                                                                                                                            aaTTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTg       >  1:192017/1‑148 (MQ=255)                                                                                                                                                tcttgACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCg  >  8:274249/1‑149 (MQ=255)                                                                                                                                                 cttgACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCg  >  2:116965/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                                 GAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATT‑‑TATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCG  >  NC_000913/1806174‑1806463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑