New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3595984 =35 (1.260)4 (0.150) 4/252 3.0 12.5% coding (420/927 nt) livH branched‑chain amino acid ABC transporter permease
?NC_000913 3596035 = 23 (0.870)coding (369/927 nt) livH branched‑chain amino acid ABC transporter permease
Rejected: Frequency below/above cutoff threshold.

CTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACATACCGATTGCAGAGATGAGTGCAATCAGGCGCTTAGAGTTACGCACCGGGCGGTAAGCCAC  >  NC_000913/3595836‑3596124
                                                                                                                                                    |                                                                                                                                            
tgcgggattcgtcggcgcaatcgtcattgccagcgcctacggctggagtatcgaacgggtggcttaccgcccggtgcgtaactctaagcgcctgattgcactcatctctgcaatcggtatgtccatcttcctgcaaaactacgtcagcc                                                                                                                                              <  3:113413‑M1/1‑1 (MQ=255)
     gcgcAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGAcc                                                                                                                                         >  6:118762/1‑149 (MQ=255)
       gcAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCg                                                                                                                                        >  7:79996/1‑148 (MQ=255)
       gcAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCg                                                                                                                                        <  8:79996/148‑1 (MQ=255)
        cAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGtt                                                                                                                                      <  4:5502/149‑1 (MQ=255)
           gcccGACACGCGCGACCCCTGCGGGAATAGCGAATGACACTCGTCCGCGCCAGCATGGCGCGGAAGGTAACACTCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTaaa                                                                                                                                   <  8:95685/146‑1 (MQ=255)
           gCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTaaa                                                                                                                                   <  5:118762/149‑1 (MQ=255)
             aCGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTaaa                                                                                                                                   <  8:95686/147‑1 (MQ=255)
             aCGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTaaa                                                                                                                                   >  7:95686/1‑147 (MQ=255)
                        gACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAgc                                                                                                                      <  4:87907/149‑1 (MQ=255)
                              tGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACg                                                                                                                >  6:17924/1‑149 (MQ=255)
                                        gCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGaa                                                                                                       <  5:17924/148‑1 (MQ=255)
                                                     gTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACa                                                                                                                                 <  2:31331/109‑1 (MQ=255)
                                                     gTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACa                                                                                                                                 >  1:31331/1‑109 (MQ=255)
                                                          cgcCAGCATAGCGAGTAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTATACGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACg                                                                                     >  8:36402/1‑148 (MQ=255)
                                                           tggcttaccgcccggtgcgtaactctaagcgcctgattgcactcatctctgcaatcggtatgtccatcttcctgcaaaactacgtcagcCt                                                                                                                                             >  7:62434‑M1/90‑91 (MQ=255)
                                                           tggcttaccgcccggtgcgtaactctaagcgcctgattgcactcatctctgcaatcggtatgtccatcttcctgcaaaactacgtcagcCt                                                                                                                                             <  8:62434‑M1/2‑1 (MQ=255)
                                                             cAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGt                                                                                 >  3:33629/1‑149 (MQ=255)
                                                                 accgcccggtgcttaactctaagctcctgattgcactcatctctgcaatcggtatgtccatcttcctgcaaaactacgtcagcCTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAg                                                                                            >  8:94291‑M1/84‑134 (MQ=255)
                                                                 accgcccggtgcgtaactctaagctcctgattgcactcatctctgcaatcggtatgtccatcttcctgcaaaactacgtcagcCTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAg                                                                                            <  7:94291‑M1/51‑1 (MQ=255)
                                                                         gAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTcgc                                                                                                            >  5:80647/1‑110 (MQ=255)
                                                                         gAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTcgc                                                                                                            <  6:80647/110‑1 (MQ=255)
                                                                               aaCAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCa                                                                           <  2:111006/137‑1 (MQ=255)
                                                                               aaCAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACAGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCa                                                                           >  1:111006/1‑137 (MQ=255)
                                                                                  tctaagcgcctgattgcactcatctctgcaatcggtatgtccatcttcctgcaaaactacgtcagcCTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAAc                                                                                                      <  5:43108‑M1/41‑1 (MQ=255)
                                                                                  tctaagcgcctgattgcactcatctctgcaatcggtatgtccatcttcctgcaaaactacgtcagcCTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAAc                                                                                                      >  6:43108‑M1/67‑107 (MQ=255)
                                                                                  aaTCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACATAc                                                            <  4:33629/149‑1 (MQ=255)
                                                                                             ccGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACATACCGATTGCa                                                    >  1:1287/1‑146 (MQ=255)
                                                                                                                    gCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACATACCGATTGCAGAGATGAGTGCAATCAGGCGCTTaga                          <  2:26896/149‑1 (MQ=255)
                                                                                                                       gagaAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACATACCGATTGCAGAGATGAGTGCAATCAGGCGCTTAGAGtt                       <  7:36402/149‑1 (MQ=255)
                                                                                                                        agaAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGc                                                                                          <  7:13498/81‑1 (MQ=255)
                                                                                                                        agaAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGc                                                                                          >  8:13498/1‑81 (MQ=255)
                                                                                                                        agaAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACATACCGATTGCAGAGATGAGTGCAATCAGGCGCtt                             >  5:55458/1‑142 (MQ=255)
                                                                                                                        agaAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACATACCGATTGCAGAGATGAGTGCAATCAGGCGCtt                             <  6:55458/142‑1 (MQ=255)
                                                                                                                             ttttCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACATACCGATTGCAGAGATGAGTGCAATCAGGCGCTTAGAGTTACGCAc                 <  2:1287/149‑1 (MQ=255)
                                                                                                                                        ccccACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACATACCGATTGCAGAGATGAGTGCAATCAGGCGCTTAGAGTTACGCACCGGGCGGTAAg      >  4:113413/1‑149 (MQ=255)
                                                                                                                                         cccACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACATACCGATTGCAGAGATGAGTGCAATCAGGCGCTTAGAGTTACGCACCGGGCGGTAAGc     >  1:98880/1‑149 (MQ=255)
                                                                                                                                          ccaccaCCCACTGACCGCTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACATACCGATTGCAGAGATGAGTGCAATCAGGCGCTTAGAGTTACGCACCGGGCGGTAAGc     >  1:112384/1‑148 (MQ=255)
                                                                                                                                            agcacccacTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCTGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACATACCGATTGCAGAGATGAGTGCAATCAGgcgc                               <  6:5914/118‑1 (MQ=255)
                                                                                                                                            accaccCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGa                                                                 >  4:29166/1‑86 (MQ=255)
                                                                                                                                            accaccCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGa                                                                 <  3:29166/86‑1 (MQ=255)
                                                                                                                                            accaccCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACATACCGATTGCAGAGATGAGTGCAATCAGGCGCTTAGAGTTACGCACCGGGCGGTAAGCCAc  <  2:98880/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
CTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACATACCGATTGCAGAGATGAGTGCAATCAGGCGCTTAGAGTTACGCACCGGGCGGTAAGCCAC  >  NC_000913/3595836‑3596124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.