Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,806,340	C→T	intergenic (‑257/‑30)	ydiY ← / → pfkB	acid‑inducible putative outer membrane protein/6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,806,340	0	C	T	100.0%	66.3 / NA	20	intergenic (‑257/‑30)	ydiY/pfkB	acid‑inducible putative outer membrane protein/6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/10);  total (10/10)

TATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGG  >  NC_000913/1806207‑1806469                                                                                                                                      |                                                                                                                                  tATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGc                                                                                                                     <  1:135986/148‑1 (MQ=255)     cTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGa                                                                                                                                <  6:35991/133‑1 (MQ=255)     cTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGa                                                                                                                                >  5:35991/1‑133 (MQ=255)             aTTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGaaa                                                                                                        >  5:62805/1‑149 (MQ=255)                ttttGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATATGAAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGTTGCTAGACTGAAATCAGCATATAGGAGGAAatga                                                                                                     >  1:129185/1‑149 (MQ=255)                    gAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGTTGCTAGACTGAAATCAGCCTATAGGATGAAatgatgta                                                                                                 >  3:22101/1‑147 (MQ=255)                    gAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAatgatg                                                                                                   <  4:22101/147‑1 (MQ=255)                     aaTAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGt                                                                                                 <  3:106119/148‑1 (MQ=255)                             tgtgGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCta                                                                                        >  2:64007/1‑149 (MQ=255)                               tttcGAAAAAAGATTTTTATTTATATATATTTATCTGCAAAATTTTCAATAAAGCTCCAATAACTCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACTTATCtat                                                                                       <  5:79540/145‑1 (MQ=255)                               tgGCGAAAACATATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCAAATAAATCATATTGTTAATTTCTTCACTTTACGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCtat                                                                                       <  5:81294/148‑1 (MQ=255)                                 gCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACg                                                                                    <  6:62805/149‑1 (MQ=255)                                     aaaCAGATTTTTATTTATATATATTTATCTTCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGa                                                                                <  2:129185/149‑1 (MQ=255)                                          gATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACtt                                                                           <  1:64007/149‑1 (MQ=255)                                                                   gCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTACTTCACTTTCCGCTGATTAGGTGCTAGAGTGAAATCAGCGTATAGGAGGATATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCTcaacaa                                                  >  7:35270/1‑149 (MQ=255)                                                                                                  tatTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGATGGAAAACt                   >  4:90641/1‑149 (MQ=255)                                                                                                   atTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTAt                                 <  7:84507/134‑1 (MQ=255)                                                                                                   atTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTAt                                 >  8:84507/1‑134 (MQ=255)                                                                                                          aTTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAatga                                                                                                     >  7:81915/1‑59 (MQ=255)                                                                                                                   cTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCgg  >  6:70728/1‑149 (MQ=255)                                                                                                                                      |                                                                                                                                  TATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGG  >  NC_000913/1806207‑1806469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑