Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 3,560,930 IS5 (+) +4 bp coding (520‑523/831 nt) glpG ← rhomboid intramembrane serine protease

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3560930 =0 (0.000)47 (1.270) 39/254 0.1 100% coding (523/831 nt) glpG rhomboid intramembrane serine protease
?NC_000913 3652036 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 = 35609330 (0.000)38 (1.020) 35/254 0.2 100% coding (520/831 nt) glpG rhomboid intramembrane serine protease
?NC_000913 = 3653230 NA (NA)noncoding (1/1195 nt) IS5 repeat region

GAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTT  >  NC_000913/3653091‑3653378
                                                                                                                                           |                                                                                                                                                    
gAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGa                                                                                                                                              >  1:190291/1‑148 (MQ=31)
gAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGa                                                                                                                                              <  2:190291/148‑1 (MQ=31)
      ctctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTAtt                                                                                                                                       >  5:1258/1‑149 (MQ=35)
           ttCTGGTCTGACGTCGCTTTCTTCTTAATTCACTGTTGGCGAAGGTAAGTTGATGACTCATGATGAAGTCTGTTCTATGGCTCCAGATGTCAAACATGATCTCATATCAGTGACTTGTGCTCTCCTTCCTTAGGTGACACTAtttt                                                                                                                                     <  8:99493/146‑1 (MQ=11)
           ttCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTAtttttt                                                                                                                                   <  5:40454/148‑1 (MQ=39)
                  cTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATaaa                                                                                                                           <  6:1258/149‑1 (MQ=255)
                            ttACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACAt                                                                                                                              <  1:111396/136‑1 (MQ=255)
                            ttACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACAt                                                                                                                              >  2:111396/1‑136 (MQ=255)
                               ctgctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTAcc                                                                                                               <  7:3563/148‑1 (MQ=255)
                                       ttCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGGACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATta                                                                                                      >  3:41269/1‑149 (MQ=255)
                                                              gatgaCTCATGATGGACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTg                                                                                <  4:41269/148‑1 (MQ=255)
                                                                    tCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGacaaac                                                                          >  8:34029/1‑148 (MQ=255)
                                                                    tCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGacaaac                                                                          >  6:2585/1‑148 (MQ=255)
                                                                        gatgaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACAt                                                                                                                              >  4:39371/1‑92 (MQ=255)
                                                                        gatgaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACAt                                                                                                                              <  3:39371/92‑1 (MQ=255)
                                                                             aCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAgg                                                                >  7:121033/1‑149 (MQ=255)
                                                                              cccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCACCAAATTATATAAAGATGGACAATATCTTTACAAAAAAATCGAAGGt                                                               >  8:31111/1‑149 (MQ=255)
                                                                               ccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCtt                                                                                 >  1:180695/1‑130 (MQ=255)
                                                                               ccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCtt                                                                                 <  2:180695/130‑1 (MQ=255)
                                                                                        tGGATCCAGATTACAAACATTATCTCATATCAGGTACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCa                                                      <  7:31111/148‑1 (MQ=255)
                                                                                         ggCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCaa                                                     >  3:63594/1‑148 (MQ=255)
                                                                                                       aaCATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATg                                                                                            >  6:123332/1‑95 (MQ=255)
                                                                                                       aaCATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATg                                                                                            <  5:123332/95‑1 (MQ=255)
                                                                                                         cATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAAt                                    <  7:34029/149‑1 (MQ=255)
                                                                                                                        gggACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGa                     <  4:63594/149‑1 (MQ=255)
                                                                                                                           aCTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGc                   >  4:114214/1‑148 (MQ=255)
                                                                                                                                ttCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGc             <  5:2585/149‑1 (MQ=255)
                                                                                                                                 tCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCaaa                <  7:20937/145‑1 (MQ=255)
                                                                                                                                 tCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAAACATTAATGACAACATTTTAAGAGCaaa                >  8:20937/1‑145 (MQ=255)
                                                                                                                                          ccttAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGc    <  3:11954/148‑1 (MQ=255)
                                                                                                                                           cttAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCtt  <  8:121033/149‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                    
GAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTT  >  NC_000913/3653091‑3653378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.