Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,832,1880AT22.5% 76.1 / 19.8 40intergenic (+22/+31)yicH/yicIputative inner membrane‑anchored periplasmic AsmA family protein/putative alpha‑glucosidase
Reads supporting (aligned to +/- strand):  ref base A (14/17);  new base T (4/5);  total (18/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACAT  >  NC_000913/3832043‑3832319
                                                                                                                                                 |                                                                                                                                   
gTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCt                                                                                                                                   >  5:121446/1‑148 (MQ=255)
gTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACt                                                                                                                                   >  4:69802/1‑148 (MQ=255)
    aaaCCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGAc                                                                                                                              <  2:2573/149‑1 (MQ=255)
           cGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTACCCg                                                                                                                       <  6:121446/149‑1 (MQ=255)
           cGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCg                                                                                                                       <  2:20304/149‑1 (MQ=255)
             gTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTACCCGGc                                                                                                                     <  6:108830/149‑1 (MQ=255)
                aGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCAt                                                                                                                   <  3:69802/148‑1 (MQ=255)
                                ccGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACa                                                                                                  <  6:37567/149‑1 (MQ=255)
                                       cgccgcAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAAt                                                                                           <  1:171312/149‑1 (MQ=255)
                                                gcagcaAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAgcg                                                                                   >  7:113134/1‑148 (MQ=255)
                                                     aaGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTcc                                                                             <  8:113134/149‑1 (MQ=255)
                                                          aCTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCTGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCtt                                                                          >  8:8680/1‑147 (MQ=255)
                                                              aaaCCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCTGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCt                                                                    <  7:8680/149‑1 (MQ=255)
                                                                    tGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTcacca                                                              >  1:77375/1‑149 (MQ=255)
                                                                             gCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCt                                                     >  5:53066/1‑149 (MQ=255)
                                                                                tCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCt                                                                           <  4:6847/124‑1 (MQ=255)
                                                                                tCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCt                                                                           >  3:6847/1‑124 (MQ=255)
                                                                                   tttCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCAc                                               >  7:154286/1‑149 (MQ=255)
                                                                                       cAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTc                                           >  2:199255/1‑149 (MQ=255)
                                                                                        aGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCa                                          <  6:53066/149‑1 (MQ=255)
                                                                                           ggTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGcc                                       >  1:125304/1‑149 (MQ=255)
                                                                                             tGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTg                                     >  5:115533/1‑149 (MQ=255)
                                                                                               aaGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGc                                    <  8:154286/148‑1 (MQ=255)
                                                                                                      aTCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTc                            <  6:115533/149‑1 (MQ=255)
                                                                                                           cGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCa                       <  2:125304/149‑1 (MQ=255)
                                                                                                                ccGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTc                                                 <  1:140345/118‑1 (MQ=255)
                                                                                                                ccGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTc                                                 >  2:140345/1‑118 (MQ=255)
                                                                                                                        gTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCa                  >  3:35562/1‑141 (MQ=255)
                                                                                                                        gTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCa                  <  4:35562/141‑1 (MQ=255)
                                                                                                                           aaCATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTca        >  2:146852/1‑148 (MQ=255)
                                                                                                                              aTCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCacaac    <  2:77375/149‑1 (MQ=255)
                                                                                                                                 aaTCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACAt  <  1:199255/148‑1 (MQ=255)
                                                                                                                                         cGGGTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCa                       <  2:45690/119‑1 (MQ=255)
                                                                                                                                         cGGGTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCa                       >  1:45690/1‑119 (MQ=255)
                                                                                                                                         cGGGTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGa                     <  5:9960/121‑1 (MQ=255)
                                                                                                                                         cGGGTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGa                     >  6:9960/1‑121 (MQ=255)
                                                                                                                                           ggTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCAt                 >  7:104127/1‑123 (MQ=255)
                                                                                                                                           ggTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCAt                 <  8:104127/123‑1 (MQ=255)
                                                                                                                                           ggTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTc                            >  3:138199/1‑112 (MQ=255)
                                                                                                                                           ggTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTc                            <  4:138199/112‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                   
GTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACAT  >  NC_000913/3832043‑3832319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑