Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,366,8550CA26.5% 91.6 / 30.7 49intergenic (‑82/+36)dcuA/aspAC4‑dicarboxylate antiporter/aspartate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base C (18/18);  new base A (7/6);  total (25/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCAC‑AAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAGA  >  NC_000913/4366713‑4366990
                                                                                                                                               |                                                                                                                                       
cccccAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCAC‑AAGAAAAAAGGCACGTCATCTTACGt                                                                                                                                   >  3:6302/1‑149 (MQ=255)
 ccccAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCAC‑AAGAAAAAAGGCACGTCAGATGACGTg                                                                                                                                  >  6:107340/1‑149 (MQ=255)
  cccAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCAC‑AAGAAAAAAGGCACGTCATCTGACGTGc                                                                                                                                 >  5:53702/1‑149 (MQ=255)
      aTCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCAC‑AAGAAAAAAGGCACGTCAGATGACGTGCCttt                                                                                                                             >  6:146116/1‑149 (MQ=255)
        cTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCAC‑AAGAAAAAAGGCACGTCATCTGACGTGCCttttt                                                                                                                           <  5:21592/149‑1 (MQ=255)
           gcgcCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCAC‑AAGAAAAAAGGCACGTCAGATGACGTGCCttttttc                                                                                                                         <  5:146116/148‑2 (MQ=255)
              cccAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCAC‑AAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTg                                                                                                                     <  1:83096/149‑1 (MQ=255)
                 aagaagATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCAC‑AAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGtac                                                                                                                  <  1:163930/149‑1 (MQ=255)
                   gaagaTCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCAC‑AAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGtacta                                                                                                                >  7:138971/1‑149 (MQ=255)
                      gaTCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCAC‑AAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTAccc                                                                                                             >  6:125182/1‑149 (MQ=255)
                                  aaCTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCAC‑AAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACt                                                                                                 >  3:79328/1‑149 (MQ=255)
                                    cTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAATAAAAAAGGCACGTCAGATGACGTGCCTTTTTTCtt                                                                                                                       <  2:119080/126‑1 (MQ=38)
                                    cTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAATAAAAAAGGCACGTCAGATGACGTGCCTTTTTTCtt                                                                                                                       >  1:119080/1‑126 (MQ=38)
                                    cTATGATGAGTTCAACAACTAGCAAATAAGCCTTCCTTGTTTTTTAACACGTTTATATTAGATTGTTATTTTTAAGTAACTGCTCAC‑AACAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGt                                                                                               <  4:6302/149‑1 (MQ=255)
                                          tgaGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCAC‑AAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCtt                                                                                         <  1:55330/149‑1 (MQ=255)
                                                    gaCTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCAC‑AAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGta                                                                                >  4:141700/2‑148 (MQ=255)
                                                         gCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCAC‑AAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGc                                                                            <  4:79328/147‑1 (MQ=255)
                                                                 gCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCAC‑AAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCttt                                                                  <  3:141700/149‑1 (MQ=255)
                                                                 gCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCAC‑AAGAAAAAAGGCACGTCAGATGACGTGCCTTTTTTCttgt                                                                                                                     >  6:97563/1‑96 (MQ=37)
                                                                 gCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCAC‑AAGAAAAAAGGCACGTCAGATGACGTGCCTTTTTTCttgt                                                                                                                     <  5:97563/98‑3 (MQ=37)
                                                                    ttccttGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCAC‑AAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGt                                                                                               <  7:34220/117‑1 (MQ=255)
                                                                    ttccttGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCAC‑AAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGt                                                                                               >  8:34220/1‑117 (MQ=255)
                                                                                         gATATTAGATTGTTATTTTTAAGTTACTGCTCAC‑AAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCt                                           <  5:125182/148‑1 (MQ=255)
                                                                                             ttAGATTGTTATTTTTAAGTTACTGCTCAC‑AAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGa                                                                                                      >  4:79512/1‑85 (MQ=255)
                                                                                             ttAGATTGTTATTTTTAAGTTACTGCTCAC‑AAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGa                                                                                                      <  3:79512/85‑1 (MQ=255)
                                                                                                     ttATTTTTAAGTTACTGCTCAC‑AAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGt                                                       >  3:106054/1‑124 (MQ=255)
                                                                                                     ttATTTTTAAGTTACTGCTCAC‑AAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGt                                                       <  4:106054/124‑1 (MQ=255)
                                                                                                      tATTTTTAAGTTACTGCTCAC‑AAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCgg                                                         >  7:64410/1‑121 (MQ=255)
                                                                                                      tATTTTTAAGTTACTGCTCAC‑AAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCgg                                                         <  8:64410/121‑1 (MQ=255)
                                                                                                        tttttAAGTTACTGCTCAC‑AAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATc                           <  8:138971/149‑1 (MQ=255)
                                                                                                         ttttAAGTTACTGCTCAC‑AAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCg                          >  7:27454/1‑149 (MQ=255)
                                                                                                            tAAGTTACTGCTCAC‑AAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTc                        <  6:53702/148‑1 (MQ=255)
                                                                                                                    tGCTCAC‑AAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACACTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGc               >  4:33737/1‑149 (MQ=255)
                                                                                                                      cTCAC‑AAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCg                <  7:135588/146‑1 (MQ=255)
                                                                                                                      cTCAC‑AAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCg                >  8:135588/1‑146 (MQ=255)
                                                                                                                      cTCAC‑AAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCt              <  8:27454/148‑1 (MQ=255)
                                                                                                                       tCAC‑AAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGtata                                                                              <  7:11966/83‑1 (MQ=255)
                                                                                                                       tCAC‑AAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGtata                                                                              >  8:11966/1‑83 (MQ=255)
                                                                                                                           aaataaaaaaGGCACGTCAGATGACGTGCCTTTTTTCTTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAg                     <  3:40095/133‑1 (MQ=255)
                                                                                                                           aaataaaaaaGGCACGTCAGATGACGTGCCTTTTTTCTTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAg                     >  4:40095/5‑137 (MQ=255)
                                                                                                                            aaGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGt         >  2:144011/1‑148 (MQ=255)
                                                                                                                               aaaaaaGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTc            >  4:78741/1‑142 (MQ=255)
                                                                                                                               aaaaaaGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTc            <  3:78741/142‑1 (MQ=255)
                                                                                                                                aaaaaGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCaa      >  5:39934/1‑147 (MQ=255)
                                                                                                                                  aaaGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAGa  >  6:176560/1‑149 (MQ=255)
                                                                                                                                  aaaGGCACGTCAGATGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAata                             >  6:69386/1‑122 (MQ=255)
                                                                                                                                  aaaGGCACGTCAGATGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAata                             <  5:69386/122‑1 (MQ=255)
                                                                                                                                        aCGTCAGATTACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGTGTGCATCAGATTCTGTACGGAGAATATATCGTCaa                      <  7:78071/123‑1 (MQ=255)
                                                                                                                                        aCGTCAGATGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTCTACTGAGAAAATATCGTCaa                      >  8:78071/1‑123 (MQ=255)
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CCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCAC‑AAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAGA  >  NC_000913/4366713‑4366990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑