Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913169,0340CA28.3% 193.7 / 78.5 106Y517* (TAC→TAAfhuAferrichrome outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base C (41/35);  new base A (14/16);  total (55/51)
Fisher's exact test for biased strand distribution p-value = 5.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.78e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 108 total aligned reads displayed.

TTCAGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTACCGGAAGATCGTCCGA  >  NC_000913/168889‑169182
                                                                                                                                                 |                                                                                                                                                    
ttCAGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTg                                                                                                                                                   >  6:302428/1‑149 (MQ=255)
   aGGATCAGGCGCAGTGGGATAAAGTGCTGGTCCCCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGttt                                                                                                                                                >  1:103156/1‑149 (MQ=255)
   aGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGtt                                                                                                                                                 <  5:302428/148‑1 (MQ=255)
   aGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGttt                                                                                                                                                <  8:337099/149‑1 (MQ=255)
   aGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGtt                                                                                                                                                 <  5:69594/148‑1 (MQ=255)
          ggCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATg                                                                                                                                         <  7:45787/149‑1 (MQ=255)
          ggCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATg                                                                                                                                         <  4:216718/149‑1 (MQ=255)
            cgcAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGgt                                                                                                                                       <  6:153691/149‑1 (MQ=255)
             gcAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGgtg                                                                                                                                      >  7:217025/1‑149 (MQ=255)
             gcAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACATGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGgtg                                                                                                                                      <  4:161344/149‑1 (MQ=255)
                   ggATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAacac                                                                                                                                >  4:306826/1‑149 (MQ=255)
                     aTAAAGTGCTGGTCACGCTTGGCGGTCGTTATGACTGGGCAGTTCATGAATCTCGTAACCTCGTGTCCGGGACGACCGATAACCGTGATGACAAACAGTTTACCGGGCGTGGTGGTGTTAACTAACTGGGTGATAATGGTGTAACACCt                                                                                                                              <  1:126108/149‑1 (MQ=255)
                     aTAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGCCCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCt                                                                                                                              >  7:11720/1‑149 (MQ=255)
                      tAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCtt                                                                                                                             <  7:367416/149‑1 (MQ=255)
                             cTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAg                                                                                                                      >  6:263659/1‑149 (MQ=255)
                             cTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAg                                                                                                                      <  2:202647/149‑1 (MQ=255)
                               ggTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGc                                                                                                                     >  3:108895/1‑148 (MQ=255)
                                gTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGc                                                                                                                     <  2:270477/147‑1 (MQ=255)
                                    cccTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGAGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGc                                                                                                               >  3:413915/1‑149 (MQ=255)
                                     ccTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGc                                                                                                               >  3:111829/1‑148 (MQ=255)
                                         ggCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTCACTTCAGCTATAGCGAATc                                                                                                          <  3:306826/149‑1 (MQ=255)
                                           cGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGt                                                                                                        <  4:305618/149‑1 (MQ=255)
                                           cGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGt                                                                                                        <  5:263659/149‑1 (MQ=255)
                                             gTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGttt                                                                                                      <  4:111829/149‑1 (MQ=255)
                                                gTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGa                                                                                                    <  6:58020/148‑1 (MQ=255)
                                                  tATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTc                                                                                                                        >  8:413426/1‑126 (MQ=255)
                                                  tATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTc                                                                                                                        <  7:413426/126‑1 (MQ=255)
                                                  tATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAAcc                                                                                                 >  8:158808/1‑149 (MQ=255)
                                                      aCTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGgtgt                                                                                                                                     >  4:235144/1‑109 (MQ=255)
                                                      aCTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGgtgt                                                                                                                                     <  3:235144/109‑1 (MQ=255)
                                                          ggCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCttcttc                                                                                           <  5:421304/147‑1 (MQ=255)
                                                            cAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCaa                                                                                       <  4:231652/149‑1 (MQ=255)
                                                               aTCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACAcc                                                                                                                               <  7:299520/106‑1 (MQ=255)
                                                               aTCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACAcc                                                                                                                               >  8:299520/1‑106 (MQ=255)
                                                                tCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTg                                                                                   >  7:324288/1‑149 (MQ=255)
                                                                 cAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGttt                                                                                                      >  3:93994/1‑129 (MQ=255)
                                                                 cAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGttt                                                                                                      <  4:93994/129‑1 (MQ=255)
                                                                           ttAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGa                                                                            <  7:158808/145‑1 (MQ=255)
                                                                             aaCCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTaa                                                                      >  7:446665/1‑149 (MQ=255)
                                                                             aaCCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTaa                                                                      >  6:98307/1‑149 (MQ=255)
                                                                             aaCCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGAGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATgg                                                                         <  4:413915/146‑1 (MQ=255)
                                                                             aaCCGCCTTGCAGGTAAGACCTATAACCGTGATGACAAACAGTTTACATGGCGTGTTGGTGTTAACTAACTGTTTGATAATGGTGTCACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGTGAAGGATgt                                                                         <  8:217025/146‑2 (MQ=255)
                                                                              aCCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTTGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAat                                                                     <  2:494724/149‑1 (MQ=255)
                                                                               ccGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAata                                                                    >  4:38019/1‑149 (MQ=255)
                                                                                cgcgTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGaa                                                                               <  5:460537/137‑1 (MQ=255)
                                                                                cgcgTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGaa                                                                               >  6:460537/1‑137 (MQ=255)
                                                                                cgcgTTGCCGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATAtt                                                                  >  6:337688/1‑149 (MQ=255)
                                                                                   gTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATAtttt                                                                <  8:446665/149‑1 (MQ=255)
                                                                                            acgacCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCt                                                                                                                                                    <  3:5132/56‑1 (MQ=255)
                                                                                            acgacCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCt                                                                                                                                                    >  4:5132/1‑56 (MQ=255)
                                                                                              gacCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCt                                                      >  8:400030/1‑148 (MQ=255)
                                                                                                ccGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTa                                                     >  6:156078/1‑147 (MQ=255)
                                                                                                 cGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAg                                                  >  1:405322/1‑149 (MQ=255)
                                                                                                 cGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAg                                                  <  7:175031/149‑1 (MQ=255)
                                                                                                   aTAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAAc                                                                                                  <  4:182810/99‑1 (MQ=255)
                                                                                                   aTAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAAc                                                                                                  >  3:182810/1‑99 (MQ=255)
                                                                                                   aTAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATgg                                                                         >  2:42385/1‑124 (MQ=255)
                                                                                                   aTAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATgg                                                                         <  1:42385/124‑1 (MQ=255)
                                                                                                   aTAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGt                                                <  4:108895/149‑1 (MQ=255)
                                                                                                      aaCGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAg                                             >  5:353285/1‑149 (MQ=255)
                                                                                                        cGTGATGACAAACAGTTTTCCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTGCTTCGCAAGTTGGGAAGGATGTTAATATTTTCGCACCGTCTAAAGGTAATCa                                           <  8:11720/149‑1 (MQ=255)
                                                                                                          tgatgaCAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCg                                                                                          >  1:14358/1‑100 (MQ=255)
                                                                                                             tgaCAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATg                                      <  2:405322/149‑1 (MQ=255)
                                                                                                             tgaCAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTAt                                       >  3:379478/1‑148 (MQ=255)
                                                                                                               aCAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATg                                      >  8:430347/1‑147 (MQ=255)
                                                                                                               aCAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGaa                                    <  2:34108/149‑1 (MQ=255)
                                                                                                                 aaaCAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGt                                  >  4:300028/1‑149 (MQ=255)
                                                                                                                 aaaCAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGt                                  >  2:142728/1‑149 (MQ=255)
                                                                                                                 aaaCAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGt                                  >  3:380985/1‑149 (MQ=255)
                                                                                                                  aaCAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTc                                 >  8:351150/1‑149 (MQ=255)
                                                                                                                  aaCAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTc                                 <  7:351150/149‑1 (MQ=255)
                                                                                                                  aaCAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTc                                 >  6:397452/1‑149 (MQ=255)
                                                                                                                   aCAGTTTACCTAGCGTGGAGCTCTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCCATAGAACCTACTTCGCAAGTTGGGAAGGATGGAAATATTTTCGCACCGTCTAAACGAAAGCAGTATGAAGtag                                >  2:353225/1‑147 (MQ=255)
                                                                                                                    cAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAg                                                                                      <  1:255621/94‑1 (MQ=255)
                                                                                                                    cAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAg                                                                                      >  2:255621/1‑94 (MQ=255)
                                                                                                                     aGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGc                              <  3:300028/149‑1 (MQ=255)
                                                                                                                     aGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAg                                                                              >  1:66538/1‑101 (MQ=255)
                                                                                                                     aGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAg                                                                              <  2:66538/101‑1 (MQ=255)
                                                                                                                      gTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCGTACTTCAGCTATAGCGATTCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGGAATATATTCGCACCGTCTAATGGTAAGCAGTATGATGTCggag                             >  1:421536/1‑147 (MQ=255)
                                                                                                                      gTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACATTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCg                             >  1:414663/1‑149 (MQ=255)
                                                                                                                       tttACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGt                            >  7:424221/1‑149 (MQ=255)
                                                                                                                            ctagCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGaaa                        >  5:231072/4‑148 (MQ=255)
                                                                                                                               gtgtggtggtgTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATg                    >  1:265403/3‑149 (MQ=255)
                                                                                                                               gCGTGGTGGTGTTAACTGCCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATg                    >  5:73490/1‑149 (MQ=255)
                                                                                                                                 gtggtggtgTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTa                  >  1:284043/1‑149 (MQ=255)
                                                                                                                                     tggtgTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTACCg               >  7:474940/1‑148 (MQ=255)
                                                                                                                                       gtgtTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTACCGGaa            <  5:337688/149‑1 (MQ=255)
                                                                                                                                       gtgtTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTACCGGaa            <  8:424221/149‑1 (MQ=255)
                                                                                                                                        tgtTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTACCGGAAg           <  6:353285/149‑1 (MQ=255)
                                                                                                                                          ttAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGa                                                                            <  8:225967/82‑1 (MQ=255)
                                                                                                                                          ttAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGa                                                                            >  7:225967/1‑82 (MQ=255)
                                                                                                                                          ttAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATAAAGTCGGCGTGAAATATGTACCgg              >  7:229060/1‑144 (MQ=255)
                                                                                                                                            aactaactGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAAACTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTACCGGAAGATCg       >  1:316088/1‑149 (MQ=255)
                                                                                                                                            aaCTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTAc                 >  8:410283/1‑139 (MQ=255)
                                                                                                                                            aaCTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTAc                 <  7:410283/139‑1 (MQ=255)
                                                                                                                                            aaCTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTACCGGAAGATCg       >  7:96419/1‑149 (MQ=255)
                                                                                                                                              cTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTACCGGAAGATc        <  8:265435/146‑1 (MQ=255)
                                                                                                                                              cTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTACCGGAAGATCGTc     <  2:265403/149‑1 (MQ=255)
                                                                                                                                               tACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTTGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTACCGGAAGATCGTcc    >  3:416272/1‑149 (MQ=255)
                                                                                                                                                 ccTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTACCGGAAGATCGTCCGa  <  4:379478/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TTCAGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTACCGGAAGATCGTCCGA  >  NC_000913/168889‑169182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: