Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,806,335	G→T	intergenic (‑252/‑35)	ydiY ← / → pfkB	acid‑inducible putative outer membrane protein/6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,806,335	0	G	T	100.0%	135.5 / NA	39	intergenic (‑252/‑35)	ydiY/pfkB	acid‑inducible putative outer membrane protein/6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (19/20);  total (19/20)

CGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCG  >  NC_000913/1806188‑1806468                                                                                                                                                    |                                                                                                                                      cgcgAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGC‑AAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGt                                                                                                                                     <  3:266827/149‑1 (MQ=255)  gcgAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGt                                                                                                                                     <  4:9124/149‑1 (MQ=255)  gcgAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGt                                                                                                                                     <  7:474006/149‑1 (MQ=255)  gcgAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGt                                                                                                                                     <  7:406586/149‑1 (MQ=255)    gAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGTCGAAAACTGATTTATATTATTATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCtgtgg                                                                                                                                   <  5:405313/149‑2 (MQ=255)        aaCTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTATTCACTTTCCGCTGATTCTGTGACAGa                                                                                                                               >  5:462445/1‑149 (MQ=255)         aCTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGAc                                                                                                                              <  5:274454/149‑1 (MQ=255)               cAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAAt                                                                                                                        >  6:270408/1‑149 (MQ=255)                    tATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGc                                                                                                                    >  4:274050/1‑148 (MQ=255)                     aTTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGcc                                                                                                                   >  3:430514/1‑148 (MQ=255)                     aTTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCAAATAAATAATATTGTTAATTTCTTCAATTTACGATGATTCTGTGCAAGACTGAAATAAGcc                                                                                                                   >  7:177685/1‑148 (MQ=255)                                aTTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGaaa                                                                                                       >  7:272984/1‑149 (MQ=255)                                  tttttGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATagga                                                                                                            <  2:144735/142‑1 (MQ=255)                                  tttttGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGACAGACTGAAATCAGACTATagga                                                                                                            >  1:144735/1‑142 (MQ=255)                                    tttGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCta                                                                                                                 >  4:283012/1‑135 (MQ=255)                                    tttGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCta                                                                                                                 <  3:283012/135‑1 (MQ=255)                                      tGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAatgatg                                                                                                  <  8:272984/148‑1 (MQ=255)                                       gAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATgg                                                                                                 >  5:346176/1‑148 (MQ=255)                                                      gAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGtt                                                                                 <  4:430514/149‑1 (MQ=255)                                                                       tatatataTTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCctctc                                                                >  6:425863/1‑149 (MQ=255)                                                                         tatatatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCctctct                                                               <  5:270408/148‑1 (MQ=255)                                                                           tatatTTATCTGCAAAATTTTAAATAAAGCTCAAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGa                                                            <  6:180555/149‑1 (MQ=255)                                                                            atatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGa                                                            >  3:258017/1‑148 (MQ=255)                                                                              atTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAg                                                         >  1:28935/1‑149 (MQ=255)                                                                               tttATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAgc                                                        <  4:258017/149‑1 (MQ=255)                                                                                          aaTTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTACGCTGATTCTGTGCCAGACTGAAATCAGCCTATATGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTAc                                             <  8:177685/149‑1 (MQ=255)                                                                                                    aaaGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAAtt                                   >  7:305609/1‑149 (MQ=255)                                                                                                        cTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGAGCAACAATTACCCCGCAAATTTATc                               >  7:145567/1‑149 (MQ=255)                                                                                                           cAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATccc                             >  3:173329/1‑148 (MQ=255)                                                                                                            aaTAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCATCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGGCCTCTCTCGATAGCGAAAAAATTACCCCTCAAATTTATCCCg                            >  3:246849/1‑148 (MQ=255)                                                                                                             aTAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGaa                          <  8:305609/149‑1 (MQ=255)                                                                                                             aTAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCACTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGaa                          <  6:346176/149‑1 (MQ=255)                                                                                                                    atatTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAAc                   >  7:492601/1‑149 (MQ=255)                                                                                                                         gTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTgcg               <  4:173329/148‑1 (MQ=255)                                                                                                                          ttAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCt             >  3:95738/1‑149 (MQ=255)                                                                                                                             aTTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGt           <  2:28935/148‑1 (MQ=255)                                                                                                                             aTTTCATAACTTTCCGCTGATTCTGTGCCAGACTGAAATAAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGATGTa          <  6:462445/149‑1 (MQ=255)                                                                                                                                     aCTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCg  <  1:189316/149‑1 (MQ=255)                                                                                                                                      cTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGt           >  4:309077/1‑139 (MQ=255)                                                                                                                                      cTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGt           <  3:309077/139‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                      CGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCG  >  NC_000913/1806188‑1806468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑