Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,183,106 C→T S621F (TCC→TTC)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,183,1060CT100.0% 555.9 / NA 155S621F (TCC→TTC) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (72/83);  total (72/83)

Only 100 of 155 total aligned reads displayed.

AACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCC  >  NC_000913/4182961‑4183250
                                                                                                                                                 |                                                                                                                                                
aaCTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCaa                                                                                                                                               <  7:65736/149‑1 (MQ=255)
aaCTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCaa                                                                                                                                               <  6:32737/149‑1 (MQ=255)
    ctctGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTg                                                                                                                                           >  4:358249/1‑149 (MQ=255)
       tGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTgg                                                                                                                                          <  2:143682/147‑1 (MQ=255)
       tGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTg                                                                                                                                           <  8:50084/146‑1 (MQ=255)
         tCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGa                                                                                                                                         <  3:1917/146‑1 (MQ=255)
         tCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGAt                                                                                                                                        <  7:305265/147‑1 (MQ=255)
            gtgtACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATgaaga                                                                                                                                   <  2:319582/149‑1 (MQ=255)
            gtgtACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATgaag                                                                                                                                    >  2:103530/1‑148 (MQ=255)
               tACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGaagaag                                                                                                                                 <  1:223599/148‑1 (MQ=255)
                 cGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGc                                                                                                                               <  3:358249/148‑1 (MQ=255)
                     caGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAgg                                                                                                                                >  6:149376/1‑143 (MQ=255)
                     caGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAgg                                                                                                                                <  5:149376/143‑1 (MQ=255)
                      aGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTc                                                                                                                         >  7:335633/1‑149 (MQ=255)
                      aGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGAGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTc                                                                                                                         >  2:342051/1‑149 (MQ=255)
                          tAACGAATACTGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACCGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAATAAGGCAACTACCTTATCGCCCAGGCGAACATCAAATTGGATGAAGAAGGCCACTTct                                                                                                                        >  2:408570/1‑145 (MQ=255)
                          tAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCATGCGAACTTCAACTTGGATGAATCAGGCCACTTct                                                                                                                        >  6:139409/1‑145 (MQ=255)
                            aCGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTagaa                                                                                                                   <  4:367600/149‑1 (MQ=255)
                            aCGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTagaa                                                                                                                   <  2:459562/149‑1 (MQ=255)
                            aCGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTag                                                                                                                     <  8:180695/147‑1 (MQ=255)
                            aCGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTag                                                                                                                     >  1:265262/1‑147 (MQ=255)
                             cGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTag                                                                                                                     >  3:221577/1‑146 (MQ=255)
                              gAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTagaaga                                                                                                                 <  1:103530/149‑1 (MQ=255)
                                aTACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGAcc                                                                                                               <  1:408570/149‑1 (MQ=255)
                                      cTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTaa                                                                                                         <  4:163936/149‑1 (MQ=255)
                                           ttGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGc                                                                                                    >  2:160837/1‑149 (MQ=255)
                                              agaCTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCg                                                                                                  <  8:222847/148‑1 (MQ=255)
                                                 cTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGc                                                                                              <  5:240129/149‑1 (MQ=255)
                                                  tCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGc                                                                                              >  7:49477/1‑148 (MQ=255)
                                                   ccGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCa                                                                                             >  8:475209/1‑148 (MQ=255)
                                                    cGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCaaa                                                                                           <  7:202211/149‑1 (MQ=255)
                                                    cGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCaaa                                                                                           <  4:73046/149‑1 (MQ=255)
                                                        tCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGc                                                                                        <  7:172450/148‑1 (MQ=255)
                                                        tCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGc                                                                                        >  5:359849/1‑148 (MQ=255)
                                                         cGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAg                                                                                          <  6:287464/145‑1 (MQ=255)
                                                         cGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAg                                                                                          >  5:287464/1‑145 (MQ=255)
                                                           tAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAAt                                                                                    >  7:249716/1‑149 (MQ=255)
                                                            aaaGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCaa                                                                                            >  6:395327/1‑140 (MQ=255)
                                                              aGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCa                                                                                 <  2:265262/149‑1 (MQ=255)
                                                                      aCGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTc                                                                         <  8:294286/149‑1 (MQ=255)
                                                                       cGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCa                                                                        <  4:82403/149‑1 (MQ=255)
                                                                       cGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCa                                                                        <  1:160837/149‑1 (MQ=255)
                                                                          tgttgtAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGcc                                                                     <  7:475209/149‑1 (MQ=255)
                                                                              gtAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCt                                                                              >  6:323249/1‑136 (MQ=255)
                                                                              gtAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGt                                                                           <  8:37332/139‑1 (MQ=255)
                                                                              gtAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGt                                                                           >  7:37332/1‑139 (MQ=255)
                                                                               tAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCtt                                                                             >  1:226576/1‑136 (MQ=255)
                                                                               tAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCtt                                                                             <  2:226576/136‑1 (MQ=255)
                                                                                 aCTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCa                                                              <  6:336809/149‑1 (MQ=255)
                                                                                   tGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTagaa                                                                                                                   <  1:446053/94‑1 (MQ=255)
                                                                                    gACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGt                                                           >  8:50120/1‑149 (MQ=255)
                                                                                      cGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAgg                                                            <  2:7419/146‑1 (MQ=255)
                                                                                      cGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAgg                                                            >  1:7419/1‑146 (MQ=255)
                                                                                       gAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATg                                                                                                                                       >  7:70034/1‑70 (MQ=255)
                                                                                       gAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCt                                                                                                              >  2:160684/1‑95 (MQ=255)
                                                                                       gAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGa                                                        >  6:111344/1‑149 (MQ=255)
                                                                                       gAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGa                                                        >  7:131472/1‑149 (MQ=255)
                                                                                       gAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGa                                                        >  3:114347/1‑149 (MQ=255)
                                                                                       gAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGCACACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGAGAATCCAGCTTGTTCAGCCGCGACCAGGTTGa                                                        >  1:481051/1‑149 (MQ=255)
                                                                                        aaaTTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGAc                                                       <  2:425392/149‑1 (MQ=255)
                                                                                           ttCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTAc                                                    <  4:221577/149‑1 (MQ=255)
                                                                                            tCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGc                                                                                        <  3:26853/112‑1 (MQ=255)
                                                                                            tCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGc                                                                                        >  4:26853/1‑112 (MQ=255)
                                                                                             cACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACa                                                   <  8:249716/148‑1 (MQ=255)
                                                                                                   ccgTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGt                                            <  8:115901/147‑1 (MQ=255)
                                                                                                   cTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGa                                                        <  4:54798/137‑1 (MQ=255)
                                                                                                   cTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGa                                                        >  3:54798/1‑137 (MQ=255)
                                                                                                   cTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACg                                             >  8:264452/1‑148 (MQ=255)
                                                                                                     gTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTa                                                     >  3:383177/1‑138 (MQ=255)
                                                                                                     gTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGAc                                              <  2:212250/145‑1 (MQ=255)
                                                                                                     gTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTAt                                          >  7:416183/1‑149 (MQ=255)
                                                                                                      tCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTa                                           <  8:6715/147‑1 (MQ=255)
                                                                                                      tCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATc                                         <  6:223093/149‑1 (MQ=255)
                                                                                                      tCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATc                                         >  1:276596/1‑149 (MQ=255)
                                                                                                        tGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGt                                                                                                 <  1:145384/91‑1 (MQ=255)
                                                                                                        tGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAg                                                             <  8:434498/127‑1 (MQ=255)
                                                                                                          cTATCGAAGAAGGCAACTACGTTATCGCGCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCTAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCAcc                                     >  7:431944/1‑149 (MQ=255)
                                                                                                          cTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATcc                                        <  5:230577/146‑1 (MQ=255)
                                                                                                           tATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCAcc                                     >  8:385722/1‑148 (MQ=255)
                                                                                                           tATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCAcc                                     >  5:358898/1‑148 (MQ=255)
                                                                                                           tATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCAcc                                     >  1:405240/1‑148 (MQ=255)
                                                                                                                aagaagGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGTCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCcagcag                               >  5:122568/1‑149 (MQ=255)
                                                                                                                aagaagGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTa                                                     >  6:433237/1‑127 (MQ=255)
                                                                                                                aagaagGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTa                                                     <  5:433237/127‑1 (MQ=255)
                                                                                                                aagaagGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCAcc                                     <  8:332791/143‑1 (MQ=255)
                                                                                                                 agaagGCAACTACGTTATCTCCCAGGCGAACTTCAACTTGGATGaagaag                                                                                                                                 >  8:426756/1‑50 (MQ=255)
                                                                                                                 agaagGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGaagaag                                                                                                                                 <  7:426756/50‑1 (MQ=255)
                                                                                                                 agaagGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAgg                              <  4:114347/149‑1 (MQ=255)
                                                                                                                   aagGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAggtg                            <  5:111344/149‑1 (MQ=255)
                                                                                                                      gCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTa                         <  8:416183/149‑1 (MQ=255)
                                                                                                                        aaCTACGTTATCTCCCATGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGcc                                                                     <  6:195195/103‑1 (MQ=255)
                                                                                                                        aaCTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGcc                                                                     >  5:195195/1‑103 (MQ=255)
                                                                                                                        aaCTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATc                       >  8:168296/1‑149 (MQ=255)
                                                                                                                        aaCTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATc                       >  8:16006/1‑149 (MQ=255)
                                                                                                                             cGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGt                    <  7:385914/147‑1 (MQ=255)
                                                                                                                                  tCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCg             >  8:191494/1‑149 (MQ=255)
                                                                                                                                     cccAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTcc          >  5:31702/1‑149 (MQ=255)
                                                                                                                                      ccAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTccc         <  1:186622/149‑1 (MQ=255)
                                                                                                                                      ccAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTccc         <  5:301057/149‑1 (MQ=255)
                                                                                                                                             aaCTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATccc  <  6:358898/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCC  >  NC_000913/4182961‑4183250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: