Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,806,342	G→T	intergenic (‑259/‑28)	ydiY ← / → pfkB	acid‑inducible putative outer membrane protein/6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,806,342	0	G	T	100.0%	167.6 / NA	48	intergenic (‑259/‑28)	ydiY/pfkB	acid‑inducible putative outer membrane protein/6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (23/25);  total (23/25)

TTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGG  >  NC_000913/1806198‑1806483                                                                                                                                                 |                                                                                                                                              ttAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAACTTCTTCACTTTCCGCTGATTCGGTGCCATACt                                                                                                                                            >  6:93260/1‑148 (MQ=255)    gCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGaaa                                                                                                                                        >  4:80298/1‑149 (MQ=255)    gCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGaa                                                                                                                                         <  2:154805/148‑1 (MQ=255)               ttATTTCATTTTTTGAATAAGCATGTCGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTATCCTCTGATTCGGTGCCATACTGATATCAGCCTATag                                                                                                                             >  1:134268/1‑149 (MQ=255)                   ttCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGc                                                                                                                                   <  8:100405/139‑1 (MQ=255)                   ttCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGc                                                                                                                                   >  7:100405/1‑139 (MQ=255)                     cATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAggagga                                                                                                                        >  3:42203/1‑148 (MQ=255)                      aTTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCAGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGaaa                                                                                                                      >  3:149866/1‑149 (MQ=255)                         ttttGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAatga                                                                                                                   >  8:6997/1‑149 (MQ=255)                          tttGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAACTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAatga                                                                                                                   <  5:93260/148‑1 (MQ=255)                           ttGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAatgat                                                                                                                  <  5:147031/148‑1 (MQ=255)                             gAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATgg                                                                                                                <  5:114816/148‑1 (MQ=255)                              aaTAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGTa                                                                                                              >  5:98865/1‑149 (MQ=255)                               aTAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGTAc                                                                                                             <  8:150974/149‑1 (MQ=255)                                   gCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTAt                                                                                                         <  4:56644/149‑1 (MQ=255)                                   gCATGTGGCGAAAACAGATTCTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTAt                                                                                                         <  3:142945/149‑1 (MQ=255)                                         ggCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATAc                                                                                                   >  1:103985/1‑149 (MQ=255)                                            gAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAatgatg                                                                                                                 <  1:86261/132‑1 (MQ=255)                                            gAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAatgatg                                                                                                                 >  2:86261/1‑132 (MQ=255)                                                 cAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGacac                                                                                           <  4:149866/149‑1 (MQ=255)                                                   gATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACtt                                                                                         <  6:98865/149‑1 (MQ=255)                                                               tatatatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCtctctc                                                                             <  4:42203/149‑1 (MQ=255)                                                                       tATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAgcg                                                                      <  2:103985/148‑1 (MQ=255)                                                                          cTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTgc                                                                                       <  6:2904/128‑1 (MQ=255)                                                                          cTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTgc                                                                                       >  5:2904/1‑128 (MQ=255)                                                                           tGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATagga                                                                                                                           >  6:129081/1‑91 (MQ=255)                                                                           tGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATagga                                                                                                                           <  5:129081/91‑1 (MQ=255)                                                                             cAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGcaac                                                                  <  3:80298/146‑1 (MQ=255)                                                                              aaaaTTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAAt                                                               >  7:17583/1‑148 (MQ=255)                                                                              aaaaTTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAAt                                                               >  5:71946/1‑148 (MQ=255)                                                                              aaaaTTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAAt                                                               >  2:69881/1‑148 (MQ=255)                                                                               aaaTTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTa                                                             <  1:69881/149‑1 (MQ=255)                                                                                                 cAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGt                                                                                                               >  2:199346/1‑81 (MQ=255)                                                                                                 cAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGt                                                                                                               <  1:199346/81‑1 (MQ=255)                                                                                                 cAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGtt                                                                                                <  3:150959/96‑1 (MQ=255)                                                                                                 cAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGtt                                                                                                >  4:150959/1‑96 (MQ=255)                                                                                                        tCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGacac                                                                                           >  3:38105/1‑94 (MQ=255)                                                                                                        tCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGacac                                                                                           <  4:38105/94‑1 (MQ=255)                                                                                                               gTTAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTgcgc                             >  8:139020/1‑149 (MQ=255)                                                                                                                ttAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAgcgc                                                                     <  1:142907/108‑1 (MQ=255)                                                                                                                ttAATTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAgcgc                                                                     >  2:142907/1‑108 (MQ=255)                                                                                                                  aaTTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTg                           <  6:71946/148‑1 (MQ=255)                                                                                                                  aaTTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTg                           >  7:109417/1‑148 (MQ=255)                                                                                                                  aaTTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTg                           <  8:17583/148‑1 (MQ=255)                                                                                                                   aTTTCTTCACTTTCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTa                         >  5:185786/1‑149 (MQ=255)                                                                                                                              ttCCGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGgtg              >  3:107493/1‑149 (MQ=255)                                                                                                                                 cGCTGATTCGGTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGtt            <  8:109417/148‑1 (MQ=255)                                                                                                                                          ggTGCCATACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCggg  <  6:185786/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                              TTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGG  >  NC_000913/1806198‑1806483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑