Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,184,543	C→A	P1100Q (CCG→CAG)	rpoB →	RNA polymerase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,184,543	0	C	A	100.0%	353.4 / NA	97	P1100Q (CCG→CAG)	rpoB	RNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (52/45);  total (52/45)

TATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGC  >  NC_000913/4184399‑4184687                                                                                                                                                 |                                                                                                                                                  tataTCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTgg                                                                                                                                              <  4:194693/150‑1 (MQ=255) tataTCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATCGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTgg                                                                                                                                              <  4:521958/150‑1 (MQ=255)    atCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGCTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCg                                                                                                                                           <  1:610920/150‑1 (MQ=255)         gCGGTTAAACGCCGCATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCg                                                                                                                                      >  4:178351/1‑150 (MQ=255)           ggTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑tc                                                                                                                                   >  4:555462/1‑150 (MQ=255)           ggTGAAGCGCCGTATCCCGCCGGGTGGCAAGGTGGCAGGTCGTCACGGTAGCAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATGTGCCTTACGATGAGAGCGGTACTCCGGTAGGCATCGTACTGAACCAGCTGGGCGTACCG‑tc                                                                                                                                   <  2:45516/150‑1 (MQ=255)            attAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑tct                                                                                                                                  <  1:400718/149‑1 (MQ=255)             ttAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑tctc                                                                                                                                 >  2:886747/1‑150 (MQ=255)              tAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTAGGCGTACCG‑TCTCg                                                                                                                                >  4:457438/1‑150 (MQ=255)               aaaCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGt                                                                                                                               <  2:636167/150‑1 (MQ=255)                aaCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTa                                                                                                                              <  3:440922/150‑1 (MQ=255)                 aCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTAt                                                                                                                             >  2:354972/1‑150 (MQ=255)                 aCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTAt                                                                                                                             <  3:2625/150‑1 (MQ=255)                  cGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATg                                                                                                                            <  4:132482/150‑1 (MQ=255)                   gCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGa                                                                                                                           >  1:756571/1‑150 (MQ=255)                    ccGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGaa                                                                                                                          >  4:179325/1‑150 (MQ=255)                    ccGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGaa                                                                                                                          >  2:56440/1‑150 (MQ=255)                         tCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCg                                                                                                                     <  4:138707/150‑1 (MQ=255)                         tCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGAAGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCg                                                                                                                     >  4:537824/1‑150 (MQ=255)                           cAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGt                                                                                                                   <  2:918089/150‑1 (MQ=255)                            aGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTc                                                                                                                  <  1:597721/150‑1 (MQ=255)                             gCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCa                                                                                                                 >  2:772246/1‑150 (MQ=255)                                tGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGAt                                                                                                              >  1:393806/1‑150 (MQ=255)                                 ggTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGAGCGCG‑ATGAACATCGGTCAGATc                                                                                                             >  1:339936/1‑150 (MQ=255)                                 ggTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATc                                                                                                             >  1:43780/1‑150 (MQ=255)                                    gACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTc                                                                                                          >  2:18051/1‑150 (MQ=255)                                       aaGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGaa                                                                                                       >  2:332448/1‑150 (MQ=255)                                       aaGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGaa                                                                                                       <  1:845388/150‑1 (MQ=255)                                          aTGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGCTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAAcc                                                                                                    >  1:812897/1‑150 (MQ=255)                                          aTGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAAcc                                                                                                    <  2:1111286/150‑1 (MQ=255)                                             gCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCAc                                                                                                 >  4:286440/1‑150 (MQ=255)                                               aGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAGGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCt                                                                                               <  2:403681/150‑1 (MQ=255)                                               aGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCt                                                                                               <  1:117036/150‑1 (MQ=255)                                                 gtcgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTgg                                                                                             >  3:556651/1‑150 (MQ=255)                                                 gtcgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTgg                                                                                             >  3:509239/1‑150 (MQ=255)                                                 gtcgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTgg                                                                                             <  4:346939/150‑1 (MQ=255)                                                 gtcgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTgg                                                                                             <  2:472588/150‑1 (MQ=255)                                                 gtcgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTgg                                                                                             >  1:245184/1‑150 (MQ=255)                                                    gtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTa                                                                                          <  1:209393/150‑1 (MQ=255)                                                    gtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCCGGGTa                                                                                          >  2:253794/1‑150 (MQ=255)                                                              cAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaa                                                                                >  2:855554/1‑150 (MQ=255)                                                               aaGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                               >  1:100584/1‑150 (MQ=255)                                                               aaGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                               <  1:198923/150‑1 (MQ=255)                                                                aGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAg                                                                              <  4:515320/150‑1 (MQ=255)                                                                aGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAg                                                                              <  1:1123797/150‑1 (MQ=255)                                                                  ggTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGt                                                                            >  1:979850/1‑150 (MQ=255)                                                                    tgtAATTTCAAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑CCGCGTATGAACATCAGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTAt                                                                          >  2:428310/1‑150 (MQ=255)                                                                      tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCTGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCg                                                                        >  3:454923/1‑150 (MQ=255)                                                                      tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGACCCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCg                                                                        >  4:213517/1‑150 (MQ=255)                                                                           tCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGAc                                                                   >  3:223570/1‑150 (MQ=255)                                                                             tAAGACCAGCCCTATCGAAGATATGCATTACGATGAAAACGGTACGCCGGTAGACTTCGTACTGCACCAGCTGGGCATACCG‑TCTCGTATGAACATCGGTCAGATCCGCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACaa                                                                 <  2:252004/150‑1 (MQ=255)                                                                              aaGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAg                                                                >  2:796348/1‑150 (MQ=255)                                                                                 aTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATc                                                             >  1:414658/1‑150 (MQ=255)                                                                                    aaCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc                                                          <  1:255046/150‑1 (MQ=255)                                                                                      cccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGc                                                        >  1:1006157/1‑150 (MQ=255)                                                                                         gATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGc                                                                                                                                            >  4:261463/1‑64 (MQ=255)                                                                                         gATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGc                                                                                                                                            <  3:261463/64‑1 (MQ=255)                                                                                             gAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATAGGCAAGTAGATCAACGCCATGCTg                                                 >  3:142064/1‑150 (MQ=255)                                                                                              aaGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGa                                                >  4:431261/1‑150 (MQ=255)                                                                                                    tGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagc                                          <  1:285647/150‑1 (MQ=255)                                                                                                     gCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca                                         <  1:755725/150‑1 (MQ=255)                                                                                                     gCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTAGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca                                         <  1:786680/150‑1 (MQ=255)                                                                                                       cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACagcagc                                       >  3:46974/1‑150 (MQ=255)                                                                                                       cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACagcagc                                       >  2:358239/1‑150 (MQ=255)                                                                                                       cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACagcagc                                       >  3:372046/1‑150 (MQ=255)                                                                                                         tACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaa                                     <  2:727144/150‑1 (MQ=255)                                                                                                         tACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaa                                     >  1:42437/1‑150 (MQ=255)                                                                                                          aCGATTAAGACGGTACGCCGGTAGACATCGTACGGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaag                                    <  1:1209973/150‑1 (MQ=255)                                                                                                          aCGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaag                                    >  2:509582/1‑150 (MQ=255)                                                                                                             aTGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaag                                 <  2:245184/150‑1 (MQ=255)                                                                                                              tGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGt                                <  1:680537/150‑1 (MQ=255)                                                                                                                aaaaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcg                              >  4:195731/1‑150 (MQ=255)                                                                                                                 aaaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcgc                             >  3:34162/1‑150 (MQ=255)                                                                                                                 aaaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGGATGGCTGCGAAAGGGAGCGGCGACAAGATCAACGCCATGCTGACACAGCAGCAAGAAGTcgc                             >  1:899326/1‑150 (MQ=255)                                                                                                                  aaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAACCAGCAGCAAGAAGTcgcg                            >  2:770832/1‑150 (MQ=255)                                                                                                                  aaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcgcg                            <  2:959611/150‑1 (MQ=255)                                                                                                                  aaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCGCGAAACCCACCTGGGTATGGCTGCGAAAGGGATCGGAGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcccg                            >  4:442248/1‑150 (MQ=255)                                                                                                                         cGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGATAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgc                     <  1:938224/150‑1 (MQ=255)                                                                                                                         cGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgc                     <  4:27532/150‑1 (MQ=255)                                                                                                                         cGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgc                     <  2:506886/150‑1 (MQ=255)                                                                                                                            cGGTAGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACAACGGGCAGATCCTAGAAAGCCACCTGAGTATGGCTGCGAAAGGTATCTGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGACGCGGAACTGcgcg                  >  4:252286/1‑150 (MQ=255)                                                                                                                               gaGACATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAt               <  4:346762/149‑1 (MQ=255)                                                                                                                                  aCATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCa            <  1:240743/150‑1 (MQ=255)                                                                                                                                   cATCGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCAt           >  2:48563/1‑150 (MQ=255)                                                                                                                                      cGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCa        <  2:727140/150‑1 (MQ=255)                                                                                                                                      cGTACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACACCGGTCAGATCCTCGAATCCCACCTGGGTATTGCTGCGAAAGGTATCGGCGACAAGATCATCGCCATGCTGAAACAGCAGCAATAAGTCGCGAAACTGCGCGAATTCATCCa        <  1:1263175/150‑1 (MQ=255)                                                                                                                                       gTACTGAACCAGCTGGGCGTACCG‑TCGCGTATGAACATCGGTCAGATCCTCGATACCCACCTGGGTATGGCTGCGAAAGGTATCTGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAACTCGCGAAACTGCGCGAATTTATCCAg       <  2:1263319/150‑1 (MQ=255)                                                                                                                                        tACTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAgc      <  1:886747/150‑1 (MQ=255)                                                                                                                                         aCTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCattt     >  3:302299/1‑147 (MQ=255)                                                                                                                                          cTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAgcgt    <  3:179325/150‑1 (MQ=255)                                                                                                                                          cTGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAgcgt    <  2:486937/150‑1 (MQ=255)                                                                                                                                           tGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAgcgtg   >  1:983366/1‑150 (MQ=255)                                                                                                                                           tGAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAgcgtg   >  1:4163/1‑150 (MQ=255)                                                                                                                                            gAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAgcgtgc  >  1:1040925/1‑150 (MQ=255)                                                                                                                                            gAACCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAACAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAgcgtgc  >  4:242329/1‑150 (MQ=255)                                                                                                                                             aaCCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAgc      <  2:579982/145‑1 (MQ=255)                                                                                                                                             aaCCAGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAgc      >  1:579982/1‑145 (MQ=255)                                                                                                                                                 |                                                                                                                                                  TATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCG‑TCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGC  >  NC_000913/4184399‑4184687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑