New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3262188	37 (1.030)	4 (0.120)	4/236	3.5	10.6%	coding (231/2295 nt)	tdcE	pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase
	?	NC_000913	= 3262210	33 (0.990)					coding (209/2295 nt)	tdcE	pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ATGAACTTTTAATCATATTAATGCCACCGAACGGGTGTAGCGCACGTTTCAACGGCGCATCCGTTTGCAGGCCAACAATTTTTTCCAGCGGCTGGTTAATATATCCCGCATCATGAGCGGTAATTGTGGTGGCAATATTGGTATCGAAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3262040‑3262188 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gtatcgaaaATGCAACCCACGCGCCGGTTGATTTCGATACCAATATTGCCACCACAATTACCGCTCATGATGCGGGAT  <  NC_000913/3262210‑3262142                                                                                                                                                                                                                            ATGAACTTTTAATCATATTAATGACACCGAACGGGTGTAGCGCACGTTTCAACGGCGCATCCGTTTGCAGGCCAACAATTTTTTCCAGCGGCTGGTTAATATATCCCGCATCATGAGCGGTAATTGTGGTGGCAATAATGGTATCTAAA                                                                       >  1:145108/1‑149                 ATTAATGCCACCGAACGGGTGTAGCGCACGTTTCAACGGCGCATCCGTTTGCAGGCCAACAATTTTTTCCAGCGGCTGGTTAATATATCCCGCATCATGAGCGGTAATTGTGGTGGCAATATTGGTATCGAAAATGCAACCCACGCGCC                                                       >  2:96962/1‑149                                  GGTGTAGCGCACGTTTCAACGGCGCATCCGTTTGCAGGCCAACAATTTTTTCCAGCGGCTGGTTAATATATCCCGCATCATGAGCGGTAATTGTGGTGGCAATATTGGTATCGAAAATGCAACCCACGCGCCGGTTGATTTCGATACCA                                      <  1:96962/149‑1                                      TAGCGCACGTTTCAACGGCGCATCCGTTTGCAGGCCAACAATTTTTTCCAGCGGCTGGTTAATATATCCCGCATCATGAGCGGTAATTGTGGTGGCAATATTGGTATCGAAAATGCAACCCACGCGCCGGTTGATTTCGATACCAA                                     <  7:134576/146‑1                                      TAGCGCACGTTTCAACGGCGCATCCGTTTGCAGGCCAACAATTTTTTCCAGCGGCTGGTTAATATATCCCGCATCATGAGCGGTAATTGTGGTGGCAATATTGGTATCGAAAATGCAACCCACGCGCCGGTTGATTTCGATACCAA                                     >  8:134576/1‑146                                           CACGTTTCAACGGCGCATCCGTTTGCAGGCCAACAATTTTTTCCAGCGGCTGGTTAATATATCCCGCATCATGAGCGGTAATTGTGGTGGCAATATTGGTATCGAAA                                                                       <  1:75694/107‑1                                           CACGTTTCAACGGCGCATCCGTTTGCAGGCCAACAATTTTTTCCAGCGGCTGGTTAATATATCCCGCATCATGAGCGGTAATTGTGGTGGCAATATTGGTATCGAAA                                                                       >  2:75694/1‑107                                                                                                                                               ATCGAAAATGCAACCCACGCGCCGGTTGATTTCGATACCAATATTGCCACCACAATTACCGCTCATGATGC       <  7:37523/71‑1                                                                                                                                               ATCGAAAATGCAACCCACGCGCCGGTTGATTTCGATACCAATATTGCCACCACAATTACCGCTCATGATGC       >  8:37523/1‑71                                                                                                                                                  GAAAATGCAACCCACGCGCCGGTTGATTTCGATACCAATATTGCCACCACAATTACCGCTCATGATGCGGGAT  >  3:131806/1‑73                                                                                                                                                  GAAAATGCAACCCACGCGCCGGTTGATTTCGATACCAATATTGCCACCACAATTACCGCTCATGATGCGGGAT  <  4:131806/73‑1                                                                                                                                                                                                                            ATGAACTTTTAATCATATTAATGCCACCGAACGGGTGTAGCGCACGTTTCAACGGCGCATCCGTTTGCAGGCCAACAATTTTTTCCAGCGGCTGGTTAATATATCCCGCATCATGAGCGGTAATTGTGGTGGCAATATTGGTATCGAAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3262040‑3262188 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gtatcgaaaATGCAACCCACGCGCCGGTTGATTTCGATACCAATATTGCCACCACAATTACCGCTCATGATGCGGGAT  <  NC_000913/3262210‑3262142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.