Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,183,3451.T21.1% 91.4 / 33.6 38intergenic (+22/+468)zupT/ribBzinc transporter/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base . (15/15);  new base T (4/4);  total (19/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GAATTAATGCCGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTT  >  NC_000913/3183204‑3183484
                                                                                                                                              |                                                                                                                                           
gaATTAATGCCGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCatgcgctg                                                                                                                                       >  3:53828/1‑142 (MQ=255)
 aaTTAATGCCGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATgg                                                                                                                                     <  5:77086/149‑1 (MQ=255)
  aTTAATGCCGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGa                                                                                                                                    <  1:34496/149‑1 (MQ=255)
      aTGCCGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACaa                                                                                                                                 <  7:100533/148‑1 (MQ=255)
         ccGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACtt                                                                                                                              <  2:83384/148‑1 (MQ=255)
         ccGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTc                                                                                                                             >  1:152100/1‑149 (MQ=255)
                  aaaGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCt                                                                                                                     <  6:113487/148‑1 (MQ=255)
                  aaaGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCt                                                                                                                     >  5:113487/1‑148 (MQ=255)
                    agagATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGC‑TGGACAAc                                                                                                                                >  1:167773/1‑135 (MQ=255)
                    agagATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGC‑TGGACAAc                                                                                                                                <  2:167773/135‑1 (MQ=255)
                           gACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTc                                                                                                                   <  7:63490/141‑1 (MQ=255)
                           gACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTc                                                                                                                   >  8:63490/1‑141 (MQ=255)
                              ccTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAAc                                                                                                                                <  6:19966/125‑1 (MQ=255)
                                    aataaCCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGC‑TGGACAACTTc                                                                                                                             <  6:97382/122‑1 (MQ=255)
                                    aataaCCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGC‑TGGACAACTTc                                                                                                                             >  5:97382/1‑122 (MQ=255)
                                     ataaCCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTAc                                                                                                 <  2:152100/149‑1 (MQ=255)
                                         ccccAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCttt                                                                                             <  2:43365/149‑1 (MQ=255)
                                                        aCTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctcttct                                                                              >  6:101370/1‑149 (MQ=255)
                                                            tgtgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCg                                                                          >  6:72704/1‑149 (MQ=255)
                                                              tgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTa                                                                        >  1:17703/1‑149 (MQ=255)
                                                               ggAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTAc                                                                       >  6:62186/1‑149 (MQ=255)
                                                                  aTGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGt                                                                    <  5:72704/149‑1 (MQ=255)
                                                                     tCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGttt                                                                  <  2:17703/148‑1 (MQ=255)
                                                                       aGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTaa                                                                <  5:101370/148‑1 (MQ=255)
                                                                           aTGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGC‑TGGACAACTTc                                                                                                                             <  8:32823/83‑1 (MQ=38)
                                                                                                  aCAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCg                                    >  8:80679/1‑149 (MQ=255)
                                                                                                    aaaCGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGtc                                  >  6:55650/1‑149 (MQ=255)
                                                                                                                   gTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGaaa                   <  5:62186/149‑1 (MQ=255)
                                                                                                                    ttAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTATAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTCGCGAAAc                  >  7:125537/1‑149 (MQ=255)
                                                                                                                     tAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTcc                                                     >  2:134203/1‑113 (MQ=255)
                                                                                                                     tAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACg                 >  3:44559/1‑149 (MQ=255)
                                                                                                                       aaaaCAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGcc               <  5:55650/149‑1 (MQ=255)
                                                                                                                       aaaaCAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGcc               >  7:119533/1‑149 (MQ=255)
                                                                                                                               aTCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAAt        >  5:67154/1‑148 (MQ=255)
                                                                                                                                  aGAAGTTGTCCATGCGC‑TGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctc                                                                                  >  4:99056/1‑71 (MQ=25)
                                                                                                                                  aGAAGTTGTCCATGCGC‑TGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctc                                                                                  <  3:99056/71‑1 (MQ=25)
                                                                                                                                  aGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAAc     <  6:67154/148‑1 (MQ=255)
                                                                                                                                   gAAGTTGTCCATGCGC‑TGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGt   >  1:193233/1‑149 (MQ=255)
                                                                                                                                    aaGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGAGTCTCTGTCCTGGCGAAACGCCCGACAATAACGtt  >  8:137767/1‑149 (MQ=255)
                                                                                                                                             a‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCtttt                                                                                            <  4:132373/50‑1 (MQ=255)
                                                                                                                                             a‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCtttt                                                                                            >  3:132373/1‑50 (MQ=255)
                                                                                                                                              |                                                                                                                                           
GAATTAATGCCGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTT  >  NC_000913/3183204‑3183484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑