| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,707,028 | 0 | T | G | 26.7% | 47.9 / 19.5 | 30 | V37G (GTA→GGA) | rsxC | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
| Reads supporting (aligned to +/- strand): ref base T (11/11); new base G (4/4); total (15/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCAC > NC_000913/1706886‑1707164 | ccATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTCTTCTCTGCATTCAGAAAAAATAATATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGTACCCCt < 6:85556/149‑1 (MQ=255) ccGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGTACCCCTGGCGCa > 2:83175/1‑149 (MQ=255) aTCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGTACCCCTGGCGCAGCGtttt < 3:140975/148‑1 (MQ=255) aTCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGTACCCCTGGCGCAGCGtttt > 4:140975/1‑148 (MQ=255) ccGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGGCGCCAGGTACCCCTGGCGCAGCGTTTTg > 2:79226/1‑143 (MQ=255) ccGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCAGCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGTACCCCTGGCGCAGCGTTTTg < 1:79226/142‑1 (MQ=255) aaGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGGGTACCTGGCGCAggggt > 8:58535/1‑117 (MQ=255) aaGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGGGTACCTGGCGCAggggt < 7:58535/121‑5 (MQ=255) tctGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGTACCCCTGGCGCAggggt > 6:70907/1‑108 (MQ=255) tctGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGTACCCCTGGCGCAggggt < 5:70907/112‑5 (MQ=255) cATTCAGAGAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGt < 1:50758/146‑1 (MQ=255) cATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGt < 1:83175/146‑1 (MQ=255) gAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGc < 3:132529/149‑1 (MQ=255) gAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGc > 4:132529/1‑149 (MQ=255) atCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGTACCCCTGGCGCAGCGTTTTGt < 5:75033/95‑1 (MQ=255) atCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGTACCCCTGGCGCAGCGTTTTGt > 6:75033/1‑95 (MQ=255) catccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGcc > 8:80242/1‑149 (MQ=255) ccGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGGGTACCTGGCGCAggg < 6:30138/59‑3 (MQ=21) ccGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGGGTACCTGGCGCAggg > 5:30138/1‑57 (MQ=21) gagaTGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGtt < 7:142105/101‑1 (MQ=255) gagaTGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGtt > 8:142105/1‑101 (MQ=255) gagaTGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCg > 5:81455/1‑112 (MQ=255) gagaTGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCg < 6:81455/112‑1 (MQ=255) gagaTGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTAccc > 2:113612/1‑148 (MQ=255) cGGTACACCCCT‑GCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAAtgctgc < 7:80242/149‑1 (MQ=255) cccT‑GCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCAc > 3:151661/1‑149 (MQ=255) gcT‑GCGCCAGGGGTACCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCt > 7:153879/2‑142 (MQ=255) gcT‑GCGCCAGGGGTACCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCt < 8:153879/141‑1 (MQ=255) t‑GCGCCAGGGGTACCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTgc > 4:83797/1‑99 (MQ=255) t‑GCGCCAGGGGTACCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTgc < 3:83797/99‑1 (MQ=255) | CCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCAC > NC_000913/1706886‑1707164 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |