| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,620,216 | 0 | C | T | 21.6% | 74.0 / 14.0 | 37 | intergenic (‑56/+30) | uraA/upp | uracil permease/uracil phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (16/13); new base T (5/3); total (21/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGT > NC_000913/2620107‑2620355 | gATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTa > 4:88599/1‑149 (MQ=255) gCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTa > 4:160347/1‑125 (MQ=255) tttCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTTTAAGAGTCGGCtttttt > 1:82372/1‑101 (MQ=39) tttCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTTTAAGAGTCGGCtttttt < 2:82372/104‑4 (MQ=39) acCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCttt > 7:68311/1‑110 (MQ=255) acCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCttt < 8:68311/110‑1 (MQ=255) cccGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTAtt < 6:7643/111‑1 (MQ=255) cccGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTAtt > 5:7643/1‑111 (MQ=255) cccGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGAtttt < 8:111803/127‑1 (MQ=255) cccGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGAtttt > 7:111803/1‑127 (MQ=255) gTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGt < 3:88599/149‑1 (MQ=255) tCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCgg > 8:162512/1‑124 (MQ=255) tCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCgg < 7:162512/124‑1 (MQ=255) tCCTCTGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTg > 2:61932/1‑100 (MQ=255) tCCTCTGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTg < 1:61932/100‑1 (MQ=255) ctctGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTccc > 8:67206/1‑149 (MQ=255) ttatGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGAtttt < 3:64658/90‑1 (MQ=255) ttatGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGAtttt > 4:64658/1‑90 (MQ=255) tatGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCt < 1:483/51‑1 (MQ=255) tatGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCt > 2:483/1‑51 (MQ=255) tatGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCg < 3:89315/126‑1 (MQ=255) tatGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCg > 4:89315/1‑126 (MQ=255) tgtTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGAtttt < 8:93711/85‑1 (MQ=255) tgtTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGAtttt > 7:93711/1‑85 (MQ=255) gtTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGAt > 5:25959/1‑148 (MQ=255) gtTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGAt < 6:25959/148‑1 (MQ=255) ttATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTAt < 4:12654/59‑1 (MQ=255) ttATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTAt > 3:12654/1‑59 (MQ=255) aaaGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCggg > 5:24462/1‑149 (MQ=255) aaGCCGACTTTAAGAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGt > 3:17133/1‑149 (MQ=255) aaGCCGACTTTAAGAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCAGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGt > 5:104179/1‑149 (MQ=255) aGCCGACTTTAAGAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCg < 2:168720/66‑1 (MQ=38) aGCCGACTTTAAGAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCg > 1:168720/1‑66 (MQ=38) aGCCGACTTTAAGAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGa > 2:90070/1‑139 (MQ=255) aGCCGACTTTAAGAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGa < 1:90070/139‑1 (MQ=255) ccGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGaataat < 6:100910/87‑1 (MQ=255) ccGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGaataat > 5:100910/1‑87 (MQ=255) | GATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGT > NC_000913/2620107‑2620355 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |