Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,620,2180TA21.6% 74.5 / 17.7 37intergenic (‑58/+28)uraA/uppuracil permease/uracil phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (16/13);  new base A (5/3);  total (21/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTT  >  NC_000913/2620107‑2620363
                                                                                                                |                                                                                                                                                 
gATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTa                                                                                                              >  4:88599/1‑149 (MQ=255)
                 gCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTa                                                                                                                     >  4:160347/1‑125 (MQ=255)
                       tttCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTTTAAGAGTCGGCtttttt                                                                                                                                    >  1:82372/1‑101 (MQ=39)
                       tttCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTTTAAGAGTCGGCtttttt                                                                                                                                    <  2:82372/104‑4 (MQ=39)
                               acCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCttt                                                                                                                      >  7:68311/1‑110 (MQ=255)
                               acCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCttt                                                                                                                      <  8:68311/110‑1 (MQ=255)
                                 cccGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTAtt                                                                                                                   <  6:7643/111‑1 (MQ=255)
                                 cccGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTAtt                                                                                                                   >  5:7643/1‑111 (MQ=255)
                                 cccGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGAtttt                                                                                                   <  8:111803/127‑1 (MQ=255)
                                 cccGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGAtttt                                                                                                   >  7:111803/1‑127 (MQ=255)
                                                 gTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGt                                                             <  3:88599/149‑1 (MQ=255)
                                                             tCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCgg                                                                           >  8:162512/1‑124 (MQ=255)
                                                             tCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCgg                                                                           <  7:162512/124‑1 (MQ=255)
                                                             tCCTCTGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTg                                                                                                  >  2:61932/1‑100 (MQ=255)
                                                             tCCTCTGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTg                                                                                                  <  1:61932/100‑1 (MQ=255)
                                                               ctctGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTccc                                               >  8:67206/1‑149 (MQ=255)
                                                                      ttatGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGAtttt                                                                                                   <  3:64658/90‑1 (MQ=255)
                                                                      ttatGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGAtttt                                                                                                   >  4:64658/1‑90 (MQ=255)
                                                                       tatGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCt                                                                                                                                         <  1:483/51‑1 (MQ=255)
                                                                       tatGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCt                                                                                                                                         >  2:483/1‑51 (MQ=255)
                                                                       tatGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCg                                                              >  4:89315/1‑126 (MQ=255)
                                                                       tatGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCg                                                              <  3:89315/126‑1 (MQ=255)
                                                                           tgtTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGAtttt                                                                                                   <  8:93711/85‑1 (MQ=255)
                                                                           tgtTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGAtttt                                                                                                   >  7:93711/1‑85 (MQ=255)
                                                                            gtTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGAt                                   >  5:25959/1‑148 (MQ=255)
                                                                            gtTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGAt                                   <  6:25959/148‑1 (MQ=255)
                                                                             ttATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTAt                                                                                                                           >  3:12654/1‑59 (MQ=255)
                                                                             ttATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTAt                                                                                                                           <  4:12654/59‑1 (MQ=255)
                                                                                                    aaaGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCggg           >  5:24462/1‑149 (MQ=255)
                                                                                                     aaGCCGACTTTAAGAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGt          >  3:17133/1‑149 (MQ=255)
                                                                                                     aaGCCGACTTTAAGAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCAGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGt          >  5:104179/1‑149 (MQ=255)
                                                                                                      aGCCGACTTTAAGAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCg                                                                                            <  2:168720/66‑1 (MQ=38)
                                                                                                      aGCCGACTTTAAGAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCg                                                                                            >  1:168720/1‑66 (MQ=38)
                                                                                                      aGCCGACTTTAAGAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGa                   >  2:90070/1‑139 (MQ=255)
                                                                                                      aGCCGACTTTAAGAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGa                   <  1:90070/139‑1 (MQ=255)
                                                                                                        ccGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGaataat                                                                     <  6:100910/87‑1 (MQ=255)
                                                                                                        ccGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGaataat                                                                     >  5:100910/1‑87 (MQ=255)
                                                                                                               ttAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTAt                                                                 >  3:10267/1‑84 (MQ=255)
                                                                                                               ttAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTAt                                                                 <  4:10267/84‑1 (MQ=255)
                                                                                                               ttAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCtttt  >  7:32243/1‑147 (MQ=255)
                                                                                                               ttAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCtttt  <  8:32243/147‑1 (MQ=255)
                                                                                                                |                                                                                                                                                 
GATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTC‑AAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTT  >  NC_000913/2620107‑2620363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑