Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,862,760	C→T	R142R (CGC→CGT)	ygbK →	FliA‑regulated DUF1537 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,862,760	0	C	T	100.0%	89.1 / NA	28	R142R (CGC→CGT)	ygbK	FliA‑regulated DUF1537 family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/14);  total (14/14)

CGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGC  >  NC_000913/2862631‑2862895                                                                                                                                  |                                                                                                                                        cGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCa                                                                                                                                       >  3:170357/1‑132 (MQ=255) cGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCa                                                                                                                                       <  4:170357/132‑1 (MQ=255)  gATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCAt                                                                                                                     <  6:12614/149‑1 (MQ=255)                                                    ccTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCt                                                                   >  5:168344/1‑149 (MQ=255)                                                                      tACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTc                                                      <  3:140129/144‑1 (MQ=255)                                                                      tACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTc                                                      >  4:140129/1‑144 (MQ=255)                                                                      tACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCg                                                     <  8:56799/145‑1 (MQ=255)                                                                      tACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCg                                                     >  7:56799/1‑145 (MQ=255)                                                                           tttATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGtt                                            <  6:128548/149‑1 (MQ=255)                                                                           tttATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGtt                                            >  5:128548/1‑149 (MQ=255)                                                                              aTCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGtttt                                          <  6:168344/148‑1 (MQ=255)                                                                                             tCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGAt                          >  8:99324/1‑149 (MQ=255)                                                                                               gTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGtttt                                          >  8:92303/1‑131 (MQ=255)                                                                                                    gAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTc                   >  4:71526/1‑149 (MQ=255)                                                                                                         aaCTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGc                        <  4:136751/139‑1 (MQ=255)                                                                                                         aaCTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGc                        >  3:136751/1‑139 (MQ=255)                                                                                                            tgctgGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTc                   >  4:121966/1‑141 (MQ=255)                                                                                                            tgctgGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTc                   <  3:121966/141‑1 (MQ=255)                                                                                                              ctgGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCt         <  7:99324/149‑1 (MQ=255)                                                                                                               tgGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCaa              <  4:28435/143‑1 (MQ=255)                                                                                                               tgGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCaa              >  3:28435/1‑143 (MQ=255)                                                                                                                 gCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAg                                  <  7:100295/121‑1 (MQ=255)                                                                                                                 gCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAg                                  >  8:100295/1‑121 (MQ=255)                                                                                                                     aaTCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGc  <  3:71526/149‑1 (MQ=255)                                                                                                                       tCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTACCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGc  <  5:52282/147‑1 (MQ=255)                                                                                                                       tCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTACCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGc  >  6:52282/1‑147 (MQ=255)                                                                                                                          gggATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGCAGCGCACTCCACAGGGCGCTGCGGCGTCGGGTCGGCACAGGTGTTCGAACAAGGGGTGGCTGCCGTTc                   >  3:34440/1‑127 (MQ=255)                                                                                                                          gggAGGCGTCATCACCCGGTAACTCCCATGACCTCCAGCTATCTTCCCCGTCTGGTGGAAGCGCAATCCACAGGGCGCTGCGGCGGCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTc                   <  4:34440/127‑1 (MQ=255)                                                                                                                                  |                                                                                                                                        CGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGC  >  NC_000913/2862631‑2862895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑