| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,540,647 | 0 | G | C | 20.0% | 57.0 / 10.3 | 25 | R648R (CGC→CGG) | narZ | nitrate reductase 2 (NRZ), alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (9/11); new base C (2/3); total (11/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTCGCAGGCCATACGTAAATCGCCCGATTTCAGCGCCTGGGCGGTAAATTCTGTGGGGGATAATCCGGCCTTGTCGGCTTCAGCTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACTCCTGAGCAGAGACTTTTTCATAGCGCCATTGGCTGGAATGATTGTAGAAAAACGAGGTGCTGTTCATTTGGCGCGG > NC_000913/1540499‑1540787 | gttCGCAGGCCATACGTAAATCGCCCGATTTCAGCGCCTGGGCGGTAAATTCTGTGGGGGATAATCCGGCCTTGTCGGCTTCAGCTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGgcg > 2:93075/1‑149 (MQ=255) ttCGCAGGCCATACGTAAATCGCCCGATTTCAGCGCCTGGGCGGTAAATTCTGTGGGGGATAATCCGGCCTTGTCGGCTTCAGCTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGgcgc > 2:148202/1‑149 (MQ=255) cAGGCCATACGTAAATCGCCCGATTTCAGCGCCTGGGCGGTAAATTCTGTGGGGGATAATCCGGCCTTGTCGGCTTCAGCTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAAc < 1:148202/149‑1 (MQ=255) aTACGTAAATCGCCCGATTTCAGCGCCTGGGCGGTAAATTCTGTGGGGGATAATCCGGCCTTGTCGGCTTCAGCTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGCGCCGAACGtatgg < 4:67644/149‑5 (MQ=255) gCCCGATTTCAGCGCCTGGGCGGTAAATTCTGTGGGGGATAATCCGGCCTTGTCGGCTTCAGCTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGAt < 8:62140/149‑1 (MQ=255) gATTTCAGCGCCTGGGCGGTAAATTCTGTGGGGGATAATCCGGCCTTGTCGGCTTCAGCTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGAcc < 1:93075/149‑1 (MQ=255) gggATAATCCGGCCTTGTCGGCTTCAGCTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCgg < 1:135815/145‑1 (MQ=255) gggATAATCCGGCCTTGTCGGCTTCAGCTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCgg > 2:135815/1‑145 (MQ=255) ggATAATCCGGCCTTGTCGGCTTCAGCTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGa > 3:47780/1‑147 (MQ=255) tCCGGCCTTGTCGGCTTCAGCTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACt > 7:78033/1‑149 (MQ=255) cGGCCTTGTCGGCTTCAGCTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGc < 2:120445/111‑1 (MQ=255) cGGCCTTGTCGGCTTCAGCTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGc > 1:120445/1‑111 (MQ=255) ggCCTTGTCGGCTTCAGCTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACTCCt < 4:47780/149‑1 (MQ=255) cTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACTCCTGAGCAGAGACTTTTTCa < 8:78033/149‑1 (MQ=255) ccGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGta < 2:65900/89‑1 (MQ=255) ccGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGta > 1:65900/1‑89 (MQ=255) gCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACTCCTGAGCAGAGACTTTTTCATAGCGCCATTGGCTGGAATGa > 6:3109/1‑149 (MQ=255) ccccAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACTCCTGAGCAGAGACTTTTTCATAGCGCCATTGGCTGGAATGAt < 5:3109/149‑1 (MQ=255) ccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACTCCTGAGCAGAGACTTTTTCATAGCGCCATTGGCTGGAATGATTGTAGAAAAACGAGGTGCTGTTCa > 2:122317/1‑149 (MQ=255) cccATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACTCCTGAGCAGAGACTTTTTCATAGCGCCATTGGCTGGAATGATTGTAGAAAAACGAGGTGCTGTTCATTTg > 2:140526/1‑149 (MQ=255) caaCGTTCGCGCCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACTCCTGAGCAGAGACTTTTTCATAGCGCCATTGGCTGGAATGATTGTAGAAAAACGAGGTGCTGTTCAttt < 7:943/143‑1 (MQ=255) caaCGTTCGCGCCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACTCCTGAGCAGAGACTTTTTCATAGCGCCATTGGCTGGAATGATTGTAGAAAAACGAGGTGCTGTTCAttt > 8:943/3‑145 (MQ=255) caaCGTTCGCGCCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACTCCTGAGCAGAGACTTTTTCATAGCGCCATTGGCTGGAATGATTGTAGAAAAACGAGGTGCTGTTCAttt < 6:76359/143‑1 (MQ=255) caaCGTTCGCGCCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACTCCTGAGCAGAGACTTTTTCATAGCGCCATTGGCTGGAATGATTGTAGAAAAACGAGGTGCTGTTCAttt > 5:76359/3‑145 (MQ=255) taagTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACTCCTGAGCAGAGACTTTTTCATAGCGCCATTGGCTGGAATGATTGTAGAAAAACGAGGTGCTGTTCAttt > 4:135012/4‑144 (MQ=255) tACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACTCCTGAGCAGAGACTTTTTCATAGCGCCATTGGCTGGAATGATTGTAGAAAAACGAGGTGCTGTTCAttt < 3:135012/144‑1 (MQ=255) cGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACTCCTGAGCAGAGACTTTTTCATAGCGCCATTGGCTGGAATGATTGTAGAAAAACGAGGTGCTGTTCATTTGGCGCgg < 1:140526/149‑1 (MQ=255) | GTTCGCAGGCCATACGTAAATCGCCCGATTTCAGCGCCTGGGCGGTAAATTCTGTGGGGGATAATCCGGCCTTGTCGGCTTCAGCTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACTCCTGAGCAGAGACTTTTTCATAGCGCCATTGGCTGGAATGATTGTAGAAAAACGAGGTGCTGTTCATTTGGCGCGG > NC_000913/1540499‑1540787 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |