Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	757,084	C→T	S393L (TCG→TTG)	sdhA →	succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	757,084	0	C	T	100.0%	145.2 / NA	42	S393L (TCG→TTG)	sdhA	succinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/21);  total (21/21)

GAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTC  >  NC_000913/756939‑757227                                                                                                                                                  |                                                                                                                                                gagCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTgg                                                                                                                                               >  6:76812/1‑148 (MQ=255)     cGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTGGTacac                                                                                                                                          <  6:19298/149‑1 (MQ=255)           cGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTGGt                                                                                                                                              <  1:99678/139‑1 (MQ=255)           cGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTGGt                                                                                                                                              >  2:99678/1‑139 (MQ=255)               tATCCCCACCTGTCACTACCTGCTTGGCCGTATTCCGACCAAATGTACCGGTCATGCACTGCATTTGTATGCTAAATGCGAATCTGTGGTTGGTCCGGGAATGGTTGCCGTTGGTGAAATCGCTTGTGTATTGGTACACGGCGCTAAcc                                                                                                                                <  8:105149/149‑1 (MQ=255)                 tCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTGGTACACGGCGCTAACCGt                                                                                                                              <  5:76812/149‑1 (MQ=255)                  cccAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTGGt                                                                                                                                              <  8:120648/132‑1 (MQ=255)                  cccAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTGGt                                                                                                                                              >  7:120648/1‑132 (MQ=255)                   ccAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTGGTACACGGCGCTAACCGTCt                                                                                                                            >  2:198803/1‑149 (MQ=255)                     aaCCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTGGTACACGGCGCTAACCGTCTgg                                                                                                                          >  6:70154/1‑149 (MQ=255)                              cTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTGGTACACGGCGCTAACCGTCTGGGCGGCAACt                                                                                                                 >  7:162500/1‑149 (MQ=255)                               tACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTGGTacac                                                                                                                                          <  1:193248/123‑1 (MQ=255)                               tACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTGGTacac                                                                                                                                          >  2:193248/1‑123 (MQ=255)                                         gCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGAc                                                                                                      >  1:96707/1‑149 (MQ=255)                                            gTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCtg                                                                                                   <  6:172653/149‑1 (MQ=255)                                             tATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTGGTACACGGCGCTAACCGTCTGGGCGGCAACTCgct                                                                                                             >  4:123558/1‑138 (MQ=255)                                             tATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTGGTACACGGCGCTAACCGTCTGGGCGGCAACTCgct                                                                                                             <  3:123558/138‑1 (MQ=255)                                               ttCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCtggtg                                                                                                <  1:198803/149‑1 (MQ=255)                                                    aCCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCtt                                                                                           <  2:96707/149‑1 (MQ=255)                                                      cAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTg                                                                                         <  8:162500/149‑1 (MQ=255)                                                              ccGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTcgcgc                                                                                  <  5:70154/148‑1 (MQ=255)                                                              ccGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTcgcgc                                                                                  <  5:81543/148‑1 (MQ=255)                                                                 gTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCtg                                                                                                   >  6:19279/1‑128 (MQ=255)                                                                          tgactgTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCttt                                                                                          <  2:3544/128‑1 (MQ=255)                                                                          tgactgTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCttt                                                                                          >  1:3544/1‑128 (MQ=255)                                                                            actgTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCGTGTGTATTGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGtctgcatct                                                                   <  5:71967/149‑1 (MQ=255)                                                                               gtgAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTgg                                                                                                                                               >  7:118232/1‑70 (MQ=255)                                                                               gtgAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTgg                                                                                                                                               <  8:118232/70‑1 (MQ=255)                                                                                              gAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGcc                                                   >  4:145879/1‑147 (MQ=255)                                                                                                    gtgGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAggg                                           >  5:131255/1‑149 (MQ=255)                                                                                                    gtgGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAggg                                           >  7:156430/1‑149 (MQ=255)                                                                                                       gttgttCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGtctgca                                                                      <  4:40602/119‑1 (MQ=255)                                                                                                       gttgttCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGtctgca                                                                      >  3:40602/1‑119 (MQ=255)                                                                                                          gttCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACt                                     >  2:83829/1‑149 (MQ=255)                                                                                                           ttCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAg                                                                             >  6:167867/1‑108 (MQ=255)                                                                                                           ttCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATTGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAg                                                                             <  5:167867/108‑1 (MQ=255)                                                                                                                          ccGTTGGTGAAATCGCTTGTGTATTGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCtggtgg                                                                                               <  6:176873/75‑1 (MQ=255)                                                                                                                          ccGTTGGTGAAATCGCTTGTGTATTGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCtggtgg                                                                                               >  5:176873/1‑75 (MQ=255)                                                                                                                          ccGTTGGTGAAATCGCTTGTGTATTGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGa                      <  6:131255/148‑1 (MQ=255)                                                                                                                            gTTGGTGAAATCGCTTGTGTATTGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTc                   >  8:40755/1‑149 (MQ=255)                                                                                                                                 tGAAATCGCTTGTGTATTGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATg              <  1:83829/149‑1 (MQ=255)                                                                                                                                             tgtATTGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGtctc  >  7:47999/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                GAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTC  >  NC_000913/756939‑757227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑