Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,683,473 G→A intergenic (‑26/+157) dctA ← / ← yhjK C4‑dicarboxylic acid, orotate and citrate transporter/cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,683,4730GA97.5% 527.1 / 0.2 159intergenic (‑26/+157)dctA/yhjKC4‑dicarboxylic acid, orotate and citrate transporter/cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base A (83/70);  total (87/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 159 total aligned reads displayed.

AGCCGTCGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAA  >  NC_000913/3683327‑3683615
                                                                                                                                                  |                                                                                                                                              
aGCCGTCGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTCCATAGGGTGTCCTTTAGTAAAACCACAGATc                                                                                                                                              <  1:113684/149‑1 (MQ=255)
  ccGTCGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGCGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCtt                                                                                                                                            <  1:78621/149‑1 (MQ=255)
   cGTCGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTg                                                                                                                                           <  7:81078/149‑1 (MQ=255)
   cGTCGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTg                                                                                                                                           >  3:99930/1‑149 (MQ=255)
     tCGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGt                                                                                                                                          >  2:403358/1‑148 (MQ=255)
       gCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTaa                                                                                                                                        <  1:24380/148‑1 (MQ=255)
       gCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAg                                                                                                                                       <  5:298760/149‑1 (MQ=255)
        ccAAGCTGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAgg                                                                                                                                      >  8:164593/1‑149 (MQ=255)
        ccAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATTGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAgg                                                                                                                                      <  5:21989/149‑1 (MQ=255)
          aaGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGt                                                                                                                                     >  7:254453/1‑148 (MQ=255)
            gCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTAt                                                                                                                                  <  5:398649/149‑1 (MQ=255)
             cGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATg                                                                                                                                 <  6:339384/149‑1 (MQ=255)
             cGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATg                                                                                                                                 <  6:36093/149‑1 (MQ=255)
                    aTTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTATCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTAcc                                                                                                                          >  6:121506/1‑149 (MQ=255)
                       tGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGc                                                                                                                       >  6:129750/1‑149 (MQ=255)
                          tCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGc                                                                                                                    >  1:301737/1‑149 (MQ=255)
                          tCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGc                                                                                                                    >  1:69489/1‑149 (MQ=255)
                          tCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGc                                                                                                                    >  1:377834/1‑149 (MQ=255)
                               tATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAATCAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTa                                                                                                                >  6:195565/1‑148 (MQ=255)
                                 tttCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTaaa                                                                                                              <  2:36203/148‑1 (MQ=255)
                                   tCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTAAAAAt                                                                                                            <  3:196347/148‑1 (MQ=255)
                                     aGGATAGAAATGGCCAAGGAGAATACCAATGTCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTCTGGGGTACCTGCGGCCTTAAAATAac                                                                                                         <  5:165368/149‑1 (MQ=255)
                                          aGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACAcc                                                                                                      >  1:70027/1‑147 (MQ=255)
                                                 gCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGacaac                                                                                              <  1:326260/148‑1 (MQ=255)
                                                  ccAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGac                                                                                                 >  7:160040/1‑144 (MQ=255)
                                                   cAAGGAGAATACCAATGGCTATCGCTGTCAGGCCCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACAt                                                                                            >  2:265287/1‑148 (MQ=255)
                                                   cAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACATGCGGCCTTAAAATAACACCCAGACAACAt                                                                                            >  4:117923/1‑148 (MQ=255)
                                                      ggAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATCACACCCAGACAACATcaca                                                                                        >  2:232898/1‑149 (MQ=255)
                                                      ggAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTCAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGc                                                                                                                    <  4:113436/121‑1 (MQ=255)
                                                       gagaATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAg                                                                                       <  4:168415/149‑1 (MQ=255)
                                                         gaATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGCTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGa                                                                                      >  8:66323/1‑148 (MQ=255)
                                                          aaTACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGAAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGc                                                                                                                    <  2:63423/117‑1 (MQ=255)
                                                           aTACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAAt                                                                                   <  7:58018/149‑1 (MQ=255)
                                                            tACCTATGGCTATCTCTGGCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTATCCTTTAGTAAACCCACAGATCTTGTAAGGGTATGGGTTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATg                                                                                  <  1:384492/149‑1 (MQ=255)
                                                            tACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCa                                                                                                    <  6:199318/131‑1 (MQ=255)
                                                            tACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGac                                                                                                 >  8:402698/1‑134 (MQ=255)
                                                            tACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATg                                                                                  >  7:92260/1‑149 (MQ=255)
                                                            tACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATg                                                                                  >  8:181121/1‑149 (MQ=255)
                                                             aCCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAacac                                                                                                       >  7:198641/1‑127 (MQ=255)
                                                             aCCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAacac                                                                                                       <  8:198641/127‑1 (MQ=255)
                                                              ccAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTa                                                                                <  8:329657/149‑1 (MQ=255)
                                                               cAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTAc                                                                               >  7:112223/1‑149 (MQ=255)
                                                               cAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTAc                                                                               >  8:222316/1‑149 (MQ=255)
                                                                 aTGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCt                                                                             <  6:391145/149‑1 (MQ=255)
                                                                 aTGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCt                                                                             <  4:99930/149‑1 (MQ=255)
                                                                    gCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGa                                                                          <  2:377834/149‑1 (MQ=255)
                                                                     cTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGAt                                                                         <  3:324524/149‑1 (MQ=255)
                                                                        tCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCa                                                                       <  2:69489/148‑1 (MQ=255)
                                                                          gCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATaa                                                                    >  5:287128/1‑149 (MQ=255)
                                                                          gCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATaa                                                                    <  1:358501/149‑1 (MQ=255)
                                                                              tCATGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAAtt                                                                  <  5:218952/147‑1 (MQ=255)
                                                                              tCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTg                                                                 >  7:186936/1‑148 (MQ=255)
                                                                                aGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGaa                                                               <  6:380990/148‑1 (MQ=255)
                                                                                  gACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACATTCGGCCTTAAAATAACACCCAGACAACATCAAAGAAATCTACCTGGAGAAAAATTGAAAc                                                             >  8:151599/1‑148 (MQ=255)
                                                                                     cTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACaaaa                                                         <  3:117923/149‑1 (MQ=255)
                                                                                     cTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACaaa                                                          >  6:152720/1‑148 (MQ=255)
                                                                                       gAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAg                                                       >  8:132571/1‑149 (MQ=255)
                                                                                       gAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAg                                                       >  6:392437/1‑149 (MQ=255)
                                                                                        aaaGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAg                                                       <  3:224753/148‑1 (MQ=255)
                                                                                            tAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTa                                                   >  7:278650/1‑148 (MQ=255)
                                                                                             aaaGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTATGGTTATGGGGTACGTGCGGCCTTAAGATAACACCCAGACACCATGACTGAAATGTCCCTGGAGCTTAATTGAAACAAAAAGGttta                                                  >  2:331593/1‑146 (MQ=255)
                                                                                              aaGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAAc                                                             <  8:257236/136‑1 (MQ=255)
                                                                                              aaGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAAc                                                             >  7:257236/1‑136 (MQ=255)
                                                                                              aaGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTaaa                                                 >  5:147369/1‑148 (MQ=255)
                                                                                                 tcTTTTAAACAGAGAGGTTTTCATAGGGTGGCCTTTAGTAAAACAACAGATCTTGTAAGGTTATGGTGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGt                                             <  5:121506/148‑1 (MQ=255)
                                                                                                  cTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGtt                                            <  4:178313/149‑1 (MQ=255)
                                                                                                   ttttAAACAGAGAGGTTTTCATAGTGTGTCCTTTATTAAAACCACAGATCTTGTAATTTTATTTGGTACCTTCTGCATTAAAATAAAACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGttt                                           <  7:423646/149‑1 (MQ=255)
                                                                                                     ttAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGttt                                           <  8:248389/147‑1 (MQ=255)
                                                                                                      tAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGa                                         <  8:112223/148‑1 (MQ=255)
                                                                                                       aaaCAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCCCAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTATCAGc                                       >  2:108097/1‑149 (MQ=255)
                                                                                                        aaCAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTa                                                   >  8:305492/1‑136 (MQ=255)
                                                                                                           agagagGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGc                                   >  8:265457/1‑149 (MQ=255)
                                                                                                             agagGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTc                                 >  3:44833/1‑149 (MQ=255)
                                                                                                                  ttttCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACATGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGcac                             >  1:222876/1‑148 (MQ=255)
                                                                                                                      cATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAAc                         <  5:339479/148‑1 (MQ=255)
                                                                                                                       aTAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACaacc                         >  4:249801/1‑145 (MQ=255)
                                                                                                                       aTAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAAc                         >  4:39536/1‑147 (MQ=255)
                                                                                                                       aTAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAAc                         >  1:158237/1‑147 (MQ=255)
                                                                                                                        tAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAg                      <  1:232898/149‑1 (MQ=255)
                                                                                                                        tAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAg                      <  7:108162/149‑1 (MQ=255)
                                                                                                                           ggTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCAc                   >  7:3786/1‑149 (MQ=255)
                                                                                                                           ggTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCAc                   >  4:301541/1‑149 (MQ=255)
                                                                                                                            gtgtCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGCTTGAGCTGGCTCGCACAAACCAGCACt                  >  8:41819/1‑149 (MQ=255)
                                                                                                                             tgtCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACt                  <  2:241200/148‑1 (MQ=255)
                                                                                                                               tCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACtttt               <  5:415037/149‑1 (MQ=255)
                                                                                                                               tCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACttt                <  8:333479/148‑1 (MQ=255)
                                                                                                                               tCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAAGGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTTGCTCGCACAAACCAGCACtttt               >  6:54410/1‑149 (MQ=255)
                                                                                                                                 cTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTa             <  5:39939/149‑1 (MQ=255)
                                                                                                                                    tAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTg                                                                            <  6:42981/83‑1 (MQ=255)
                                                                                                                                    tAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTg                                                                            >  5:42981/1‑83 (MQ=255)
                                                                                                                                      gTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGATAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGtt        >  7:305677/1‑149 (MQ=255)
                                                                                                                                       tAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGttt       >  3:109366/1‑149 (MQ=255)
                                                                                                                                         aaaCCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGtttt      <  5:195565/148‑1 (MQ=255)
                                                                                                                                         aaaCCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGtttt      <  1:245885/148‑1 (MQ=255)
                                                                                                                                         aaaCCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGtttt      <  6:147369/148‑1 (MQ=255)
                                                                                                                                         aaaCCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGtttt      <  6:170258/148‑1 (MQ=255)
                                                                                                                                         aaaCCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGtttt      <  6:407499/148‑1 (MQ=255)
                                                                                                                                         aaaCCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTg     <  3:102545/149‑1 (MQ=255)
                                                                                                                                             cacaGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGtttt      <  1:423120/144‑1 (MQ=255)
                                                                                                                                             cacaGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTaa  >  2:227110/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
AGCCGTCGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAA  >  NC_000913/3683327‑3683615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: