New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 781222	50 (1.470)	7 (0.210) +ATGT	7/252	2.5	12.6%	noncoding (76/76 nt)	lysY	tRNA‑Lys
	?	NC_000913	= 781839	NA (NA)					intergenic (+187/‑246)	lysQ/nadA	tRNA‑Lys/quinolinate synthase, subunit A
Rejected: Frequency below/above cutoff threshold.

GGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTA  >  NC_000913/781086‑781367                                                                                                                                          |                                                                                                                                                  ggAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCa                                                                                                                                       <  7:85393/149‑1 (MQ=35)       cACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCa                                                                                                                                 <  7:112024/149‑1 (MQ=40)           tCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATg                                                                                                                             >  1:124929/1‑149 (MQ=255)               ttACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAAc                                                                                                                                           >  4:108153/1‑131 (MQ=34)               ttACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAAc                                                                                                                                           <  3:108153/131‑1 (MQ=34)                           ggTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGAt                                                                                                              >  4:75855/1‑148 (MQ=255)                             tCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGaa                                                                                                                  <  1:101634/142‑1 (MQ=255)                             tCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGaa                                                                                                                  >  2:101634/1‑142 (MQ=255)                                        aTCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAg                                                                                                 >  6:40461/1‑148 (MQ=255)                                             gTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACgg                                                                                           <  3:75855/149‑1 (MQ=255)                                              tcatcgCCCACCAC‑ACGGGGCGTTAGCTCAGTTGGTTGAGCAGTTGGCTGTTTATCAATTGGTCGCATGTTGGAATCCTGCACGACCCACCAGTTTTAACAGCAAACTCAGAGGTTTAGCGTGAAGGATGACGTTGAGCCCGCCCCGGc                                                                                          >  4:45161/1‑149 (MQ=14)                                               catcaCCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGa                                                                                                                   <  6:24212/123‑1 (MQ=255)                                               catcaCCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGa                                                                                                                   >  5:24212/1‑123 (MQ=255)                                                atcaCCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGccc                                                                                        >  5:81958/1‑149 (MQ=255)                                                     ccaccaCTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAatgtaaaaaagcgcctttagggcgcttttttacattggtgagtc                                                                                                       >  6:42821‑M1/1‑86 (MQ=255)                                                     ccaccaCTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAatgtaaaaaagcgcctttagggcgcttttttacattggtgagtc                                                                                                       <  5:42821‑M1/130‑45 (MQ=255)                                                     ccaccaC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACAt                                                                                                                                         >  7:84815/1‑95 (MQ=37)                                                     ccaccaC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACAt                                                                                                                                         <  8:84815/95‑1 (MQ=37)                                                             tCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGgcgagc                                                                             >  4:99273/1‑148 (MQ=255)                                                                  tCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGagcgaagc                                                                        >  6:28154/1‑148 (MQ=255)                                                                  tCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGagcgaagc                                                                        >  6:21422/1‑148 (MQ=255)                                                                    gTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAg                                                                     <  5:21422/149‑1 (MQ=255)                                                                        gCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGgcga                                                                               <  1:107939/135‑1 (MQ=255)                                                                        gCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGgcga                                                                               >  2:107939/1‑135 (MQ=255)                                                                         cTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATc                                                                <  5:28154/149‑1 (MQ=255)                                                                            aGTTGGTAGCGCAGGTGACTTTTAATCAATTGGGCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCt                                                              <  3:45161/148‑1 (MQ=255)                                                                            aGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATcc                                                               <  5:40461/147‑1 (MQ=255)                                                                             gTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGc                                                            <  2:124929/149‑1 (MQ=255)                                                                                   agagCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGACGCGAGTCATCCTGCACGAcc                                                      >  8:114905/1‑149 (MQ=255)                                                                                          ttGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGagcgaagcga                                                                      <  8:19627/126‑1 (MQ=255)                                                                                          ttGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGagcgaagcga                                                                      >  7:19627/1‑126 (MQ=255)                                                                                             aCTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCt                                             >  5:104523/1‑148 (MQ=255)                                                                                               ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAatgtaaaaaagcgcctttagggcgcttttttacattggt                                                                                                            <  2:138481‑M1/83‑40 (MQ=255)                                                                                               ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAatgtaaaaaagcgcctttagggcgcttttttacattggt                                                                                                            >  1:138481‑M1/1‑44 (MQ=255)                                                                                                tttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTa                                           >  6:51009/1‑147 (MQ=255)                                                                                                 ttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCttta                                          <  2:158818/147‑3 (MQ=255)                                                                                                    aTCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGagcgaagc                                                                        >  8:77332/1‑114 (MQ=255)                                                                                                    aTCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGagcgaagc                                                                        <  7:77332/114‑1 (MQ=255)                                                                                                       aaTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAg                                                                                    >  2:69963/1‑99 (MQ=255)                                                                                                       aaTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAg                                                                                    <  1:69963/99‑1 (MQ=255)                                                                                                       aaTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGc                                  <  5:51009/149‑1 (MQ=255)                                                                                                            gTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGa                             <  6:104523/149‑1 (MQ=255)                                                                                                             tCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAg                            >  7:13257/1‑149 (MQ=255)                                                                                                               gCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAgg                                                                                   >  6:37349/1‑92 (MQ=255)                                                                                                               gCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAgg                                                                                   <  5:37349/92‑1 (MQ=255)                                                                                                                 aGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGa                             <  4:33752/144‑1 (MQ=255)                                                                                                                 aGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGa                             >  3:33752/1‑144 (MQ=255)                                                                                                                        aaTCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCttt                 <  7:114905/149‑1 (MQ=255)                                                                                                                        aaTCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCttt                 <  6:81958/149‑1 (MQ=255)                                                                                                                         aTCCTGCACGACCCACCAatgtaaaaaagcgccctaaaggcgcttttttacattggtgggtcgtgcaggatgactcgcttcgctcgcccttcgggccgtcgccgcgagcggctccgttgtttcgctgcgctcaactcaaacctgctg                  >  3:113207‑M1/1‑18 (MQ=255)                                                                                                                                     ccaccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCg    <  3:99273/149‑1 (MQ=255)                                                                                                                                      caccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCg    >  4:103488/1‑148 (MQ=255)                                                                                                                                       accaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTa  >  1:39794/1‑149 (MQ=255)                                                                                                                                        ccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTaaa                                         >  4:88577/1‑109 (MQ=255)                                                                                                                                        ccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTaaa                                         <  3:88577/109‑1 (MQ=255)                                                                                                                                          aatgtaaaaaagcgccctaaaggcgcttttttgcattggtgggtcgtgcaggatgactcgcttcgctcgcccttcgggccgtcgccgcgagcggctccgttgtttcgctgcgctcaactcaaacctgctgcaggttcgaatct     >  7:56956‑M1/1‑1 (MQ=255)                                                                                                                                          aatgtaaaaaagcgccctaaaggcgcttttttgcattggtgggtcgtgcaggatgactcgcttcgctcgcccttcgggccgtcgccgcgagcggctccgttgtttcgctgcgctcaactcaaacctgctgcaggttcgaatct     <  8:56956‑M1/143‑143 (MQ=255)                                                                                                                                          |                                                                                                                                                  GGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTA  >  NC_000913/781086‑781367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.