Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,362,096	Δ1 bp	coding (186/558 nt)	puuR →	repressor for the divergent puu operons, putrescine inducible

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,362,096	0	A	.	100.0%	239.7 / NA	53	coding (186/558 nt)	puuR	repressor for the divergent puu operons, putrescine inducible
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (30/23);  total (30/23)

CGCCAGCAGCAGGGGCTTTCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTCACTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTGAGATGGGTAGTCAGGGTGTGTCAATGAAGCTGGTTCATAACGGTAACCCGAATCGCACGCTGGCGATGAT  >  NC_000913/1361956‑1362239                                                                                                                                             |                                                                                                                                                cGCCAGCAGCAGGGGCTTTCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGGCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGaa                                                                                                                                        <  4:273637/149‑1 (MQ=255)           agGGGCTTTCAGAACGTCGTGCCGCCGAAGTCTCCGGGCTGACGCTCAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGTACTCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCg                                                                                                                              <  5:155581/149‑1 (MQ=255)               gCTTTCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGcc                                                                                                                          >  4:276367/1‑149 (MQ=255)                 tttCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCg                                                                                                                         >  5:149252/1‑148 (MQ=255)                     acaACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGCTAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGaaa                                                                                                                     >  6:232967/1‑148 (MQ=255)                     acaACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGTACGCTGCAACAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGaaaa                                                                                                                    >  2:144975/1‑149 (MQ=255)                                  gccgAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCg                                                                                                       >  2:87663/1‑149 (MQ=255)                                   ccgAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCg                                                                                                       >  2:218492/1‑148 (MQ=255)                                     gAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGTCCGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAg                                                                                                    <  6:252081/149‑1 (MQ=255)                                     gAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAAt                                                                                                                                       <  1:185469/114‑1 (MQ=255)                                     gAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAAt                                                                                                                                       >  2:185469/1‑114 (MQ=255)                                           tcCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGgtcgt                                                                                               >  6:316709/1‑148 (MQ=255)                                                gCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGaaaaa                                                                                                                   <  1:177633/123‑1 (MQ=255)                                                gCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGaaaaa                                                                                                                   >  2:177633/1‑123 (MQ=255)                                                    aCTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATc                                                                                       <  1:59011/147‑1 (MQ=255)                                                    aCTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAg                                                                                     <  4:144859/149‑1 (MQ=255)                                                      tCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGga                                                                                   >  3:40040/1‑149 (MQ=255)                                                      tCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGga                                                                                   >  3:213870/1‑149 (MQ=255)                                                        acaGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGgac                                                                                  >  7:259340/1‑148 (MQ=255)                                                               tATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAAt                                                                          >  3:232511/1‑149 (MQ=255)                                                                   aGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTga                                                                       <  8:274094/148‑1 (MQ=255)                                                                    gTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTgaga                                                                     <  3:276367/149‑1 (MQ=255)                                                                    gTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTgaga                                                                     <  4:40040/149‑1 (MQ=255)                                                                       cGATAGAACAAGATAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTGAGATgg                                                                  <  8:259340/149‑1 (MQ=255)                                                                        gATAGAACAAGATAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCtttt                                                                                                                                 >  1:277146/1‑85 (MQ=255)                                                                        gATAGAACAAGATAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGATGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCtttt                                                                                                                                 <  2:277146/85‑1 (MQ=255)                                                                             aaCAAGATAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTGAGATGGGTAGt                                                             >  2:131655/1‑148 (MQ=255)                                                                               cAAGATAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTGAGATGGGTAGTCAg                                                          >  8:19906/1‑149 (MQ=255)                                                                                  gATAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGCGCCGCAGGTCGTCATTAATCAGGACGACTTAATTGAGATGGGTAGTCAGGgt                                                       >  5:189721/1‑149 (MQ=255)                                                                                  gATAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTGAGATGGGTAGTCAGGgt                                                       <  4:232511/149‑1 (MQ=255)                                                                                   aTAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTGAGATggg                                                                 <  2:235755/138‑1 (MQ=255)                                                                                   aTAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTGAGATggg                                                                 >  1:235755/1‑138 (MQ=255)                                                                                     aaaGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTGAGATGGGTAGTCAGGgtgtg                                                    >  7:161211/1‑149 (MQ=255)                                                                                       aGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTGAGATGGGTAGTCAGGgtgtgt                                                   >  2:38432/1‑148 (MQ=255)                                                                                        gTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTGAGATGGGTAGTCAGGGTGTGTc                                                  >  8:177002/1‑148 (MQ=255)                                                                                            gCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTGAGATGGGTAGTCAGGGTGTGTCAATg                                              >  5:152354/1‑148 (MQ=255)                                                                                             cccTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTGAGATGGGTAGTCAGGGTGTGTCAATg                                              >  1:27220/1‑147 (MQ=255)                                                                                               cTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTGAGATGGGTAGTCAGGGTGTGTCAATGAAGc                                          <  5:232967/149‑1 (MQ=255)                                                                                                    aTCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTGAGATGGGTAGTCAGGGTGTGTCAATGAAGCTGGtt                                     <  6:149252/149‑1 (MQ=255)                                                                                                          aCGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTGAGATGGGTAGTCAGGGTGTGTCAATGAAGCTGGTTCATAAc                               >  7:274185/1‑149 (MQ=255)                                                                                                            gCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTGAGATgg                                                                  >  3:16585/1‑112 (MQ=255)                                                                                                            gCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTGAGATgg                                                                  <  4:16585/112‑1 (MQ=255)                                                                                                                cAAAAGCTGCTGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTGAGATGGGTAGTCAGGGTGTGTCAATGAAGCTGGTTCATAACGGTAAc                         >  1:226459/1‑149 (MQ=255)                                                                                                                     gctgctGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTGAGATGGGTAGTCAGGGTGTGTCAATGAAGCTGGTTCATAACGGTAACCCGaa                    <  8:161211/149‑1 (MQ=255)                                                                                                                     gctgctGAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATAAGGACGACTTAATTGAGATGGGTAGTCAGGGTGTGTCAATGAAGCTGGTTCATAACGGTAACCCGaa                    >  5:5864/1‑149 (MQ=255)                                                                                                                       tgctgAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGGTGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTGAGATggg                                                                 >  1:162953/1‑102 (MQ=255)                                                                                                                       tgctgAAGGTGTATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGGTGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTGAGATggg                                                                 <  2:162953/102‑1 (MQ=255)                                                                                                                                tgtATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTGAGATGGGGAGTCAGGGTGTGGCAATGAAGCTGGTTCATAACGGTAACCCGAATCGCACGCTgg         <  1:218492/149‑1 (MQ=255)                                                                                                                                 gtATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTGAGATGGGTAGTCAGGGTGTGTCAATg                                              >  1:176045/1‑111 (MQ=255)                                                                                                                                 gtATGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTGAGATGGGTAGTCAGGGTGTGTCAATg                                              <  2:176045/111‑1 (MQ=255)                                                                                                                                    tGGTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTGAGATGGGTAGTCAGGGTGTGTCAATGAAGCTGGTTCATAACGGTAACCCGAATCGCACGCTGGCga      <  2:305889/148‑1 (MQ=255)                                                                                                                                      gTCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTGAGATGGGTAGTCAGGGTGTGTCAATGAAGCTGGTTCATAACGGTAACCCGAATCGCACGCTGGCgatga   >  2:99574/1‑149 (MQ=255)                                                                                                                                       tCTGTC‑CTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTGAGATGGGTAGTCAGGGTGTGTCAATGAAGCTGGTTCATAACGGTAACCCGAATCGCACGCTGGCgatgat  <  1:131655/149‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                CGCCAGCAGCAGGGGCTTTCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTCACTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTGAGATGGGTAGTCAGGGTGTGTCAATGAAGCTGGTTCATAACGGTAACCCGAATCGCACGCTGGCGATGAT  >  NC_000913/1361956‑1362239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑