| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,474,813 | 1 | . | T | 26.7% | 20.3 / 15.4 | 15 | coding (593/861 nt) | ydbC | putative NAD(P)‑binding oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base . (7/4); new base T (2/2); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAACAACAAGGCCTGGTAAAACATATTGGCCTGAGCAACGTCACACCGACGCAGGTTGCAGAGGCGCGCAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGC‑TTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGT > NC_000913/1474671‑1474941 | caacaacaaGGCCTGGTAAAACATATTGGCCTGAGCAACGTCTCACCGACGCAGGTTGCAGAGGCGCGCAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGC‑TTTGGc < 8:12379/149‑1 (MQ=255) ttGGCCTGAGCAACGTCACACCGACGCAGGTTGCAGAGGCGCGCAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGC‑TTTGGCCCACGATGGCATTGCCTACGTGCCg > 7:105850/1‑149 (MQ=255) ccTGAGCAACGTCACACCGACGCAGGTTGCAGAGGCGCGCAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATCC‑TTTGGCCCACGATGGCATTGCCTACGTGCCGttct > 1:141898/1‑149 (MQ=255) tCACACCGACGCAGGTTGCAGAGGCGCGCAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGC‑TTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCggg < 8:105850/149‑1 (MQ=255) acCGACGCAGGTTGCAGAGGCGCGCAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCGGATGATGCAATGATTGATGC‑TTTGCCCCACGATGGCATTGCCTACTTGCCGTTCTTCCCGCTCGGGGGCt > 3:86923/1‑149 (MQ=255) aCGCAGGTTGCAGAGGCGCGCAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGCTTTa > 6:4931/1‑149 (MQ=255) aCGCAGGTTGCAGAGGCGCGCAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGCTTTa > 6:7036/1‑149 (MQ=255) aCGCAGGTTGCAGAGGCGCGCAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGC‑TTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGCTTTa > 6:86058/1‑148 (MQ=255) aGGTTGCAGAGGCGCGCAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGC‑TTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCACGGGGGCTTTACACCg > 4:112439/1‑149 (MQ=255) cGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGC‑TTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGCTTTa < 6:135425/119‑1 (MQ=255) aaTTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGtt < 5:4931/149‑1 (MQ=255) aaTTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGtt < 5:7036/149‑1 (MQ=255) tgtgtgCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGC‑TTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAg > 6:29342/1‑149 (MQ=255) tCGCGCACCGTGCTGATGATGCAATGATTGATGC‑TTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAg > 2:54414/1‑149 (MQ=255) atgatgCAATGATTGATGC‑TTTGGCCCACGATGGCATTGCCTACGTGCCGTGCTTCCCGCTCGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGt < 2:141898/147‑1 (MQ=255) | CAACAACAAGGCCTGGTAAAACATATTGGCCTGAGCAACGTCACACCGACGCAGGTTGCAGAGGCGCGCAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGC‑TTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGT > NC_000913/1474671‑1474941 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |