Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 168,660 C→T Q393* (CAG→TAG)  fhuA → ferrichrome outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913168,6600CT99.1% 384.4 / ‑4.9 112Q393* (CAG→TAG) fhuAferrichrome outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (54/57);  total (54/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 112 total aligned reads displayed.

CGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCCAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTGCTCAATCTGTACAATCCGGTGAA  >  NC_000913/168515‑168802
                                                                                                                                                 |                                                                                                                                              
cGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGt                                                                                                                                             <  2:140066/149‑1 (MQ=255)
 gTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGtt                                                                                                                                            <  8:53317/149‑1 (MQ=255)
  tttATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTg                                                                                                                                           >  1:287098/1‑149 (MQ=255)
  attATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTg                                                                                                                                           >  8:39315/2‑149 (MQ=255)
     aTGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCa                                                                                                                                         <  3:124199/148‑1 (MQ=255)
      tGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCTTTATCTGGCACGTAAATACGTCGTTGATGATGAGTAGCTGGAAAACTTCTCCGTTGATACCTAGTTGCag                                                                                                                                        <  7:39315/148‑1 (MQ=255)
        gTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCagag                                                                                                                                      >  5:39648/1‑148 (MQ=255)
        gTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCagag                                                                                                                                      >  2:70896/1‑148 (MQ=255)
          tACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGc                                                                                                                                     >  5:110905/1‑147 (MQ=255)
              gCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGttt                                                                                                                               <  6:39648/149‑1 (MQ=255)
              gCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGttt                                                                                                                               <  8:235048/149‑1 (MQ=255)
                gTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGc                                                                                                                             <  2:303627/149‑1 (MQ=255)
                    gCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACt                                                                                                                         >  1:33336/1‑149 (MQ=255)
                     ctccgctccgGCGACTGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGAAAATACGTCGTTGATGAGGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCa                                                                                                                           <  5:354430/146‑1 (MQ=255)
                     cTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGGGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTg                                                                                                                        <  3:121492/149‑1 (MQ=255)
                     cTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCa                                                                                                                           <  2:287098/146‑1 (MQ=255)
                     cTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCAc                                                                                                                          <  5:18806/147‑1 (MQ=255)
                     cTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTg                                                                                                                        <  8:182573/149‑1 (MQ=255)
                      tCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAACCTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTgg                                                                                                                       >  3:69792/1‑149 (MQ=255)
                      tCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCa                                                                                                                                    >  3:222042/1‑136 (MQ=255)
                      tCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTgg                                                                                                                       >  6:158048/1‑149 (MQ=255)
                        cGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTgg                                                                                                                       <  8:333910/147‑1 (MQ=255)
                        cGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATATAAGGCAACTAAAAACGTACTTTATGAAGAGAAGCTTCAAAAAATAGCCCTTGATACCTTGTTGGAGAGCAAGTTTTCCACTGGCGa                                                                                                                    >  1:53879/1‑149 (MQ=255)
                          aTCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGa                                                                                                                    >  5:37885/1‑148 (MQ=255)
                             cGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATc                                                                                                                >  1:142380/1‑149 (MQ=255)
                              ggCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGGTACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCg                                                                                                               <  1:391500/149‑1 (MQ=255)
                               gCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGa                                                                                                              >  6:301518/1‑149 (MQ=255)
                               gCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGa                                                                                                              <  6:37885/149‑1 (MQ=255)
                                 gAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGAc                                                                                                             >  7:177265/1‑148 (MQ=255)
                                      cTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATAAGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTACTTGCAGAGCAAGTTTGCAACTGGCGATATCGACCacaca                                                                                                       >  2:137579/1‑148 (MQ=255)
                                       ttACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACAccc                                                                                                      >  2:203637/1‑149 (MQ=255)
                                        tACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCct                                                                                                     >  3:278349/1‑149 (MQ=255)
                                         aCAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCct                                                                                                     <  2:33336/148‑1 (MQ=255)
                                               aaCAGTGTGCGGCATTAGCGCCAGCGGATAACGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGAcc                                                                                              <  6:8181/149‑1 (MQ=255)
                                               aaCAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCag                                                                                                                                        >  8:73935/1‑107 (MQ=255)
                                                aCAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCg                                                                                             <  5:158048/149‑1 (MQ=255)
                                                aCAGTGTGCGGCATTAGCGACAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCg                                                                                             <  2:259813/149‑1 (MQ=255)
                                                  aGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGgt                                                                                           <  8:317866/149‑1 (MQ=255)
                                                  aGTGTGCGGCAGTAGCGCCAGCGGATAAAGGCCATTATCTCGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATAGCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGgt                                                                                           <  4:76105/149‑1 (MQ=255)
                                                      tgCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCCGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCg                                                                                       >  8:39184/1‑149 (MQ=255)
                                                            aTTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTta                                                                                 <  1:70896/149‑1 (MQ=255)
                                                                gcgcCATCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTtatgc                                                                              <  3:337040/148‑1 (MQ=255)
                                                                gcgcCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCctg                                                                                                    >  8:405227/1‑126 (MQ=255)
                                                                 cgcCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTtatgcgt                                                                            <  2:142380/149‑1 (MQ=255)
                                                                 cgcCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTtatgcgt                                                                            <  3:32401/149‑1 (MQ=255)
                                                                 cgcCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTtatgcg                                                                             >  3:133200/1‑148 (MQ=255)
                                                                 cgcCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTtatgcg                                                                             >  7:221268/1‑148 (MQ=255)
                                                                   ccATCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTtatgcgtat                                                                          <  2:27758/149‑1 (MQ=255)
                                                                    cAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTtatgcgtatg                                                                         >  4:227495/1‑149 (MQ=255)
                                                                       cGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCAGACCCTGCTGACCGGTGTCGACTTTatgcgtatgcgt                                                                      <  1:137579/149‑1 (MQ=255)
                                                                        ggATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTtatg                                                                               <  4:241540/139‑1 (MQ=255)
                                                                        ggATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTtatg                                                                               >  3:241540/1‑139 (MQ=255)
                                                                          aTAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAAt                                                                   <  4:278349/149‑1 (MQ=255)
                                                                          aTAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAAt                                                                   <  6:110905/149‑1 (MQ=255)
                                                                           tAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAAt                                                                   >  7:294082/1‑148 (MQ=255)
                                                                              aGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTaa                                                                    <  6:375186/144‑1 (MQ=255)
                                                                              aGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACa                                                               >  2:343539/1‑149 (MQ=255)
                                                                              aGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACAACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTaa                                                                    >  5:375186/1‑144 (MQ=255)
                                                                                gCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGATGACCGGTGTCGACTTTATGCGTATGCGTAATGACAt                                                              >  2:112133/1‑148 (MQ=255)
                                                                                 ccATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCCCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATc                                                             >  3:11897/1‑148 (MQ=255)
                                                                                  cATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAAt                                                                   >  2:290594/1‑141 (MQ=255)
                                                                                             aCGTAAATACGTCGTTGATGATTAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCtggtttg                                                <  6:59068/149‑1 (MQ=255)
                                                                                             aCGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGAc                                                                                                             >  1:22764/1‑88 (MQ=255)
                                                                                             aCGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGAc                                                                                                             <  2:22764/88‑1 (MQ=255)
                                                                                             aCGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCtggtttg                                                >  2:196593/1‑149 (MQ=255)
                                                                                             aCGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCtggtttg                                                >  7:7490/1‑149 (MQ=255)
                                                                                              cGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCtggtttgg                                               <  8:221268/149‑1 (MQ=255)
                                                                                              cGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCtggtttgg                                               <  2:394930/149‑1 (MQ=255)
                                                                                                     aCGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCTTATGCGTAATGACATCAACGCCTGGTTTGGTTacgac                                        >  6:86677/1‑149 (MQ=255)
                                                                                                           ttGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGAGTAATGACATCAACGCCTGGTTTGGTTTCGCCGActct                                  >  6:115122/1‑149 (MQ=255)
                                                                                                              atgatgaGAAGCTTCAAAACTTCTCCGTTGATAACTAGTTGCAGAGCAAGTTTTACACTGGCGATATCGACCACACCCTGCTGACCGTTGTCGACTTTCTGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCtgt                                <  6:194982/148‑1 (MQ=255)
                                                                                                              atgatgaGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCtggtttg                                                >  8:208447/1‑132 (MQ=255)
                                                                                                              atgatgaGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCtggtttg                                                <  7:208447/132‑1 (MQ=255)
                                                                                                                gatgaGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGc                              >  4:135568/1‑148 (MQ=255)
                                                                                                                  tgaGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGAc                                                                >  1:148648/1‑112 (MQ=255)
                                                                                                                  tgaGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGAc                                                                <  2:148648/112‑1 (MQ=255)
                                                                                                                  tgaGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCAc                           >  8:206328/1‑149 (MQ=255)
                                                                                                                   gagaAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCAc                           <  8:100020/148‑1 (MQ=255)
                                                                                                                   gagaAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCAc                           >  7:100020/1‑148 (MQ=255)
                                                                                                                    agaAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTg                         >  7:120199/1‑149 (MQ=255)
                                                                                                                     gaAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTGc                        >  1:233265/1‑149 (MQ=255)
                                                                                                                     gaAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTGc                        >  1:96171/1‑149 (MQ=255)
                                                                                                                       aGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCtgt                                >  6:313426/1‑139 (MQ=255)
                                                                                                                           gCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTtatgcgtat                                                                          >  5:214671/1‑93 (MQ=255)
                                                                                                                            cAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTGCTCAATc                  <  3:227495/148‑1 (MQ=255)
                                                                                                                            cAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTGCTCAATCt                 >  1:188054/1‑149 (MQ=255)
                                                                                                                              aaaCTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTGCTCAATCTg                >  2:346320/1‑148 (MQ=255)
                                                                                                                               aaCTTCTCCTTTGATACCTCGGTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTGCTCAATCTGTa              <  4:189128/149‑1 (MQ=255)
                                                                                                                               aaCTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTGCTCAATCTGTa              <  8:7490/149‑1 (MQ=255)
                                                                                                                               aaCTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTGCTCAATCTGTa              <  1:203637/149‑1 (MQ=255)
                                                                                                                                       cGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTGCTCAATCTGTACAATc         <  1:346320/146‑1 (MQ=255)
                                                                                                                                       cGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTGCTCAATCTGTACAATc         <  7:39184/146‑1 (MQ=255)
                                                                                                                                        gTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCtggtttg                                                >  1:132315/1‑106 (MQ=255)
                                                                                                                                        gTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCtggtttg                                                <  2:132315/106‑1 (MQ=255)
                                                                                                                                        gTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTGCTCAATCTGTACAATCCGGt     >  4:175308/1‑149 (MQ=255)
                                                                                                                                        gTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGGCGACTTTATGCGTATGCGTAATGAAATCAACGCCTGGTTTGGGTACGACGACTCTGTGCCACTGCTCAATCTGTACAATCCGGt     <  4:69792/149‑1 (MQ=255)
                                                                                                                                        gTTGATACCCAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGGGGCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTGCTCAATCTGTACAATCCGGt     <  3:314112/149‑1 (MQ=255)
                                                                                                                                           gATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTGCTCAATCTGTACAATCCGGTGaa  >  7:281444/1‑149 (MQ=255)
                                                                                                                                            aTACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTGCTCAATCTGTACAATCCGGTGaa  >  1:315339/1‑148 (MQ=255)
                                                                                                                                              aCCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTGCTCAATCTGTACAATCCgg      >  7:78777/1‑142 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
CGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCCAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTGCTCAATCTGTACAATCCGGTGAA  >  NC_000913/168515‑168802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: