Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,667,529 T→A K346* (AAA→TAA)  mlc ← glucosamine anaerobic growth regulon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,667,5290TA98.6% 241.3 / ‑4.1 70K346* (AAA→TAA) mlcglucosamine anaerobic growth regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (38/31);  total (38/32)
Fisher's exact test for biased strand distribution p-value = 4.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

ATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTC  >  NC_000913/1667384‑1667673
                                                                                                                                                 |                                                                                                                                                
aTCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGCCTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACt                                                                                                                                               >  1:292985/1‑149 (MQ=255)
aTCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACt                                                                                                                                               >  3:128157/1‑149 (MQ=255)
 tCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACt                                                                                                                                               >  2:401033/1‑148 (MQ=255)
    cgTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCttaacttaac                                                                                                                                           >  1:285943/1‑149 (MQ=255)
     gTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACg                                                                                                                                          <  2:97849/149‑1 (MQ=255)
      tCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTCGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACgg                                                                                                                                         >  6:280255/1‑149 (MQ=255)
         tttACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGt                                                                                                                                        >  3:18217/1‑147 (MQ=255)
          ttACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGt                                                                                                                                        <  1:161743/146‑1 (MQ=255)
           tACCAGTGCAGCGCCTGCAATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGc                                                                                                                                    >  4:189927/1‑149 (MQ=255)
              cAGTGCAGCGCCTGCGATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCaa                                                                                                                                 <  3:189927/149‑1 (MQ=255)
                gTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAAt                                                                                                                                >  5:238827/1‑148 (MQ=255)
                   cAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCGTAACTTAACGGTGAGCCAATCAg                                                                                                                             >  1:244696/1‑148 (MQ=255)
                         cTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTAttttt                                                                                                                      >  1:20369/1‑149 (MQ=255)
                            ccATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTtg                                                                                                                    <  5:203580/148‑1 (MQ=255)
                            ccATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTtgt                                                                                                                   <  2:292985/149‑1 (MQ=255)
                              aTCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTgg                                                                                                                 >  4:348006/1‑149 (MQ=255)
                                       tGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTtaaata                                                                                                         >  5:286661/1‑148 (MQ=255)
                                       tGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGCTtaaata                                                                                                         >  5:287374/1‑148 (MQ=255)
                                         gTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTtaaataaa                                                                                                       >  5:43552/1‑148 (MQ=255)
                                               aaaCTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCa                                                                                                <  5:211920/149‑1 (MQ=255)
                                                aaCTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCat                                                                                               <  6:162951/149‑1 (MQ=255)
                                                   tGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGCGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCatgat                                                                                            >  4:259108/1‑149 (MQ=255)
                                                     aGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCatgatg                                                                                           <  5:265393/148‑1 (MQ=255)
                                                      gTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATgg                                                                                          >  1:55195/1‑148 (MQ=255)
                                                      gTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGc                                                                                         <  1:401033/149‑1 (MQ=255)
                                                         ctctCAACGCTGATGTGCTGCCTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGc                                                                                         >  2:41863/1‑146 (MQ=255)
                                                         ctctCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCaa                                                                                       <  1:385073/148‑1 (MQ=255)
                                                            tcAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAAt                                                                                   >  4:130548/1‑149 (MQ=255)
                                                              aaCGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGaa                                                                                    <  3:270889/146‑1 (MQ=255)
                                                              aaCGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAAt                                                                                   <  3:359477/147‑1 (MQ=255)
                                                              aaCGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAAt                                                                                   <  4:128157/147‑1 (MQ=255)
                                                              aaCGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATgc                                                                                 >  2:15794/1‑149 (MQ=255)
                                                               aCGCTGATGTGCTGACGATACGCAGGAAGGGCCTGCTGACGGATACTGTCTGAGATGACCGGGAAGAGGATATCTGCCGcttaacttaa                                                                                                                                            >  8:3295/1‑89 (MQ=255)
                                                               aCGCTGATGTGCTGACGATACGCAGGAAGGGCCTGCTGACGGATACTGTCTGAGATGACCGGGAAGAGGATATCTGCCGcttaacttaa                                                                                                                                            <  7:3295/89‑1 (MQ=255)
                                                                 gCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCa                                                                                        >  2:195305/1‑139 (MQ=255)
                                                                 gCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCa                                                                                        <  1:195305/139‑1 (MQ=255)
                                                                 gCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGcc                                                                              <  4:307777/149‑1 (MQ=255)
                                                                     aTGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCg                                                                            <  2:285943/147‑1 (MQ=255)
                                                                       gtgCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACAt                                                                        >  1:386863/1‑149 (MQ=255)
                                                                          cTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATgc                                                                      <  5:66566/148‑1 (MQ=255)
                                                                                         aaGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTaa                                                      <  2:386863/149‑1 (MQ=255)
                                                                                             gCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATg                                                                                  <  5:231866/117‑1 (MQ=255)
                                                                                             gCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATg                                                                                  >  6:231866/1‑117 (MQ=255)
                                                                                             gCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAatgat                                                  <  1:41863/149‑1 (MQ=255)
                                                                                               ctgctgACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGt                                                >  3:313170/1‑149 (MQ=255)
                                                                                                 gctgACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAat                                                     >  6:395242/1‑142 (MQ=255)
                                                                                                 gctgACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAat                                                     <  5:395242/142‑1 (MQ=255)
                                                                                                  ctgACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCtt                                             <  2:55195/149‑1 (MQ=255)
                                                                                                     aCGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCtttt                                           <  3:130548/148‑1 (MQ=255)
                                                                                                     aCGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCttt                                            <  5:280255/147‑1 (MQ=255)
                                                                                                      cGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGc                                         <  1:15794/149‑1 (MQ=255)
                                                                                                       ggATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCatga                                                                                             >  4:271588/1‑96 (MQ=255)
                                                                                                       ggATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCatga                                                                                             <  3:271588/96‑1 (MQ=255)
                                                                                                       ggATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGcc                                        <  4:18217/149‑1 (MQ=255)
                                                                                                       ggATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGc                                         >  4:216544/1‑148 (MQ=255)
                                                                                                               tCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCtttt                                           >  6:42224/1‑138 (MQ=255)
                                                                                                               tCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCtttt                                           <  5:42224/138‑1 (MQ=255)
                                                                                                                  gagaTGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATcgcc                             >  7:72012/1‑149 (MQ=255)
                                                                                                                    gaTGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATcgccgc                           >  3:53108/1‑149 (MQ=255)
                                                                                                                      tGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCcgcg                          >  6:257628/1‑148 (MQ=255)
                                                                                                                        aCCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCaa                       >  1:217042/1‑149 (MQ=255)
                                                                                                                          cGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATg                     >  7:7432/1‑149 (MQ=255)
                                                                                                                                gagGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCt               >  3:33671/1‑149 (MQ=255)
                                                                                                                                 agGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCt               >  1:282609/1‑148 (MQ=255)
                                                                                                                                   gATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGa             >  1:262256/1‑148 (MQ=255)
                                                                                                                                      atCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGacac          >  2:395759/1‑148 (MQ=255)
                                                                                                                                          gCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATgccgc                 >  2:109568/1‑137 (MQ=255)
                                                                                                                                          gCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATgccgc                 <  1:109568/137‑1 (MQ=255)
                                                                                                                                           ccGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAAt       <  4:59991/146‑1 (MQ=255)
                                                                                                                                           ccGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGa     <  3:259108/148‑1 (MQ=255)
                                                                                                                                            cGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCg                                                          >  1:400286/1‑94 (MQ=255)
                                                                                                                                            cGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCg                                                          <  2:400286/94‑1 (MQ=255)
                                                                                                                                             gCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTc  >  8:210330/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTC  >  NC_000913/1667384‑1667673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: